The identification of germplasm is an important step for effective utilization of the available germplasm. In previous studies, isoenzyme, RAPD and SSR techniques had been used to conduct the genetic identification of...The identification of germplasm is an important step for effective utilization of the available germplasm. In previous studies, isoenzyme, RAPD and SSR techniques had been used to conduct the genetic identification of watermelon ( Citrullus lanatus (Thunb.) Mansf.), but their effectiveness was limited due to the extremely narrow genetic background among watermelon genotypes. In this research, amplified fragment length polymorphism (AFLP), which was reported as a reliable technique with high efficiency in detecting polymorphism, was used to conduct genetic analysis and variety identification of thirty genotypes of watermelon core collection that represent a wide range of breeding and commercially available germplasm. As a result, a DNA fingerprint based on 15 bands amplified with four primer combinations was developed. In this fingerprint, each genotype has its unique fingerprint pattern and can be distinguished from each other. Furthermore, in or der to facilitate the utilization of AFLP marker in practice, one specific AFLP band of genotype 'PI296341' coming from fragment amplified by primer combination E-AT/M-CAT was successfully converted into a sequence characterized amplified region (SCAR) marker.展开更多
[Objective] The aim of this study was to characterize the national regis- tered varieties selected from cotton regional trials in Yangtze River Valley (YaRV) in recent years. [Method] Cotton cultivar classification ...[Objective] The aim of this study was to characterize the national regis- tered varieties selected from cotton regional trials in Yangtze River Valley (YaRV) in recent years. [Method] Cotton cultivar classification and comprehensive evaluation index were set up based on national cotton registration standard. GGE biplot method was adopted to analyze the correlation of major breeding target characters of 53 national registered cotton varieties in cotton regional trials in YaRV during 1981-2012. According to the shift of check cultivars in cotton regional trials in the past, the cotton regional trial practice since 1981 was divided into five periods. The dynamic of cultivar type's proportion and the evaluation index scores was analyzed across the five periods. [Result] There existed intricate interrelationship among cotton breeding target traits, which constrained it necessary to construct indices for com- prehensive evaluation of cotton varieties. The dynamic of cultivar types in the five periods indicated that type II varieties emerged since Simian 3 period and then its proportion decreased gradually; type Ⅲ varieties maintained a certain proportion in each period and kept on the rise overall; type Ⅳvarieties occupied the majority pro- portion of registered cultivars before 1993, but a minor proportion since Simian 3 period. On the other side, the change trend of the evaluation index demonstrated that the varieties registered before 2003 did not pass the qualified line at present. The peak scores appeared in the varieties registered during 2004-2008. The scores of the varieties registered after 2009 were only slightly over the qualified line. [Conclusion] More attention should be paid to the improvement and evaluation of micronaire, so as to guide the simultaneous development of high yielding and fiber quality in cotton breeding and registration procedure in YaRV.展开更多
[Objective] Taking the characteristic of flower diameter of Tagetes L.as an example,this study aimed to select example varieties used in the DUS Test Guideline of Tagetes L.[Method] Two continuous years of measurement...[Objective] Taking the characteristic of flower diameter of Tagetes L.as an example,this study aimed to select example varieties used in the DUS Test Guideline of Tagetes L.[Method] Two continuous years of measurements of flower diameter of 25 varieties were collected and then analyzed by using the box plot to illustrate the uniformity and stability of flower diameter of each variety.[Result] According to the information of variability,distribution symmetry of measurements and outliers of flower diameter of varieties provided by box plots,variety 16,2 and 4 were selected as the example varieties for the three expression states with respective flower diameter of 3.0-4.4,6.0-7.4 and 9.0-10.4 cm.[Conclusion] The box plot is an efficient method for the general analysis of varieties,which provides information covering the actual and possible expression range,median and outliers of measurements of flower diameter of each variety.It also provides references for selecting example varieties for other quantitative characteristics and evaluating the quality of varieties.展开更多
Extreme weather events were analyzed based on the meteorological data from the year of 1967 to 2007 for Yamaguchi, Japan. The responses from landscape trees were also investigated mainly by the analysis of image pixel...Extreme weather events were analyzed based on the meteorological data from the year of 1967 to 2007 for Yamaguchi, Japan. The responses from landscape trees were also investigated mainly by the analysis of image pixel and spectral reflectance. Results show that after the dry, hot and windy summer in 2007, many landscape trees in Yamaguchi City tended to respond the extreme weather events by reducing their leaf surface area and receiving less radiation energy. Premature leaf discoloration or defoliation appeared on some landscape tree species and leaf necrosis occurred on tip and margin of many Kousa dogwood (Cornus kousa) trees at unfavorable sites. Described by image pixel analysis method, the leaf necrotic area percentage (LNAP) of sampled dogwood trees averaged 41.6% and the sampled Sasanqua camellia (Camelia sasanqua) tree also showed fewer flowers in flower season of 2007 than that in 2006. By differential analysis of partial discolored crown, it presented a logistic differential equation of crown color for sweet gum (Liquidambar styraciflua) trees. It suggested that the persistent higher temperature and lower precipitation could be injurious to the sensitive landscape trees at poor sites, even in relative humid area like Yamaguchi.展开更多
Variety identification is important for maize breeding, processing and trade. The computer vision technique has been widely applied to maize variety identification. In this paper, computer vision technique has been su...Variety identification is important for maize breeding, processing and trade. The computer vision technique has been widely applied to maize variety identification. In this paper, computer vision technique has been summarized from the following technical aspects including image acquisition, image processing, characteristic parameter extraction, pattern recognition and programming softwares. In addition, the existing problems during the application of this technique to maize variety identification have also been analyzed and its development tendency is forecasted.展开更多
This study was to evaluate the high yielding and stability of candidate cultivars, depict the adaptive planting region, analyze trial location discrim-ination ability and representativeness, as wel as identify the ide...This study was to evaluate the high yielding and stability of candidate cultivars, depict the adaptive planting region, analyze trial location discrim-ination ability and representativeness, as wel as identify the ideal cultivar and trial location, with the aim to provide theory background for cultivar selection and rea-sonable scheme of test location in Jiangsu Province. [Method] The GGE biplot method was used to analyze the lint cotton yield of 12 experimental genotypes in the 6 test locations (three replicates in each) of the cotton regional trial in Jiangsu Province in 2013. [Result] The effects of genotype (G), environment (E), and geno-type by environment interaction (G×E) on lint cotton yield were al highly significant (P〈0.01), which made it necessary to further explore the specific pattern of geno-type by environment interaction. Jinmian118 (G4) and SF3303 (G5) were the best ideal genotypes screened by the "ideal cultivar" and "ideal location" view of GGE biplot, and the ordination of test sites based on the ideal index were in the order of Dafeng (DF), Yanliang (YL), Liuhe (LH), Dongtai (DT), Yancheng (YC), and Nantong (NT), among which NT was relatively weak in representing of the whole target cot-ton planting region in Jiangsu Province. The "similarity among locations" view of GGE biplot clustered al trial locations into one group, showing that the test sites in the cotton planting region in Jiangsu Province were in the same mega-environment. The "which-won-where" view of GGE biplot indicated that cotton cultivar Jinmian118 (G4) was the most appropriate cultivar in the homogeneous cotton planting region in Jiangsu Province. [Conclusion] Among the candidate cultivars, Jinmian118 and SF3303 were identified as the most ideal cultivars in this set of conventional cotton regional trial in Jiangsu Province; the test site of Dafeng ranked the first out of al locations in terms of discrimination and representativeness, and al test locations were clustered into the same mega-environmet, which indicated the high efficiency of cultivar selection in the cotton regional trial in Jiangsu Province.展开更多
On the basis of an electronic map_based hypermedia data model (EMBHDM),this paper makes a study on the technologies of nonlinear storage,organization,management and browsing of information as well as organization of d...On the basis of an electronic map_based hypermedia data model (EMBHDM),this paper makes a study on the technologies of nonlinear storage,organization,management and browsing of information as well as organization of data on the basis of the relationship between multimedia information of electronic maps. This paper first analyzes some features of electronic maps and hypermedia technology,and then illustrates the creation of EMBHDM with some examples.The tests show that this model can efficiently organize and express multimedia data.展开更多
[Objective] The paper analyzed production adaptability and stability of 4 hybrids of castor,so as to explore the application method of AMMI model in variety evaluation.[Method] AMMI model and biplot were used to carry...[Objective] The paper analyzed production adaptability and stability of 4 hybrids of castor,so as to explore the application method of AMMI model in variety evaluation.[Method] AMMI model and biplot were used to carry out stability analysis on 4 castor combinations in multi-point test during 2008 and 2009,and further evaluate the stability and adaptability of various tested combinations.[Result] AMMI model analysis showed that the singular values IPCA1 and IPCA2 in principal component axis of interaction totally explained 92.24% of the interaction variance.AMMI model not only maximizes the reflection on variation of interaction,but also can accurately analyze the adaptability and yield stability of varieties.[Conclusion] AMMI model integrates variance analysis and principal component analysis together,which improves accuracy compared with the traditional method.Even the adaptability of female line without fertility could be indirectly evaluated through the identification on the genotype and environment interaction effects in F1 generation.展开更多
[Objective] The aim was to study the molecular identification and cultivar fingerprints of Prunus persica (L.) Batsch germplasms.[Method] Sixty peach genotypes,representing China common local cultivars and European sa...[Objective] The aim was to study the molecular identification and cultivar fingerprints of Prunus persica (L.) Batsch germplasms.[Method] Sixty peach genotypes,representing China common local cultivars and European samples were screened by microsatellites (simple sequence repeats,SSRs) and Inter-Simple Sequence Repeat (ISSR) markers.[Result] 26 reproducible bands were amplified by Nine SSR primers,and 24 of which were polymorphic; 236 bands were amplified by 30 ISSR primers,and 113 of which were polymorphic.31 genotypes were discriminated with 1-3 distinct polymorphic bands generated from the primers ISSR and SSR.Seven cultivar-specific ISSR fragments and two SSR unique alleles obtained from this study were available to be converted into Sequence Characterized Amplified Region (SCAR) markers.The genetic similarity coefficient (GS) estimated from these molecular data averaged were 0.939 (ranged from 0.856 to 0.983) for ISSR and 0.646 (ranged from 0.240 to 1.000) for SSR,respectively.The combined grouping association indicated that most local Chinese peach cultivars and exotic accessions were clustered together.This could be related to the mode of introduction and maintenance of the peach cultivars involving limited foundation germplasm,exchange of cultivars between plantations,and periodic development of new recombinant cultivars following sexual reproduction.[Conclusion] The results obtained in this work would help to improve the conservation,molecular identification and management of peach germplasm in breeding.展开更多
Segmenting blurred and conglutinated bubbles in a flotation image is done using a new segmentation method based on Seed Region and Boundary Growing(SRBG).Bright pixels located on bubble tops were extracted as the se...Segmenting blurred and conglutinated bubbles in a flotation image is done using a new segmentation method based on Seed Region and Boundary Growing(SRBG).Bright pixels located on bubble tops were extracted as the seed regions.Seed boundaries are divided into four curves:left-top,right-top,right-bottom, and left-bottom.Bubbles are segmented from the seed boundary by moving these curves to the bubble boundaries along the corresponding directions.The SRBG method can remove noisy areas and it avoids over- and under-segmentation problems.Each bubble is segmented separately rather than segmenting the entire flotation image.The segmentation results from the SRBG method are more accurate than those from the Watershed algorithm.展开更多
trast (HC) method is proposed to define saliency value of each pixel, then auto Grabcut segmenta- tion method is used to segment the salient region so as to obtain a region of interest (ROI). After that, normalize...trast (HC) method is proposed to define saliency value of each pixel, then auto Grabcut segmenta- tion method is used to segment the salient region so as to obtain a region of interest (ROI). After that, normalized histograms and cumulative histograms for ROI and region of background (ROB) are calculated. The mapping functions of the corresponding regions are derived from reference image to distorted image through the nearest cumulative histogram matching method, so that color correction can be finally achieved. Experimental results show that benefitting from the separate treatment to ROI and ROB, the proposed color correction method could avoid error propagation between the two different regions, which achieves good color correction result in comparison with other correction methods.展开更多
文摘The identification of germplasm is an important step for effective utilization of the available germplasm. In previous studies, isoenzyme, RAPD and SSR techniques had been used to conduct the genetic identification of watermelon ( Citrullus lanatus (Thunb.) Mansf.), but their effectiveness was limited due to the extremely narrow genetic background among watermelon genotypes. In this research, amplified fragment length polymorphism (AFLP), which was reported as a reliable technique with high efficiency in detecting polymorphism, was used to conduct genetic analysis and variety identification of thirty genotypes of watermelon core collection that represent a wide range of breeding and commercially available germplasm. As a result, a DNA fingerprint based on 15 bands amplified with four primer combinations was developed. In this fingerprint, each genotype has its unique fingerprint pattern and can be distinguished from each other. Furthermore, in or der to facilitate the utilization of AFLP marker in practice, one specific AFLP band of genotype 'PI296341' coming from fragment amplified by primer combination E-AT/M-CAT was successfully converted into a sequence characterized amplified region (SCAR) marker.
基金Supported by Key Special Project for Breeding and Cultivation of GMO Varieties(2012ZX08013015)Jiangsu Agriculture Science and Technology Innovation Fund(JASTIF,CX-12-5035)~~
文摘[Objective] The aim of this study was to characterize the national regis- tered varieties selected from cotton regional trials in Yangtze River Valley (YaRV) in recent years. [Method] Cotton cultivar classification and comprehensive evaluation index were set up based on national cotton registration standard. GGE biplot method was adopted to analyze the correlation of major breeding target characters of 53 national registered cotton varieties in cotton regional trials in YaRV during 1981-2012. According to the shift of check cultivars in cotton regional trials in the past, the cotton regional trial practice since 1981 was divided into five periods. The dynamic of cultivar type's proportion and the evaluation index scores was analyzed across the five periods. [Result] There existed intricate interrelationship among cotton breeding target traits, which constrained it necessary to construct indices for com- prehensive evaluation of cotton varieties. The dynamic of cultivar types in the five periods indicated that type II varieties emerged since Simian 3 period and then its proportion decreased gradually; type Ⅲ varieties maintained a certain proportion in each period and kept on the rise overall; type Ⅳvarieties occupied the majority pro- portion of registered cultivars before 1993, but a minor proportion since Simian 3 period. On the other side, the change trend of the evaluation index demonstrated that the varieties registered before 2003 did not pass the qualified line at present. The peak scores appeared in the varieties registered during 2004-2008. The scores of the varieties registered after 2009 were only slightly over the qualified line. [Conclusion] More attention should be paid to the improvement and evaluation of micronaire, so as to guide the simultaneous development of high yielding and fiber quality in cotton breeding and registration procedure in YaRV.
基金Supported by the Special Fund for Agro-scientific Research in the Public Interest(200903008-14)the National "948" Project(2009-Z11)~~
文摘[Objective] Taking the characteristic of flower diameter of Tagetes L.as an example,this study aimed to select example varieties used in the DUS Test Guideline of Tagetes L.[Method] Two continuous years of measurements of flower diameter of 25 varieties were collected and then analyzed by using the box plot to illustrate the uniformity and stability of flower diameter of each variety.[Result] According to the information of variability,distribution symmetry of measurements and outliers of flower diameter of varieties provided by box plots,variety 16,2 and 4 were selected as the example varieties for the three expression states with respective flower diameter of 3.0-4.4,6.0-7.4 and 9.0-10.4 cm.[Conclusion] The box plot is an efficient method for the general analysis of varieties,which provides information covering the actual and possible expression range,median and outliers of measurements of flower diameter of each variety.It also provides references for selecting example varieties for other quantitative characteristics and evaluating the quality of varieties.
文摘Extreme weather events were analyzed based on the meteorological data from the year of 1967 to 2007 for Yamaguchi, Japan. The responses from landscape trees were also investigated mainly by the analysis of image pixel and spectral reflectance. Results show that after the dry, hot and windy summer in 2007, many landscape trees in Yamaguchi City tended to respond the extreme weather events by reducing their leaf surface area and receiving less radiation energy. Premature leaf discoloration or defoliation appeared on some landscape tree species and leaf necrosis occurred on tip and margin of many Kousa dogwood (Cornus kousa) trees at unfavorable sites. Described by image pixel analysis method, the leaf necrotic area percentage (LNAP) of sampled dogwood trees averaged 41.6% and the sampled Sasanqua camellia (Camelia sasanqua) tree also showed fewer flowers in flower season of 2007 than that in 2006. By differential analysis of partial discolored crown, it presented a logistic differential equation of crown color for sweet gum (Liquidambar styraciflua) trees. It suggested that the persistent higher temperature and lower precipitation could be injurious to the sensitive landscape trees at poor sites, even in relative humid area like Yamaguchi.
基金Special Fund for Science & Technology Research of Education Commission,Chongqing(KJ101302)~~
文摘Variety identification is important for maize breeding, processing and trade. The computer vision technique has been widely applied to maize variety identification. In this paper, computer vision technique has been summarized from the following technical aspects including image acquisition, image processing, characteristic parameter extraction, pattern recognition and programming softwares. In addition, the existing problems during the application of this technique to maize variety identification have also been analyzed and its development tendency is forecasted.
基金Supported by the Fund for Independent Innovation of Agricultural Sciences in Jiangsu Province(CX-12-5035)
文摘This study was to evaluate the high yielding and stability of candidate cultivars, depict the adaptive planting region, analyze trial location discrim-ination ability and representativeness, as wel as identify the ideal cultivar and trial location, with the aim to provide theory background for cultivar selection and rea-sonable scheme of test location in Jiangsu Province. [Method] The GGE biplot method was used to analyze the lint cotton yield of 12 experimental genotypes in the 6 test locations (three replicates in each) of the cotton regional trial in Jiangsu Province in 2013. [Result] The effects of genotype (G), environment (E), and geno-type by environment interaction (G×E) on lint cotton yield were al highly significant (P〈0.01), which made it necessary to further explore the specific pattern of geno-type by environment interaction. Jinmian118 (G4) and SF3303 (G5) were the best ideal genotypes screened by the "ideal cultivar" and "ideal location" view of GGE biplot, and the ordination of test sites based on the ideal index were in the order of Dafeng (DF), Yanliang (YL), Liuhe (LH), Dongtai (DT), Yancheng (YC), and Nantong (NT), among which NT was relatively weak in representing of the whole target cot-ton planting region in Jiangsu Province. The "similarity among locations" view of GGE biplot clustered al trial locations into one group, showing that the test sites in the cotton planting region in Jiangsu Province were in the same mega-environment. The "which-won-where" view of GGE biplot indicated that cotton cultivar Jinmian118 (G4) was the most appropriate cultivar in the homogeneous cotton planting region in Jiangsu Province. [Conclusion] Among the candidate cultivars, Jinmian118 and SF3303 were identified as the most ideal cultivars in this set of conventional cotton regional trial in Jiangsu Province; the test site of Dafeng ranked the first out of al locations in terms of discrimination and representativeness, and al test locations were clustered into the same mega-environmet, which indicated the high efficiency of cultivar selection in the cotton regional trial in Jiangsu Province.
文摘On the basis of an electronic map_based hypermedia data model (EMBHDM),this paper makes a study on the technologies of nonlinear storage,organization,management and browsing of information as well as organization of data on the basis of the relationship between multimedia information of electronic maps. This paper first analyzes some features of electronic maps and hypermedia technology,and then illustrates the creation of EMBHDM with some examples.The tests show that this model can efficiently organize and express multimedia data.
基金Supported by Science and Technology Research Project of Shan-dong Province"Research on Saline-alkali Breeding and Promotion of Castor Oil Plant"(2007GG3NS9014)~~
文摘[Objective] The paper analyzed production adaptability and stability of 4 hybrids of castor,so as to explore the application method of AMMI model in variety evaluation.[Method] AMMI model and biplot were used to carry out stability analysis on 4 castor combinations in multi-point test during 2008 and 2009,and further evaluate the stability and adaptability of various tested combinations.[Result] AMMI model analysis showed that the singular values IPCA1 and IPCA2 in principal component axis of interaction totally explained 92.24% of the interaction variance.AMMI model not only maximizes the reflection on variation of interaction,but also can accurately analyze the adaptability and yield stability of varieties.[Conclusion] AMMI model integrates variance analysis and principal component analysis together,which improves accuracy compared with the traditional method.Even the adaptability of female line without fertility could be indirectly evaluated through the identification on the genotype and environment interaction effects in F1 generation.
基金Supported by National Peach Industrial Technology System (nycytx-31-zs-10 )National Science and Technology Support Program (2008BAD98B03-08)+1 种基金National Peach Commonweal Science (Agriculture) Research Projects (3-37)Chengdu Technology Application and Promotion Program (09YTZD986NC-012)
文摘[Objective] The aim was to study the molecular identification and cultivar fingerprints of Prunus persica (L.) Batsch germplasms.[Method] Sixty peach genotypes,representing China common local cultivars and European samples were screened by microsatellites (simple sequence repeats,SSRs) and Inter-Simple Sequence Repeat (ISSR) markers.[Result] 26 reproducible bands were amplified by Nine SSR primers,and 24 of which were polymorphic; 236 bands were amplified by 30 ISSR primers,and 113 of which were polymorphic.31 genotypes were discriminated with 1-3 distinct polymorphic bands generated from the primers ISSR and SSR.Seven cultivar-specific ISSR fragments and two SSR unique alleles obtained from this study were available to be converted into Sequence Characterized Amplified Region (SCAR) markers.The genetic similarity coefficient (GS) estimated from these molecular data averaged were 0.939 (ranged from 0.856 to 0.983) for ISSR and 0.646 (ranged from 0.240 to 1.000) for SSR,respectively.The combined grouping association indicated that most local Chinese peach cultivars and exotic accessions were clustered together.This could be related to the mode of introduction and maintenance of the peach cultivars involving limited foundation germplasm,exchange of cultivars between plantations,and periodic development of new recombinant cultivars following sexual reproduction.[Conclusion] The results obtained in this work would help to improve the conservation,molecular identification and management of peach germplasm in breeding.
基金supported in part by the National Science & Technology Support Plan of China(No.2009BAB48B02)
文摘Segmenting blurred and conglutinated bubbles in a flotation image is done using a new segmentation method based on Seed Region and Boundary Growing(SRBG).Bright pixels located on bubble tops were extracted as the seed regions.Seed boundaries are divided into four curves:left-top,right-top,right-bottom, and left-bottom.Bubbles are segmented from the seed boundary by moving these curves to the bubble boundaries along the corresponding directions.The SRBG method can remove noisy areas and it avoids over- and under-segmentation problems.Each bubble is segmented separately rather than segmenting the entire flotation image.The segmentation results from the SRBG method are more accurate than those from the Watershed algorithm.
基金Supported by the Natural Science Foundation of China(No.61311140262,61171163,61271021)
文摘trast (HC) method is proposed to define saliency value of each pixel, then auto Grabcut segmenta- tion method is used to segment the salient region so as to obtain a region of interest (ROI). After that, normalized histograms and cumulative histograms for ROI and region of background (ROB) are calculated. The mapping functions of the corresponding regions are derived from reference image to distorted image through the nearest cumulative histogram matching method, so that color correction can be finally achieved. Experimental results show that benefitting from the separate treatment to ROI and ROB, the proposed color correction method could avoid error propagation between the two different regions, which achieves good color correction result in comparison with other correction methods.