鼻下端圆隆肥大的鼻成形术

目的 通过对鼻下端软组织各层血管密度的研究 ,探讨治疗鼻下端圆隆肥大的手术方法。方法 取新鲜尸体头颅 ,经甲醛墨汁液灌注 ,于鼻端、鼻背、面部、颈部取材 ,制成石蜡切片 ,伊红染色 ;图象分析法测量各层微血管密度。根据 18例患者鼻下端圆隆肥大表现 ,分别采用A类方法 :膨体聚四氟乙烯假体置入隆鼻术 +鼻翼切薄术 +缝合法鼻翼沟成形术 ;B类方法 :鼻翼浅筋膜切除术 +肋软骨移植隆鼻术 +鼻翼基底切除术 +缝合法鼻翼沟成形术 ;C类方法 :鼻尖鼻翼浅筋膜切除术 +纤维肌层切除术 ,Ⅱ期行鼻翼基底切除术 +缝合法鼻翼沟成形术。结果 鼻下端软组织各层微血管密度从大到小的顺序为软骨膜层、网状层、纤维肌层、乳头下层、浅筋膜层、乳头层。本组 18例鼻下端圆隆肥大患者术后效果优良 ,无严重并发症 ,但肿胀时间略延长。结论 ①鼻端皮瓣减薄术不致引起鼻端部皮肤血运严重障碍。②A ,B 。

鼻下端圆隆肥大的鼻成形术治疗

目的:探讨鼻下端圆隆肥大的整形手术方法。方法:选取2011年1月～2012年8月来我美容院进行整形手术的鼻下端圆隆肥大患者148例,随机分为对照组和观察组,每组各74例,对照组采用鼻翼软骨缝合术+鼻翼沟成形术+硅胶假体和耳廓软骨隆鼻+耳廓软骨游离移植鼻尖综合手术,观察组采用鼻翼沟形成术+鼻中隔软骨切取翻转与移植+鼻翼软骨固定缝合术综合疗法,随访6～18个月,观察两组的术后效果、并发症及满意度。结果:经过治疗术后效果均良好,观察组的满意度为90.5%(67/74),对照组的满意度为93.2%(69/74),对照组略高于观察组,但经统计学处理,发现两组满意度无统计学差异(P>0.05)。观察组和对照组并发症的发生率分别为5.4%和8.1%,两组间并发症发生率也无统计学差异(P>0.05)。结论:鼻下端圆隆肥大可经过合理有效的综合疗法,均可达到良好的美容整形效果,值得临床推广应用。

Preparation and Identification of Specific Monoclonal Antibody against Porcine Circovirus Type 2

BALB/c mice were immunized using synthetic tandem polypeptide of Cap protein epitope of porcine circovirus type 2 （PCV2） as the antigen. By using lym-phocyte hybridoma technique, a hybridoma cellline stably secreting monoclonal an-tibody against PCV2-rCap protein was successful y obtained and named as 670#. The ascites titer of the obtained monoclonal antibody was 1∶100 000. Western blot results showed that the monoclonal antibody could react with prokaryotical y ex-pressed PET32a-ORF2 recombinant protein, eukaryotical y expressed ORF1-ORF2 tandem protein and PCV2 whole virus celllysate. Indirect EILSA demonstrated that the monoclonal antibody could bind with ORF1-ORF2 tandem protein. Indirect im-munofluorescence assay （IFA） indicated that the monoclonal antibody could identify native PCV2 virus. The preparation of this monoclonal antibody provided technical tools for epitope analysis and molecular diagnosis of PCV2 virus.

The Establishment of Immunochemistry Test Based on a Synthetic Peptide Antibody for the Detection of a Porcine Circovirus-Like Virus P1

Recently, a novel porcine circovirus-like virus P1 with a circular DNA genome of 0.648 kb was identified. P1 antigen was detected both in vitro and in vivo by synthetic peptide-derived polyclonal antibody-based immunochemistry. The designed peptides were synthesized by solid-phase technique, purified by high performance liquid chromatography, coupled to Keyhole limpet hemocyanin, and injected into rabbits to prepare polyclonal antibody. The emergence of positive cells revealed that synthetic peptide could elicit antibodies against P1 and viral protein could be synthesized. The polyclonal peptide antibodies described here was successfully applied to immunochemical staining and proved helpful in diagnosing P1.

北阡新石器时代人类颅内模

一、前言颅内模(endocast)是指填充整个颅骨内部空腔获得的颅腔铸型,包括天然形成的'化石脑'、人工复原(石膏等填充)以及经过CT技术复原的颅内模[1]。通过对颅内模的观察、测量分析,间接地反映了脑的表面形态特征、脑量等信息,颅内模因此成为分析和探讨人类脑进化的重要研究材料。通过对颅内模的大体形态观察,古人类学家探索了人类进化过程中脑的演化历程:脑量的大小随地质时代变化存在增加的趋势[2],随着脑量的增大,额叶趋向圆隆。