土人参根健脾益气功效及其作用机制的实验研究

目的:探讨土人参根的健脾益气功效和作用机制。方法:应用大黄泻下结合定期禁食方法,复制脾气虚动物模型,观察土人参根对脾虚大鼠模型生理功能,GAS、MOT,细胞因子IgM、IgG、C3、C4的影响。结果:土人参根能促进脾虚大鼠机体生长发育,提高GAS、MOT、IgM、IgG、C3、C4水平。结论:土人参根有显著的健脾益气作用,其作用机制与提高胃动素、胃泌素、IgM、IgG、C3、C4等水平有关。

土人参根多糖提取及含量测定

目的:提取土人参根的多糖并进行含量测定。方法:采用水提醇沉法提取土人参根多糖,以葡萄糖标准液绘制标准曲线,用苯酚—硫酸法,分光光度法在484 nm波长处测定土人参根多糖的含量。结果:回归方程为y=7.4625x-0.0484,相关系数r=0.9999,平均回收率98.9%,RSD=0.31%(n=5)。结论:土人参根多糖含量为3.7%,方法操作简便、重现性好、准确、可靠,可作为检测土人参根多糖含量的优选方法。

Dynamic of Soil Microorganisms from Root Region of Ginseng with Different Growing Years

Objective To see the dynamic of fungi, bacilli and actinomyces communities from root region of ginseng with different growing years.Method With ginseng root region soils from several sampling sites of Jilin Province as materials, concentrations of fungi, bacilli and antinomyces were evaluated by spread-plate method. Result Though there are differences on statistic data among soil samples, commonly with the increasing of growing years, concentration of fungi in ginseng root region increased, which were on the contrary for bacilli and antinomyces, and bacilli changed even more significant than antinomyces. Conclusion Concentrations of soil microorganisms can be influenced by soil type, planting mode and growing years simultaneously, but growing years influenced even more significantly.