Forty-five rhizofungal isolates were isolated, identified and characterized from 11 herbicides polluted-soil. Among the isolates, 10 fungal species proved to be the most potent and promising ones in herbicides toleran...Forty-five rhizofungal isolates were isolated, identified and characterized from 11 herbicides polluted-soil. Among the isolates, 10 fungal species proved to be the most potent and promising ones in herbicides tolerance. The herbicides exhibited severe and dramatic effect and modulation on fungal DNA and protein represented in DNA and protein profile. Severely loss in the total soluble cell ions （SCI） and total cell protein percentage （TCPC） concentrations were observed. The loss of SCI by glyphosate, Aspergillus flavus （86,30%） was the most affected one, followed by Penicillium spiculisporus （76,30%）, Penicilliurn verruculosum （64.40%） and Alternaria tenuissima （64%）, respectively. For pendimethalin, Alternaria tenuissima （54.01%） was the most affected fungi. For diclofop-methyl, Penicillium spiculisporus （74.20%） was the most affected fungi. The loss of TCPC by glyphosate, Alternaria tenuissima （64.71%） was the most effected fungi, followed by Penicillium spiculisporus （57.14%）, respectively. For pendimethalin, A. terreus （54.29%） w, as the most affected fungi. For diclofop-methyl, Penicillium spiculisporus （60%） was the most affected fungi, fbllowed by Alternaria tenuissima （58.82%）, Aspergillus tamarii （55.56%）, respectively. The results proved severe reductions and alteration in protein, SCI, TCPC and DNA in fungal strains exposed to these herbicides which might reflex a degree of tolerance occurred during the assimilation of those toxic compounds from the pesticides polluted-soil.展开更多
In Kazakhstan, there are obsolete pesticides which should be buried in special dedicated and prepared burial sites or neutralized for minimization of dangerous genetic and ecological risks. The work in two years was i...In Kazakhstan, there are obsolete pesticides which should be buried in special dedicated and prepared burial sites or neutralized for minimization of dangerous genetic and ecological risks. The work in two years was identifying the obsolete pesticides in 91 the former warehouses of chemical plant protection products. In two regions （Almaty and Akmola regions） of Kazakhstan, the total amount of 388.6 tones of obsolete pesticides and unidentified stockpile material was observed, along with 513 pesticide containers. Results of the study have shown that soil around storehouses polluted by organochlorine pesticides residues in soil included metabolites of DDT （p, p＇-dichlorodiphenyltrichloroethane） and HCH （hexachlorocyclohexane）. The strategy was to identify pesticide-tolerant plant genotypes which can be used for phytoremediation of pesticide-contaminated soils in Almaty regions of Kazakhstan. The results have shown that selected wild species plants demonstrated ability to phytoaccumulation of organochlorine metabolites and reduce pesticide concentrations in soil （control without plant）. It was shown that accumulation of pesticides depended on the species of plants, plant biomass and pesticide concentrations in soil. The use of mineral fertilizers resulted in stimulation of growth and biomass accumulation followed by an increasing phytoextraction.展开更多
Urban soil is the main component of urban ecological systems and the key risk receptor from urbanization. Heavy metal and green pesticide pollutions in urban soils have been widely reported with the expanding of urban...Urban soil is the main component of urban ecological systems and the key risk receptor from urbanization. Heavy metal and green pesticide pollutions in urban soils have been widely reported with the expanding of urbanization. Since urban soil pollution comes from various resources, application of integrated thinking and methods is needed in ecological risk assessment of urban soil pollution. This paper synthetically reviewed the combined pollution of heavy metals and pesticide, and ecological risk assessment, and then proposed some research trends and areas in the future that are required to carry out intensively according to the present situation of environmental pollution and international research fronts.展开更多
Organophosphorus pesticides （OPs） are one of the most regular pollutants and frequently detected in the contaminated sites, so developing an efficient method for the treatment of OPs is highly required. The aim of t...Organophosphorus pesticides （OPs） are one of the most regular pollutants and frequently detected in the contaminated sites, so developing an efficient method for the treatment of OPs is highly required. The aim of the present study was to compare the effectiveness of persulfate （PS） activation and Fenton reaction in remediating the soil polluted with OPs. The polluted soil used in this study was sampled from an abandoned insecticide factory in Nantong, Jiangsu Province of China, mainly containing chloropyrifos （CP） and 4-bromo-2-chlorophenol （BCP, the raw material of profenofos） with total concentration of about 30 000 mg kg- 1. The results showed that both BCP and CP were efficiently degraded by base activation of PS, and increasing the ratio of NaOH/PS enhanced CP degradation, but slightly decreased BCP degradation. The greatest degradation rates for CP and BCP were 92% and 97%, respectively, with 7.0 tool L-1 NaOH and 0.21 tool L-1 PS and a soil-to-liquid ratio of 1：1. Furthermore, ferrous iron activation of PS also degraded BCP efficiently, but only 60% of CP was degraded under the same reaction conditions. These results indicated that base activation of PS was more feasible than Fe2＋ activation and Fenton reaction in remediating the soil polluted with OPs. The high degradation rate for CP may be linked to the initial hydrolyzation of CP by base to 3,5,6-trichloro-2-pyridinol, which can be further rapidly degraded by free radicals generated from base activation of PS.展开更多
Problems associated with organochlorine pesticide （OCP）-contaminated sites in China have received wide attention. To solve such problems, innovative ex-situ methods of site remediation are urgently needed. We invest...Problems associated with organochlorine pesticide （OCP）-contaminated sites in China have received wide attention. To solve such problems, innovative ex-situ methods of site remediation are urgently needed. We investigated the feasibility of the extraction method with different organic solvents, ethanol, 1-propanol, and three fractions of petroleum ether, using a soil collected from Wujiang （W J）, China, a region with long-term contamination of dichlorodiphenyltrichloroethanes （DDTs）. We evaluated different influential factors, including organic solvent concentration, washing time, mixing speed, solutiomto-soil ratio, and washing temperature, on the removal of DDTs from the WJ soil. A set of relatively better parameters were selected for extraction with 100 mL L-1 petroleum ether （60-90 ℃）： washing time of 180 min, mixing speed of 100 r min-I, solution-to-soil ratio of 10：1, and washing temperature of 50 ℃. These selected parameters were also applied on three other seriously OCP-polluted soils. Results demonstrated their broad-spectrum effectiveness and excellent OCP extraction performance on the contaminated soils with different characteristics.展开更多
Chlordecone, one of the most persistent organochlorine pesticides, was applied between 1972 and 1993 in banana fields in the French West Indies, which results in long-term pollution of soils and contamination of water...Chlordecone, one of the most persistent organochlorine pesticides, was applied between 1972 and 1993 in banana fields in the French West Indies, which results in long-term pollution of soils and contamination of waters, aquatic biota, and crops. As human exposure to chlordecone is mainly due to food contamination, early research was focused on chlordecone transfer to crops. Field trials were conducted to investigate chlordecone contamination of yam, sweet potato, turnip, and radish grown on a Ferralic Nitisol polluted by chlordecone. We also carried out trials on yam, courgette, and tomato under greenhouse conditions with homogenized Andosol and Nitisol, polluted by chlordecone to various extents. Our results indicated that i) all tubers were contaminated in accordance with the chlordecone content of the soils; ii) the plant contamination capacity of the Nitisol was greater than that of the Andosol; and iii) whatever the soil type, tuber contamination was related to the soil volumetric content of dissolved chlordecone. Nevertheless, no tubers showed sufficient chlordecone uptake for efficient soil decontamination by means of plant extraction. Soil contact accounted for most of the root crop contamination, which was inversely proportional to the tuber size. Internal transfer might also increase root crop contamination when the root central cylinder contained raw sap flow, as in the case of turnip or radish. Courgette fruits showed high contamination without soil contact. Thus, further research is needed to explore the pattern of both below- and aboveground plant chlordecone contamination and assess the hypothesis of its correlation with sap flow. Finally, we used our results to build a decisionmaking tool for farmers, relating soil pollution with the maximal contamination of the harvested organs to predict crop contamination and thus assisting farmers in making crop choices at planting in order to conform with the European Union's regulations.展开更多
文摘Forty-five rhizofungal isolates were isolated, identified and characterized from 11 herbicides polluted-soil. Among the isolates, 10 fungal species proved to be the most potent and promising ones in herbicides tolerance. The herbicides exhibited severe and dramatic effect and modulation on fungal DNA and protein represented in DNA and protein profile. Severely loss in the total soluble cell ions （SCI） and total cell protein percentage （TCPC） concentrations were observed. The loss of SCI by glyphosate, Aspergillus flavus （86,30%） was the most affected one, followed by Penicillium spiculisporus （76,30%）, Penicilliurn verruculosum （64.40%） and Alternaria tenuissima （64%）, respectively. For pendimethalin, Alternaria tenuissima （54.01%） was the most affected fungi. For diclofop-methyl, Penicillium spiculisporus （74.20%） was the most affected fungi. The loss of TCPC by glyphosate, Alternaria tenuissima （64.71%） was the most effected fungi, followed by Penicillium spiculisporus （57.14%）, respectively. For pendimethalin, A. terreus （54.29%） w, as the most affected fungi. For diclofop-methyl, Penicillium spiculisporus （60%） was the most affected fungi, fbllowed by Alternaria tenuissima （58.82%）, Aspergillus tamarii （55.56%）, respectively. The results proved severe reductions and alteration in protein, SCI, TCPC and DNA in fungal strains exposed to these herbicides which might reflex a degree of tolerance occurred during the assimilation of those toxic compounds from the pesticides polluted-soil.
文摘In Kazakhstan, there are obsolete pesticides which should be buried in special dedicated and prepared burial sites or neutralized for minimization of dangerous genetic and ecological risks. The work in two years was identifying the obsolete pesticides in 91 the former warehouses of chemical plant protection products. In two regions （Almaty and Akmola regions） of Kazakhstan, the total amount of 388.6 tones of obsolete pesticides and unidentified stockpile material was observed, along with 513 pesticide containers. Results of the study have shown that soil around storehouses polluted by organochlorine pesticides residues in soil included metabolites of DDT （p, p＇-dichlorodiphenyltrichloroethane） and HCH （hexachlorocyclohexane）. The strategy was to identify pesticide-tolerant plant genotypes which can be used for phytoremediation of pesticide-contaminated soils in Almaty regions of Kazakhstan. The results have shown that selected wild species plants demonstrated ability to phytoaccumulation of organochlorine metabolites and reduce pesticide concentrations in soil （control without plant）. It was shown that accumulation of pesticides depended on the species of plants, plant biomass and pesticide concentrations in soil. The use of mineral fertilizers resulted in stimulation of growth and biomass accumulation followed by an increasing phytoextraction.
文摘Urban soil is the main component of urban ecological systems and the key risk receptor from urbanization. Heavy metal and green pesticide pollutions in urban soils have been widely reported with the expanding of urbanization. Since urban soil pollution comes from various resources, application of integrated thinking and methods is needed in ecological risk assessment of urban soil pollution. This paper synthetically reviewed the combined pollution of heavy metals and pesticide, and ecological risk assessment, and then proposed some research trends and areas in the future that are required to carry out intensively according to the present situation of environmental pollution and international research fronts.
基金supported by the National Key Basic Research Program of China (No. 2013CB934303)the Environmental Protection Program of Jiangsu Province of China (No. 2015011)the Youth Innovation Promotion Association of Chinese Academy of Sciences (No. 2014270)
文摘Organophosphorus pesticides （OPs） are one of the most regular pollutants and frequently detected in the contaminated sites, so developing an efficient method for the treatment of OPs is highly required. The aim of the present study was to compare the effectiveness of persulfate （PS） activation and Fenton reaction in remediating the soil polluted with OPs. The polluted soil used in this study was sampled from an abandoned insecticide factory in Nantong, Jiangsu Province of China, mainly containing chloropyrifos （CP） and 4-bromo-2-chlorophenol （BCP, the raw material of profenofos） with total concentration of about 30 000 mg kg- 1. The results showed that both BCP and CP were efficiently degraded by base activation of PS, and increasing the ratio of NaOH/PS enhanced CP degradation, but slightly decreased BCP degradation. The greatest degradation rates for CP and BCP were 92% and 97%, respectively, with 7.0 tool L-1 NaOH and 0.21 tool L-1 PS and a soil-to-liquid ratio of 1：1. Furthermore, ferrous iron activation of PS also degraded BCP efficiently, but only 60% of CP was degraded under the same reaction conditions. These results indicated that base activation of PS was more feasible than Fe2＋ activation and Fenton reaction in remediating the soil polluted with OPs. The high degradation rate for CP may be linked to the initial hydrolyzation of CP by base to 3,5,6-trichloro-2-pyridinol, which can be further rapidly degraded by free radicals generated from base activation of PS.
基金Supported by the National High Technology Research and Development Program of China (No. 2009AA063103)the National Natural Science Foundation of China (No. 41030531)
文摘Problems associated with organochlorine pesticide （OCP）-contaminated sites in China have received wide attention. To solve such problems, innovative ex-situ methods of site remediation are urgently needed. We investigated the feasibility of the extraction method with different organic solvents, ethanol, 1-propanol, and three fractions of petroleum ether, using a soil collected from Wujiang （W J）, China, a region with long-term contamination of dichlorodiphenyltrichloroethanes （DDTs）. We evaluated different influential factors, including organic solvent concentration, washing time, mixing speed, solutiomto-soil ratio, and washing temperature, on the removal of DDTs from the WJ soil. A set of relatively better parameters were selected for extraction with 100 mL L-1 petroleum ether （60-90 ℃）： washing time of 180 min, mixing speed of 100 r min-I, solution-to-soil ratio of 10：1, and washing temperature of 50 ℃. These selected parameters were also applied on three other seriously OCP-polluted soils. Results demonstrated their broad-spectrum effectiveness and excellent OCP extraction performance on the contaminated soils with different characteristics.
基金Supported by the Ministry of Ecology and Sustainable Development and the Ministry of Agriculture and Food,France,the French National Agency of Research,and the French National Chlordecone Action Plan
文摘Chlordecone, one of the most persistent organochlorine pesticides, was applied between 1972 and 1993 in banana fields in the French West Indies, which results in long-term pollution of soils and contamination of waters, aquatic biota, and crops. As human exposure to chlordecone is mainly due to food contamination, early research was focused on chlordecone transfer to crops. Field trials were conducted to investigate chlordecone contamination of yam, sweet potato, turnip, and radish grown on a Ferralic Nitisol polluted by chlordecone. We also carried out trials on yam, courgette, and tomato under greenhouse conditions with homogenized Andosol and Nitisol, polluted by chlordecone to various extents. Our results indicated that i) all tubers were contaminated in accordance with the chlordecone content of the soils; ii) the plant contamination capacity of the Nitisol was greater than that of the Andosol; and iii) whatever the soil type, tuber contamination was related to the soil volumetric content of dissolved chlordecone. Nevertheless, no tubers showed sufficient chlordecone uptake for efficient soil decontamination by means of plant extraction. Soil contact accounted for most of the root crop contamination, which was inversely proportional to the tuber size. Internal transfer might also increase root crop contamination when the root central cylinder contained raw sap flow, as in the case of turnip or radish. Courgette fruits showed high contamination without soil contact. Thus, further research is needed to explore the pattern of both below- and aboveground plant chlordecone contamination and assess the hypothesis of its correlation with sap flow. Finally, we used our results to build a decisionmaking tool for farmers, relating soil pollution with the maximal contamination of the harvested organs to predict crop contamination and thus assisting farmers in making crop choices at planting in order to conform with the European Union's regulations.
