A species-specific PCR assay was established for rapid and accurate detection of the oomycete pathogen Phytophthora tentaculata in diseased plant tissues and infected soil.A pair of species-specific primers Pt1/Pt2 we...A species-specific PCR assay was established for rapid and accurate detection of the oomycete pathogen Phytophthora tentaculata in diseased plant tissues and infected soil.A pair of species-specific primers Pt1/Pt2 were designed on the basis of Ras-related protein(Ypt1) gene sequences of the Phytophthora species.PCR amplification with the Pt primers resulted in a 386 bp product only from isolates of P.tentaculata.The detection threshold with Pt primers was 100 pg of genomic DNA.A nested PCR procedure was developed using Ypt1F/Ypt1R as the first-round amplification primers and Pt1/Pt2 as the second-round primers,which increased the detection sensitivity 100-fold to 1 pg.PCR using these Pt primers can also be used to detect P.tentaculata in naturally infected plant tissues and soil.The PCR-based method developed in this study provides a rapid and sensitive tool for detection of P.tentaculata.展开更多
The geographic distribution of Gordonia acuminata in four provinces of Yunnan,Guizhou,Sichuan and Guangxi is disconnected. The whole distribution region is divided into three parts: Sichuan(A), Guangxi(B), Yunnan(C). ...The geographic distribution of Gordonia acuminata in four provinces of Yunnan,Guizhou,Sichuan and Guangxi is disconnected. The whole distribution region is divided into three parts: Sichuan(A), Guangxi(B), Yunnan(C). The Kira’ WI and CI indices and the Xu wenduo’ index were used to analyze the water and thermal range of the species distribution. The adaptive range of G. acuminata to light, soil type and pH was also discussed.展开更多
Molecular Soil Biology (ISSN 1925-2005), an open access, peer reviewed journal, publishing original contribution related to the soil biology at the molecular level. All manuscripts
基金The Key Project for Breeding Genetically Modified Organisms(Grant No.2011ZX08012-005)
文摘A species-specific PCR assay was established for rapid and accurate detection of the oomycete pathogen Phytophthora tentaculata in diseased plant tissues and infected soil.A pair of species-specific primers Pt1/Pt2 were designed on the basis of Ras-related protein(Ypt1) gene sequences of the Phytophthora species.PCR amplification with the Pt primers resulted in a 386 bp product only from isolates of P.tentaculata.The detection threshold with Pt primers was 100 pg of genomic DNA.A nested PCR procedure was developed using Ypt1F/Ypt1R as the first-round amplification primers and Pt1/Pt2 as the second-round primers,which increased the detection sensitivity 100-fold to 1 pg.PCR using these Pt primers can also be used to detect P.tentaculata in naturally infected plant tissues and soil.The PCR-based method developed in this study provides a rapid and sensitive tool for detection of P.tentaculata.
文摘The geographic distribution of Gordonia acuminata in four provinces of Yunnan,Guizhou,Sichuan and Guangxi is disconnected. The whole distribution region is divided into three parts: Sichuan(A), Guangxi(B), Yunnan(C). The Kira’ WI and CI indices and the Xu wenduo’ index were used to analyze the water and thermal range of the species distribution. The adaptive range of G. acuminata to light, soil type and pH was also discussed.
文摘Molecular Soil Biology (ISSN 1925-2005), an open access, peer reviewed journal, publishing original contribution related to the soil biology at the molecular level. All manuscripts
