Effects of temperature and drying and wetting alternation(DWA)on ammonium fixation in manured loessial soil were studied by means of Batch Equilibrium with Varying concentration solutions of ammonium chloride,ammonium...Effects of temperature and drying and wetting alternation(DWA)on ammonium fixation in manured loessial soil were studied by means of Batch Equilibrium with Varying concentration solutions of ammonium chloride,ammonium fixation time,and soil clay contents.The purpose of the research was to find out the pattern of ammonium fixation ted by the varying factors.The results showed a remarkable variation in ammonium fixation.Fixed ammonium increased with temperature and treatments of DWA.The ammonium fixation in manured loessial soil was characterized by the effect of temperature and DWA.展开更多
The effect of ammonium fixation on the estimation of soil microbial biomass N was studied bv thestandard fumigation-incubation (FI) and fumigation-extraction (FE) methods. NO_3-N content of fumigatedsoil changed littl...The effect of ammonium fixation on the estimation of soil microbial biomass N was studied bv thestandard fumigation-incubation (FI) and fumigation-extraction (FE) methods. NO_3-N content of fumigatedsoil changed little during incubation, while the fixed NH in soils capable of fixing NH increased withthe increase of K_2SO_4-extractable NH_4-N. One day fumigation increased both extractable NH and fixedNH. However, prolonged fumigation gave no further increase. One day fumigation caused significant loss ofNO_3-N, while prolonged fumigation caused no further loss. For soils tested, the net increases of fixed NHin fumigated soil equaled to 0-94% of NH_4-N flush measured by the FI method, and 1-74% of extractable Nmeasured by the FE method, depending on different soils. It is concluded that the ammonium fixation wasone of the processes taking place in soils during fumigation as well as incubation after fumigation and shouldnot be neglected in the estimation of microbial biomass nitrogen by either FI or FE method.展开更多
To study effect of C2H2 and change of headspace gas on N2O emission, denitrification, as well as CO2emission, slumes of an agricultural soil were anaerobically incubated for 7 da3’s at 25 ℃. Both N2O reduction and C...To study effect of C2H2 and change of headspace gas on N2O emission, denitrification, as well as CO2emission, slumes of an agricultural soil were anaerobically incubated for 7 da3’s at 25 ℃. Both N2O reduction and CO2 emissions were inhibited by the addition of 100 mL L-1 of C2H2. However, the inhibition to CO2 emission was alleviated by the replacement of headspace gas, and the N2O emission was enhanced by the replacement. Acetylene disappeared evidently from the soil slumes during the incubation. Consequently results obtained from the traditional C2H2 blocking technique for determination of denitrification rate, especially in a long-time incubation, should be explained with care because of its side effect existing in the incubation environments without change of headspace gas. To reduce the possible side effect on the processes other than denitrification, it is suggested that headspace gas should be replaced several times during a long-time incubation.展开更多
The partitioning of nitrogen deposition among forest soil （including forest floor）, leachate and above- and belowground biomass of pot cultured beech seedlings in comparison to non-cultured treatments were investiga...The partitioning of nitrogen deposition among forest soil （including forest floor）, leachate and above- and belowground biomass of pot cultured beech seedlings in comparison to non-cultured treatments were investigated by adding 1.92 g.m^-2 ^15N tracer in throughfall for two successive growing seasons at a greenhouse experiment. Ammonium and nitrate depositions were simulated on four treatments （cultured and non-cultured） and each treatment was labeled with either ^15N-NH4^＋ or ^15N-NO3^-. Total recovery rates of the applied ^15N in the whole system accounted for 74.9% to 67.3% after ^15N-NH4^＋ and 85.3% to 88.1% after ^15N-NO3^-in cultured and non-cultured treatments, respectively. The main sink for both ^15N tracers was the forest soil （including forest floor）, where 34.6% to 33.7% of ^15N-NH4^＋ and 13.1% to 9.0% of ^15N-NO3^-were found in cultured and non-cultured treatments, respectively, suggesting strong immobilization of both N forms by hetero- trophic microorganisms. Nitrogen immobilization by microorganisms in the forest soil （including forest floor） was three times higher when ^15N-NH4^＋ was applied compared to ^15N-NO3^-. The preferential heterotrophic use of ammonium resulted in a two times higher retention of deposited ^15N-NH4^＋ in the forest soil as compared to plants. In contrast, nitrate immobilization in the forest soil was lower compared to plants, although statistically it was not significantly different. In total the immobilization of ammonium in the plant-soil system was about 60% higher than nitrate, indicating the importance of the N-forms deposition for retention in forest ecosystems.展开更多
Ninety-three soil samples and 19 sedimentary rock samples collected from 21 provinces of China wereanalyzed for their contents of fixed ammonium and total N by Kjeldahl-HF method. Results showed thatamount of difficul...Ninety-three soil samples and 19 sedimentary rock samples collected from 21 provinces of China wereanalyzed for their contents of fixed ammonium and total N by Kjeldahl-HF method. Results showed thatamount of difficultly extractable fixed ammonium (the fixed ammonium that is not determinable by Kjeldahlprocedures commonly used for soils) in soils ranged from 0 to 202 mg kg--1. It was generally more than50 mg kg--1 in soils in Chanaii and Thrpan districts, Xinjiang, accounting for 3.2%~36.8% with an averageof 13.9% of the total N. For some Orthents derived from purple shale and purple sandstone in Sichuan andHunan provinces and Chao soils derived from secondary loess in Henan Province and Ningxia AutonomousRegion it was generally around 30 mg kg--1 , accounting for 4%~7% of the total soil N, and for most of therest of soils studied, with the exception of some subsoils, no or trace difficultly extractable fixed ammoniumcould be detected. It was suggested that the difficultly extractable fixed ammonium was originated fromParent rock, and for slightly weathered soils derived from parent materials rich in this form of N the Kjeldahlmethod might give underestimation of total soil N.展开更多
Dynamics of fixed NH in NH-treated soils incubated with glucose at 37±2℃ during the course ofincubation and factors affecting it were studied. Results showed that content of fixed NH in soil reached aminimun on ...Dynamics of fixed NH in NH-treated soils incubated with glucose at 37±2℃ during the course ofincubation and factors affecting it were studied. Results showed that content of fixed NH in soil reached aminimun on day 7 after incubation and then increased gradually regardless of the amount of glucose addedand the kind of soil tested. However, the amount of fixed NH released from the soil at the given time variedwith both the amount of glucose added and the kind of soil examined. In cases glucose was added at a rateof 10.0g C/kg soil, the amount of fixed NH retained in soil after 7 days of incubation was almost identicalto that found by Neubauer test. Addition of K￣+ depressed the release of fixed NH significantly. Based onthe results obtained a method for determining the content of available fixed NH in soils was proposed andthe amount of N as available fixed NH in two soils measured by this method on an area profile-depth basiswas presented.展开更多
Dear Editor,The nitrogen-fixing symbiotic bacteria, rhizobia are the most important beneficial bacteria in soil, as they form nodules with host legume plants to fix nitrogen to maintain soil fertility and facilitate p...Dear Editor,The nitrogen-fixing symbiotic bacteria, rhizobia are the most important beneficial bacteria in soil, as they form nodules with host legume plants to fix nitrogen to maintain soil fertility and facilitate plant growth. Although these bacteria are critically important to agriculture, bacteriophages of rhizobia (rhizobiophages) are commonly present in soils, rhizosphere,and nodules, which have major influences on the composition and population of rhizobia in soils and affect nodulation and nitrogen fixation. Despite the importance of rhizobiophages,only 23 phage full genomes have been sequenced.展开更多
文摘Effects of temperature and drying and wetting alternation(DWA)on ammonium fixation in manured loessial soil were studied by means of Batch Equilibrium with Varying concentration solutions of ammonium chloride,ammonium fixation time,and soil clay contents.The purpose of the research was to find out the pattern of ammonium fixation ted by the varying factors.The results showed a remarkable variation in ammonium fixation.Fixed ammonium increased with temperature and treatments of DWA.The ammonium fixation in manured loessial soil was characterized by the effect of temperature and DWA.
文摘The effect of ammonium fixation on the estimation of soil microbial biomass N was studied bv thestandard fumigation-incubation (FI) and fumigation-extraction (FE) methods. NO_3-N content of fumigatedsoil changed little during incubation, while the fixed NH in soils capable of fixing NH increased withthe increase of K_2SO_4-extractable NH_4-N. One day fumigation increased both extractable NH and fixedNH. However, prolonged fumigation gave no further increase. One day fumigation caused significant loss ofNO_3-N, while prolonged fumigation caused no further loss. For soils tested, the net increases of fixed NHin fumigated soil equaled to 0-94% of NH_4-N flush measured by the FI method, and 1-74% of extractable Nmeasured by the FE method, depending on different soils. It is concluded that the ammonium fixation wasone of the processes taking place in soils during fumigation as well as incubation after fumigation and shouldnot be neglected in the estimation of microbial biomass nitrogen by either FI or FE method.
文摘To study effect of C2H2 and change of headspace gas on N2O emission, denitrification, as well as CO2emission, slumes of an agricultural soil were anaerobically incubated for 7 da3’s at 25 ℃. Both N2O reduction and CO2 emissions were inhibited by the addition of 100 mL L-1 of C2H2. However, the inhibition to CO2 emission was alleviated by the replacement of headspace gas, and the N2O emission was enhanced by the replacement. Acetylene disappeared evidently from the soil slumes during the incubation. Consequently results obtained from the traditional C2H2 blocking technique for determination of denitrification rate, especially in a long-time incubation, should be explained with care because of its side effect existing in the incubation environments without change of headspace gas. To reduce the possible side effect on the processes other than denitrification, it is suggested that headspace gas should be replaced several times during a long-time incubation.
基金supported by ibw, Goet-tingen University, Germany
文摘The partitioning of nitrogen deposition among forest soil （including forest floor）, leachate and above- and belowground biomass of pot cultured beech seedlings in comparison to non-cultured treatments were investigated by adding 1.92 g.m^-2 ^15N tracer in throughfall for two successive growing seasons at a greenhouse experiment. Ammonium and nitrate depositions were simulated on four treatments （cultured and non-cultured） and each treatment was labeled with either ^15N-NH4^＋ or ^15N-NO3^-. Total recovery rates of the applied ^15N in the whole system accounted for 74.9% to 67.3% after ^15N-NH4^＋ and 85.3% to 88.1% after ^15N-NO3^-in cultured and non-cultured treatments, respectively. The main sink for both ^15N tracers was the forest soil （including forest floor）, where 34.6% to 33.7% of ^15N-NH4^＋ and 13.1% to 9.0% of ^15N-NO3^-were found in cultured and non-cultured treatments, respectively, suggesting strong immobilization of both N forms by hetero- trophic microorganisms. Nitrogen immobilization by microorganisms in the forest soil （including forest floor） was three times higher when ^15N-NH4^＋ was applied compared to ^15N-NO3^-. The preferential heterotrophic use of ammonium resulted in a two times higher retention of deposited ^15N-NH4^＋ in the forest soil as compared to plants. In contrast, nitrate immobilization in the forest soil was lower compared to plants, although statistically it was not significantly different. In total the immobilization of ammonium in the plant-soil system was about 60% higher than nitrate, indicating the importance of the N-forms deposition for retention in forest ecosystems.
文摘Ninety-three soil samples and 19 sedimentary rock samples collected from 21 provinces of China wereanalyzed for their contents of fixed ammonium and total N by Kjeldahl-HF method. Results showed thatamount of difficultly extractable fixed ammonium (the fixed ammonium that is not determinable by Kjeldahlprocedures commonly used for soils) in soils ranged from 0 to 202 mg kg--1. It was generally more than50 mg kg--1 in soils in Chanaii and Thrpan districts, Xinjiang, accounting for 3.2%~36.8% with an averageof 13.9% of the total N. For some Orthents derived from purple shale and purple sandstone in Sichuan andHunan provinces and Chao soils derived from secondary loess in Henan Province and Ningxia AutonomousRegion it was generally around 30 mg kg--1 , accounting for 4%~7% of the total soil N, and for most of therest of soils studied, with the exception of some subsoils, no or trace difficultly extractable fixed ammoniumcould be detected. It was suggested that the difficultly extractable fixed ammonium was originated fromParent rock, and for slightly weathered soils derived from parent materials rich in this form of N the Kjeldahlmethod might give underestimation of total soil N.
文摘Dynamics of fixed NH in NH-treated soils incubated with glucose at 37±2℃ during the course ofincubation and factors affecting it were studied. Results showed that content of fixed NH in soil reached aminimun on day 7 after incubation and then increased gradually regardless of the amount of glucose addedand the kind of soil tested. However, the amount of fixed NH released from the soil at the given time variedwith both the amount of glucose added and the kind of soil examined. In cases glucose was added at a rateof 10.0g C/kg soil, the amount of fixed NH retained in soil after 7 days of incubation was almost identicalto that found by Neubauer test. Addition of K￣+ depressed the release of fixed NH significantly. Based onthe results obtained a method for determining the content of available fixed NH in soils was proposed andthe amount of N as available fixed NH in two soils measured by this method on an area profile-depth basiswas presented.
基金supported by the Strategic Priority Research Program of Chinese Academy of Sciences (XDB15010103)National Natural Science Foundation of China (41301259, 41671251)Youth Innovation Promotion Association CAS
文摘Dear Editor,The nitrogen-fixing symbiotic bacteria, rhizobia are the most important beneficial bacteria in soil, as they form nodules with host legume plants to fix nitrogen to maintain soil fertility and facilitate plant growth. Although these bacteria are critically important to agriculture, bacteriophages of rhizobia (rhizobiophages) are commonly present in soils, rhizosphere,and nodules, which have major influences on the composition and population of rhizobia in soils and affect nodulation and nitrogen fixation. Despite the importance of rhizobiophages,only 23 phage full genomes have been sequenced.
