模拟氮沉降对木荷人工幼林地土壤氮素、碳素和微生物量垂直分布的影响

以NH4NO3作为氮源,对广州东北郊木荷（Schima superba）人工幼林地进行模拟氮沉降处理,共设置3个氮沉降水平,分别为N0（N：0 g.m-2.a-1）、N5（N：5 g.m-2.a-1）以及N10（N：10 g.m-2.a-1）,每月进行喷施。在连续施氮22个月（当月当次施氮5天后）对土壤氮素（硝氮、氨氮、总氮）、碳素（总碳）以及微生物量（脂磷）在0~60 cm土层中的垂直分布进行研究。结果显示：在3个氮沉降水平下,随着土层加深,pH呈现出下降的趋势,氮沉降存在加剧土壤酸化的风险;在N0、N5、N10水平下,土壤全氮和总碳的垂直分布趋势大体一致,随着土层加深,其含量下降,但在深层土壤（40~60 cm）中,施氮与对照比较,总碳呈现一定的增加趋势;除40~50 cm土层,N5、N10水平下的硝态氮含量在各个深度土壤中都表现为比对照组要高,氮沉降导致土壤一定程度上硝态氮的积累;在浅层土壤（0~20 cm）中,铵态氮水平较低并且其含量明显低于对照组,而在较深的土层中铵态氮有较多的积累,说明存在污染地下水的风险;N5和N10水平下,无机氮比例（无机氮含量与总氮含量之比）在各个深度土壤中总体高于N0水平;用脂磷含量表征土壤微生物含量,结果表明外加氮源对微生物含量有显著性影响,在N5、N10水平下,微生物含量在30~40 cm土层中出现峰值。

有机肥替代化肥氮素对麦田土壤碳氮迁移特征的影响

利用小麦田间试验,设置控释尿素(CRU)、有机肥(OF)替代30%,50%,70%控释尿素氮量处理,并以普通尿素(Urea)为对照,研究等氮条件下有机肥替代不同比例化肥氮素对土壤碳氮迁移特征及小麦产量的影响。结果表明:有机肥处理小麦总生物量较Urea显著增加13.83%~17.57%,籽粒产量增加1.6%~10.5%,随有机肥替代化肥氮素比例增加,籽粒增产效应降低,70%OF与Urea无显著差异,但显著低于CRU处理。CRU、30%OF和50%OF处理氮素农学效率较Urea显著提高90.2%~124.4%,70%OF与Urea相比差异不显著。有机肥比例增加,土壤总碳含量呈上升趋势,且高于CRU和Urea;全氮含量大致呈下降趋势,整个生育期先增加后降低,30%OF自灌浆期至成熟期含量高于其他施氮处理。随土层深度增加,硝态氮和铵态氮含量减少,有机肥比例增加,各层土壤硝态氮减少,铵态氮增加(尤以返青期最为显著);整个生育期土壤无机氮呈下降趋势,但与Urea相比,有机肥处理的硝态氮主要集中在0—40 cm土层,且0—100 cm土壤铵态氮含量高于Urea和CRU(苗期除外)。因此,用30%~50%有机肥替代化肥氮素,配合控释尿素施用,可显著增加土壤总碳和铵态氮含量,减少60—100 cm土壤硝态氮淋溶,提高小麦氮素利用率和籽粒产量。

Soil Carbon Balance in a Native Temperate Grassland in the Xilin River Basin of Inner Mongolia

Based on historical data and field investigation, some major fluxes and reserves of carbon were estimated, and a tentative analysis of the soil carbon balance was made in a native grassland community in the Xilin River basin of Inner Mongolia. Major results were reported as follows: 1) Annual average carbon input from above-ground biomass production was 79.8 g C(.)m(-2.)a(-1), and from root biomass to 30 cm. depth averaged 311.9 g C(.)m(-2.)a(-1). The summed mean annual carbon input of shoot and root materials in the study site was approximately 391.7 g C(.)m(-2.)a(-1). 2) The annual amount of above-ground biomass consumed by insects averaged 14.7 g C(.)m(-2.)a(-1), and the carbon output by leaching or light-chemical oxidation was 3.2 g C(.)m(-2.)a(-1) The annual evolution rate of CO2 from net soil respiration averaged 346.9 g C(.)m(-2.)a(-1), and the summed mean annual output was approximately 364.8 g C(.)m(-2.)a(-1). 3) A mature, steady-state system could be assumed for the community for which growth and decay were approximately in balance, with a net carbon accumulation of about 26.9 g C(.)m(-2.)a(-1). Based on the soil organic carbon density of the field, the turnover Irate of soil carbon in 0 - 30 cm depth was calculated to be 6.2%, with a turnover time of 16 years.

Effect of Long-Term Rice Straw Return on Soil Glomalin, Carbon and Nitrogen

A long-term experiment was conducted to investigate how long-term fertilization and rice straw incorporation into soil affect soil glomalin, C and N. The combined application of chemical fertilizer and straw resulted in a significant increase in both soil easily extractable glomalin (EEG) and total glomalin (TG) concentrations, as compared with application of only chemical fertilizer or no fertilizer application. The EEG and TG concentrations of the NPKS (nitrogen, phosphorus, and potassium fertilizer application + rice straw return) plot were 4.68% and 5.67% higher than those of the CK (unfertilized control) plot, and 9.87% and 6.23% higher than those of the NPK (nitrogen, phosphorus, and potassium fertilizer applied annually) plot, respectively. Application of only chemical fertilizer did not cause a statistically significant change of soil glomalin compared with no fertilizer application. The changes of soil organic C (SOC) and total N (TN) contents demonstrated a similar trend to soil glomalin in these plots. The SOC and TN contents of NPKS plot were 15.01% and 9.18% higher than those of the CK plot, and 8.85% and 14.76% higher than those of the NPK plot, respectively. Rice straw return also enhanced the contents of microbial biomass C (MBC) and microbial biomass N (MBN) in the NPKS plot by 7.76% for MBC and 31.42% for MBN compared with the CK plot, and 12.66% for MBC and 15.07% for MBN compared with the NPK plots, respectively. Application of only chemical fertilizer, however, increased MBN concentration, but decreased MBC concentration in soil.

Relationships among Glomalin Related Soil Protein,Soc and Soil Texture under Different Land Use Types

[Objective] The aim was to reveal the effects of different land use types on soil composition. [Method] GPRS,soil organic carbon content and soil texture in 3 depths (0-10,10-20,20-50 cm) of 5 main kind of selected land use type were examined in Hainan. [Result] The results showed that GRSP and SOC content of four artificial land use types decreased compared with the natural secondary forest land,the GRSP content of all samples ranged from 0.53-4.80 mg/g,accounting for 7.9%-23.4% of the SOC,which means that GRSP was one important component of SOC pool in soil. The ratio of GRSP to SOC was significantly different among land use types but the depths. GRSP and SOC exhibited obvious vertical distribution pattern. GRSP was significantly positively related to SOC and sand content but negatively related to silt and clay content. [Conclusion] The sand content determined the GRSP content significantly and loam was better matrix for GRSP accumulation than clay.

Scale Effect of Climate and Soil Texture on Soil Organic Carbon in the Uplands of Northeast China

Understanding how spatial scale influences commonly-observed effects of climate and soil texture on soil organic carbon （SOC） storage is important for accurately estimating the SOC pool at different scales. The relationships among climate factors, soil texture and SOC density at the regional, provincial, city, and county scales were evaluated at both the soil surface （0-20 cm） and throughout the soil profile （0-100 cm） in the Northeast China uplands. We examined 1 022 profiles obtained from the Second National Soil Survey of China. The results indicated that the relationships between climate factors and SOC density generally weakened with decreasing spatial scale. The provincial scale was optimal to assess the relationship between climate factors and SOC density because regional differences among provinces were covered up at the regional scale. However, the relationship between soil texture and SOC density had no obvious trend with increasing scale and changed with temperature. There were great differences in the impacts of climate factors and soil texture on SOC density at different scales. Climate factors had a larger effect on SOC density than soil texture at the regional scale. Similar trends were seen in Heilongjiang and eastern Inner Mongolia at the provincial scale. But, soil texture had a greater effect on SOC density compared with climate factors in Jilin and Liaoning. At the city and county scales, the influence of soil texture on SOC density was more important than climate factors.

Stable Carbon and Oxygen Isotopes of Pedogenic Carbonates in Ustic Vertisols: Implications for Paleoenvironmental Change

Pedogenic carbonates, found extensively in arid and semiarid regions, are important in revealing regional climatic and environmental changes as well as the carbon cycle. In addition, stable carbon and oxygen isotopic compositions of pedogenic carbonates have been used to rebuild paleoecology (biomass and vegetation) and to estimate paleotemperature and paleoprecipitation during past geological time. By utilizing the stable carbon and oxygen isotopic compositions (δ13C and (δ18O) of secondary nodules in Ustic Vertisols, this study looked into the climatic and environmental changes in the dry valleys of the Yuanmou Basin, Yunnan Province, in southwestern China. The results showed that during the early Holocene, a warm-humid or hot-humid climate existed in the Yuanmou Basin, but since then fluctuations in climate have occurred, with a dry climate prevailing. A highly significant correlation (r = 0.92, n = 9) between δ13C and δ18O values of carbonates illustrated that there had been a continual shifting between cold-humid and warm-dry climates in southwestern China including the Yuanmou Basin since the early Holocene.

Light Fraction Carbon and Water-Stable Aggregates in Black Soils

The distribution of light fraction carbon （LF-C） in the various size classes of aggregates and its relationship to water- stable aggregates as well as the influence of cultivation on the organic components in virgin and cultivated black soils were studied by wet sieving and density separation methods. The total organic carbon （TOC） and LF-C were significantly higher （P≤ 0.05） in the virgin soils than in the cultivated soils. The LF-C in aggregates of different size classes varied from 0.9 to 2.5 g kg^-1 in the cultivated soils and from 2.5 to 7.1 g kg^-1 in the virgin soils, whereas the ratio of LF-C to TOC varied from 1.9% to 7.3% and from 5.0% to 12.2%, respectively. After being incubated under constant temperature and controlled humidity for three months, the contribution of LF-C to TOC sharply decreased to an amount （1.7%4.5%） close to the level in soils that had been cultivated for 20 to 25 years （1.3%-8.8%）. As a result, the larger water-stable macro-aggregates （especially 〉 1 mm） decreased sharply, indicating that the LF-C pool in virgin soils declined quickly after cultivation, which reduced the water stability of soil aggregates.

Effects of Soil Texture and CaCO_3 on Turnover of Organic Material in Chao Soils

Decomposition experiments of 14C-labelled sickle alfalfa in chao soils of different texture and these soils after removal of CaCO3 were carried out under field and laboratory conditions respectively. The amount of residual 14C in, or 14CO2 evolved from, the soils at intervals after the beginning of decomposition were measured and the distribution of native and labelled C between particle size fractions isolated from these soils was edtermined. Results showed that contents of both labelled (14C) and non-labelled (12C) carbon decreased with increasing particle size. The enrichment factor for 14C was higher than that for 12C in the clay fraction, the reverse being true for the silt enrichment factors. The effect of soil texture on the decomposition of plant material could not be observed in chao soils when the clay content was lower than 270g kg-1, while it became obvious once CaCO3 was removed from these soils. The decomposition rate of plant material in the soil from which the native CaCO3 was removed was correlated significantly to both the clay content of the soil and the application rate of CaCO3. A preliminary correction equation describing the effect of clay and CaCO3 on the decomposition of organic material in chao soil was derived from the results obtained.

Characteristics of heavy metals in soils under different land use in a typical karst area, Southwest China

This study investigated the distribution of six pollutant elements(Cr, Cu, Pb, Zn, Cd, and As), and their relationship to soil organic carbon(SOC) in five soil profiles in the Puding area. Results show SOC content decreased exponentially to the depth of soil profiles; the vertical distribution patterns of SOC in soil profiles were partially controlled by land use. The concentrations of these pollutant elements in most soils were lower than background values, indicating that the local soil was less likely to be contaminated by foreign inputs. Geo-accumulation index values of these elements in most soil samples were less than 1, suggesting that the soil of this area may not be polluted. The concentrations of Cr, Cu, As, and Zn in soils from all land use types were significantly negatively correlated with SOC contents. Geochemical approaches confirmed that the soil of this area was less influenced by pollutant elements.

Spatial Variability of Soil Carbon to Nitrogen Ratio and Its Driving Factors in Ili River Valley,Xinjiang,Northwest China

Soil carbon to nitrogen(C/N) ratio is one of the most important variables reflecting soil quality and ecological function,and an indicator for assessing carbon and nitrogen nutrition balance of soils.Its variation reflects the carbon and nitrogen cycling of soils.In order to explore the spatial variability of soil C/N ratio and its controlling factors of the Ili River valley in Xinjiang Uygur Autonomous Region,Northwest China,the traditional statistical methods,including correlation analysis,geostatistic alanalys and multiple regression analysis were used.The statistical results showed that the soil C/N ratio varied from 7.00 to 23.11,with a mean value of 10.92,and the coefficient of variation was 31.3%.Correlation analysis showed that longitude,altitude,precipitation,soil water,organic carbon,and total nitrogen were positively correlated with the soil C/N ratio(P < 0.01),whereas negative correlations were found between the soil C/N ratio and latitude,temperature,soil bulk density and soil p H.Ordinary Cokriging interpolation showed that r and ME were 0.73 and 0.57,respectively,indicating that the prediction accuracy was high.The spatial autocorrelation of the soil C/N ratio was 6.4 km,and the nugget effect of the soil C/N ratio was 10% with a patchy distribution,in which the area with high value(12.00–20.41) accounted for 22.6% of the total area.Land uses changed the soil C/N ratio with the order of cultivated land > grass land > forest land > garden.Multiple regression analysis showed that geographical and climatic factors,and soil physical and chemical properties could independently explain 26.8%and 55.4% of the spatial features of soil C/N ratio,while human activities could independently explain 5.4% of the spatial features only.The spatial distribution of soil C/N ratio in the study has important reference value for managing soil carbon and nitrogen,and for improving ecological function to similar regions.

宁南山区典型植物根际与非根际土壤碳、氮形态

以宁南山区典型植物冰草、冷蒿、长芒草、百里香和铁杆蒿为对象,研究不同植物根际土壤和非根际土壤碳、氮形态的变化.结果表明:5种植物对根际土壤和非根际土壤碳、氮含量的影响不同.其中,铁杆蒿的根际土壤碳含量最高,总有机碳、轻组有机碳和重组有机碳含量分别为22.94、1.95和20.88g·kg-1,长芒草的根际土壤氮含量最高,总氮、可矿化氮和速效氮含量分别为2.05g·kg-1、23.73mg·kg-1和11.99mg·kg-1.冷蒿的根际土壤中活性有机碳/总有机碳、可矿化氮/总氮最高,有利于土壤中碳素和氮素向活性态转变.轻组有机碳、可矿化氮可作为植物生境改变的敏感指标.5种植物根际土壤各形态碳、氮含量总体上高于非根际土壤.

Transformation of Straw 14 C in Ultisol and Vertisol *1

Laboratory incubation was conducted to investigate transformation of straw 14 C in Ultisol and Vertisol under aerobic condition for 112 d at 30 oC. Dried and ground 14 C labeled rice and maize straws were mixed with the soils at the rate of 2.5 g kg -1 . Decomposition of the straw C and native soil C both revealed two stages, being faster during the initial days, and slower thereafter. About 37.33%～48.80% of the straw C and 4.22%～6.83% of the native soil C decomposed by the end of the incubation. The kinds of the straws only slightly influenced the rates of their decomposition in soils, however, some retardation was found in Ultisol at the initial decomposition stage due to its lower pH. Positive priming effects were observed in the soils applied with straw, and the rate of priming effect ranged from 7.23% to 13.80%. Net losses of native soil C were found under such incubation conditions, except Ultisol with rice straw. Soil biomass C and 14 C decreased gradually with incubation time, and seemed to be consistent with the decomposition patterns of straw C and native soil C. The ratio of biomass 12 C to biomass 14 C ranged from 1.35 to 3.37. Soil biomass C occupied 1.17%～2.32% of the total soil organic C, and the proportion of biomass 14 C to the residual 14 C varied from 7.3% to 14.3%.

The hydrologic record of karst systems: linking soil moisture to the carbon isotope signatures of soils above the Blue Spring cave system

Speleothem carbonates are precipitated continuously from inorganic carbon dissolved in seepage water infiltrating from the land surface,that reflects a mixture of atmospheric CO_2,respired soil carbon,and epikarst sources,each with distinct δ^(13)C values.To aid in deconvolving these signatures,soil samples were collected above the Blue Spring cave system in Sparta,Tennessee,USA and subjected to a series of incubation experiments,in order to constrain the correlation between CO_2respiration rates and soil moisture.This relationship is used to parameterize a simple mixing model which predicts the relationship between δ^(13)C and soil moisture in fluids infiltrating into the underlying cave system.

Soil organic carbon dynamics study bias deduced from isotopic fractionation in corn plant

Carbon stable isotope techniques were extensively employed to trace the dynamics of soil organic carbon(SOC)across a land-use change involving a shift to vegetation with different photosynthetic pathways.Based on the isotopic mass balance equation,relative contributions of new versus old SOC,and SOC turnover rate in corn fields were evaluated world-wide.However,most previous research had not analyzed corn debris left in the field,instead using an average corn plant δ^(13)C value or a measured value to calculate the proportion of corn-derived SOC,either of which could bias results.This paper carried out a detailed analysis of isotopic fractionation in corn plants and deduced the maximum possible bias of SOC dynamics study.The results show approximately 3‰ isotopic fractionation from top to bottom of the corn leaf.The ^(13)C enrichment sequence in corn plant was tassel﹥stalk or cob﹥root﹥leaves.Individual parts accounting for the total dry mass of corn returned distinct values.Consequently,the average δ^(13)C value of corn does not represent the actual isotopic composition of corn debris.Furthermore,we deduced that the greater the fractionation in corn plant,the greater the possible bias.To alleviate bias of SOC dynamics study,we suggest two measures:analyze isotopic compositions and proportions of each part of the corn and determine which parts of the corn plant are left in the field and incorporated into SOC.

Application of NaHCO_3/ DTPA Extractant-ICP Spectrometry Technique in Soil Test for Availability of Nutrients and Heavy Metals

Soil test for availability of nutrients and heavy metals is extensively served as a means for the evaluations of soil fertility, and environmental effects and phytotoxicity of pollutants in soils, and for the fertilizer recommendation in agricultural and environmental sciences. Therefore, great attention has been paid to the measurement of elemental availability in soil test.

浙江省两年五熟制吨粮田的碳素平衡研究

对三种主要水稻土测定结果表明,紫云英与黑麦草混播,其腐殖化系数比各自单播提高25.7%和11.8%.脱潜水稻土、淹育水稻土和潴育水稻土等三种吨粮田的土壤碳年矿化量分别为1806.0、1529.6和1548.0kgha^(-1),平均为1627.5kgha^(-1).单施化肥时,只是归还根茬碳728.9kgha^(-1),尚相差898.7kgha^(-1),而化肥加有机肥处理者,则可维持土壤碳素平衡.施化肥年平均亩产量虽可超一吨,但化肥加施有机肥可比单施化肥增产9.1%.

Different Carbon Fractions in Soils and Their Relationship with Trace Elements Content

Luvisols, Stagnogleys and Cambisols, although less fertile, are used intensively for mixed farming, grazing and as forestland. Therefore we aimed our study at determination of total organic carbon （TOC） content, humic substances （HS） content, humic acids （HA） content, fulvic acids （FA）, hot water extractable carbon （Chw） content and content total and labile trace elements content. Humic substances quality was assessed by HA/FA ratio and by coloured indexes measured in ultraviolet and visible UV-VIS spectral range. The total and labile contents of Zn, Cd, Cu, Co, Pb, Mo and Se were determined by flame or electro-thermal atomic absorption spectrometry after extraction of the soil samples in the aqua regia （total content） and in the solution of 0.01 M CaCI2. Total and labile trace elements content was correlated with determined carbon fractions and soil reaction. Results showed that studied soils content low amount of TOC and had low quality of humic substances. HA/FA ratio was less than 1 and colour indexes were higher than 4. All determined carbon fractions correlated with labile form of Zn and Cd. Correlation between soil reaction and total zinc content was found. Significant effect of humic substances content on to water-soluble forms of heavy metals was detected.

长期定位试验下施肥对潮棕壤活性碳库及碳库管理指数的影响

分析了长期不同施肥制度对潮棕壤活性碳库及碳库管理指数的影响。结果表明:长期施肥不同施肥制度对土壤活性碳库、碳素有效率及碳库管理指数有很大影响,长期施用有机循环肥处理,尤其是循环肥配合均衡化肥(NPK+O)施用处理的土壤有机碳、易氧化碳(ROC)、溶解性有机碳(DOC)、微生物量碳含量(MBC)、土壤碳素有效率及土壤碳库管理指数明显高于对照处理的土壤。潮棕壤活性碳库中不同形态的碳素含量大小顺序为:CROC>CMBC>CDOC,同一施肥处理水平下,不同形态碳素有效率以ACC>ABC>AAC。相关分析表明:土壤碳素有效率和碳库管理指数与土壤的化学性质尤其是土壤养分因子呈显著或极显著相关,反应了农业生产措施对土壤碳库的影响,运用土壤碳库管理指数可以很好地反映出土壤碳库的动态变化。