Comparative study on the activity and kinectic properties of acid phosphatase （ACPase） of three soils amended with Hg and Cu at constant temperature and humidity was carried out. The results indicated that the inhib...Comparative study on the activity and kinectic properties of acid phosphatase （ACPase） of three soils amended with Hg and Cu at constant temperature and humidity was carried out. The results indicated that the inhibition on ACPase of the three sample soils by Hg and Cu varied with the content of soil organic matter and pH, where, Soil 1 was the most seriously contaminated due to its lowest content of organic matter and the lowest pH among three samples, Soil 2 took the second place, and Soil 3 was the least contaminated. Except Soil 3, the activity of soil ACPase tended to increase along with the contact time under the same type and the same concentration of heavy metal. In particular the Vmax values of ACPase in all three samples decreased with increasing Hg and Cu concentration, whereas the Km values were affected weakly. According to the change of Vmax and Km values, Cu and Hg had the same inhibition effect on soil ACPase. Both of them may he a type of compound of non-competitive and anti-competitive inhibition. Statistic analyses indicated that activities of soil ACPase and Vmax values could serve as bioindicator to partially denote the heavy metal Hg and Cu contamination degree.展开更多
Organophosphorus pesticides （OPs） are one of the most regular pollutants and frequently detected in the contaminated sites, so developing an efficient method for the treatment of OPs is highly required. The aim of t...Organophosphorus pesticides （OPs） are one of the most regular pollutants and frequently detected in the contaminated sites, so developing an efficient method for the treatment of OPs is highly required. The aim of the present study was to compare the effectiveness of persulfate （PS） activation and Fenton reaction in remediating the soil polluted with OPs. The polluted soil used in this study was sampled from an abandoned insecticide factory in Nantong, Jiangsu Province of China, mainly containing chloropyrifos （CP） and 4-bromo-2-chlorophenol （BCP, the raw material of profenofos） with total concentration of about 30 000 mg kg- 1. The results showed that both BCP and CP were efficiently degraded by base activation of PS, and increasing the ratio of NaOH/PS enhanced CP degradation, but slightly decreased BCP degradation. The greatest degradation rates for CP and BCP were 92% and 97%, respectively, with 7.0 tool L-1 NaOH and 0.21 tool L-1 PS and a soil-to-liquid ratio of 1：1. Furthermore, ferrous iron activation of PS also degraded BCP efficiently, but only 60% of CP was degraded under the same reaction conditions. These results indicated that base activation of PS was more feasible than Fe2＋ activation and Fenton reaction in remediating the soil polluted with OPs. The high degradation rate for CP may be linked to the initial hydrolyzation of CP by base to 3,5,6-trichloro-2-pyridinol, which can be further rapidly degraded by free radicals generated from base activation of PS.展开更多
基金Project (No. 2002CB410800) supported by the National NaturalScience Foundation of China
文摘Comparative study on the activity and kinectic properties of acid phosphatase （ACPase） of three soils amended with Hg and Cu at constant temperature and humidity was carried out. The results indicated that the inhibition on ACPase of the three sample soils by Hg and Cu varied with the content of soil organic matter and pH, where, Soil 1 was the most seriously contaminated due to its lowest content of organic matter and the lowest pH among three samples, Soil 2 took the second place, and Soil 3 was the least contaminated. Except Soil 3, the activity of soil ACPase tended to increase along with the contact time under the same type and the same concentration of heavy metal. In particular the Vmax values of ACPase in all three samples decreased with increasing Hg and Cu concentration, whereas the Km values were affected weakly. According to the change of Vmax and Km values, Cu and Hg had the same inhibition effect on soil ACPase. Both of them may he a type of compound of non-competitive and anti-competitive inhibition. Statistic analyses indicated that activities of soil ACPase and Vmax values could serve as bioindicator to partially denote the heavy metal Hg and Cu contamination degree.
基金supported by the National Key Basic Research Program of China (No. 2013CB934303)the Environmental Protection Program of Jiangsu Province of China (No. 2015011)the Youth Innovation Promotion Association of Chinese Academy of Sciences (No. 2014270)
文摘Organophosphorus pesticides （OPs） are one of the most regular pollutants and frequently detected in the contaminated sites, so developing an efficient method for the treatment of OPs is highly required. The aim of the present study was to compare the effectiveness of persulfate （PS） activation and Fenton reaction in remediating the soil polluted with OPs. The polluted soil used in this study was sampled from an abandoned insecticide factory in Nantong, Jiangsu Province of China, mainly containing chloropyrifos （CP） and 4-bromo-2-chlorophenol （BCP, the raw material of profenofos） with total concentration of about 30 000 mg kg- 1. The results showed that both BCP and CP were efficiently degraded by base activation of PS, and increasing the ratio of NaOH/PS enhanced CP degradation, but slightly decreased BCP degradation. The greatest degradation rates for CP and BCP were 92% and 97%, respectively, with 7.0 tool L-1 NaOH and 0.21 tool L-1 PS and a soil-to-liquid ratio of 1：1. Furthermore, ferrous iron activation of PS also degraded BCP efficiently, but only 60% of CP was degraded under the same reaction conditions. These results indicated that base activation of PS was more feasible than Fe2＋ activation and Fenton reaction in remediating the soil polluted with OPs. The high degradation rate for CP may be linked to the initial hydrolyzation of CP by base to 3,5,6-trichloro-2-pyridinol, which can be further rapidly degraded by free radicals generated from base activation of PS.
