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摩西管柄囊霉(Funneliformis mosseae)对连作大豆根际土壤细菌菌群的影响 被引量:4
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作者 崔晓莹 白莉 +2 位作者 郭娜 殷继忠 蔡柏岩 《大豆科学》 CAS CSCD 北大核心 2020年第2期277-287,共11页
为探究丛枝菌根真菌(Arbuscula mycorrhizal fungi,AMF)-摩西管柄囊霉(Funneliformis mosseae)对不同连作年限大豆土壤细菌菌群的影响,分析了接种F.mosseae后连作0,1,3和5年的根系菌根侵染率、细菌菌群结构和多样性以及KEGG功能预测,从... 为探究丛枝菌根真菌(Arbuscula mycorrhizal fungi,AMF)-摩西管柄囊霉(Funneliformis mosseae)对不同连作年限大豆土壤细菌菌群的影响,分析了接种F.mosseae后连作0,1,3和5年的根系菌根侵染率、细菌菌群结构和多样性以及KEGG功能预测,从而为AMF缓解大豆连作障碍提供理论依据。结果表明:大豆土壤细菌菌群多样性和丰度随着连作年限升高而减少,鞘氨醇单胞菌属(Sphingomonas)、芽单胞菌属(Gemmatimonas)、芽孢杆菌属(Bacillus)和硝化杆菌属(Nitrobacter)等有益菌丰度也随着连作年限升高而减少。而接种F.mosseae不仅能改善根际土壤的细菌多样性,提高Sphingomonas、Gemmatimonas、Nitrospira和Lysobacter等有益菌的丰度,还改善了根际细菌群落氨基酸代谢、强化信号转导、膜转运和碳水化合物代谢等功能,显著缓解了大豆连作障碍。 展开更多
关键词 连作大豆 摩西管柄囊霉 土壤细菌菌群 KEGG功能预测
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对二氯苯对湿地土壤细菌群落多样性的影响 被引量:1
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作者 徐文品 丁成 +2 位作者 杨春生 朱光灿 杨唐仪 《湖北农业科学》 北大核心 2012年第18期4134-4137,共4页
利用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)考查了对二氯苯胁迫对湿地土壤细菌群落多样性的影响。通过不同培养时间(4、18和45d)和不同培养浓度(120、240、360和600mg/kg)对二氯苯污染下湿地土壤细菌群落基因组的变性梯度凝胶电泳(... 利用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)考查了对二氯苯胁迫对湿地土壤细菌群落多样性的影响。通过不同培养时间(4、18和45d)和不同培养浓度(120、240、360和600mg/kg)对二氯苯污染下湿地土壤细菌群落基因组的变性梯度凝胶电泳(DGGE)多样性分析。结果表明,各处理下的土壤细菌群落多样性在不同处理时间有明显差异。培养初期对二氯苯对土壤细菌群落抑制作用非常显著,土壤细菌中某些群落的生长受到抑制;不同处理在处理后期差异很小。在土壤培养初期,含对二氯苯240mg/kg的培养基上出现一条新增条带,为对照和低浓度对二氯苯土壤所没有,说明土壤中存在对二氯苯生长优势菌。 展开更多
关键词 变性梯度凝胶电泳(DGGE) 对二氯苯 湿地土壤细菌菌群 多样性
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A Review of Methods for Studying Microbial Diversity in Soils 被引量:74
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作者 LIU Bing-Ru JIA Guo-Mei +1 位作者 CHEN Jian WANG Gang 《Pedosphere》 SCIE CAS CSCD 2006年第1期18-24,共7页
Soil microorganisms play a central role in decomposing organic matter, in determining the release of mineral nutrients, and in nutrient cycling. Recently, extensive studies have focused on soil microbial diversity. Ho... Soil microorganisms play a central role in decomposing organic matter, in determining the release of mineral nutrients, and in nutrient cycling. Recently, extensive studies have focused on soil microbial diversity. However, understanding the diversity of this complex microbial community in the soil environment is a challenging task. Thus, it is important to master and comprehend appropriate methods for studying soil microbial diversity. Concepts of soil microbial diversity and major methods of study are briefly introduced in this paper. Then, the application of biochemical-based and molecular-based techniques in this area, and their advantages and disadvantages are evaluated. Based on recent related research, perspectives for studying microbial diversity in soils are presented. 展开更多
关键词 BACTERIA DIVERSITY FUNGI MOLECULAR soil
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Effects of Slope Aspects on Soil Bacterial and Arbuscular Fungal Communities in a Boreal Forest in China 被引量:17
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作者 CHU Haiyan XIANG Xingjia +4 位作者 YANG Jian Jonathan M. ADAMS ZHANG Kaoping LI Yuntao SHI Yu 《Pedosphere》 SCIE CAS CSCD 2016年第2期226-234,共9页
The effects of slope aspects on soil biogeochemical properties and plant communities in forested environments have been studied extensively; however, slope aspect influence on soil microbial communities remains largel... The effects of slope aspects on soil biogeochemical properties and plant communities in forested environments have been studied extensively; however, slope aspect influence on soil microbial communities remains largely unexamined, despite the central role of soil biota in ecosystem functioning. In this study, the communities of both soil bacteria and arbuscular mycorrhizal fungi (AMF) were investigated using tagged pyrosequencing for three types of slope aspects (south-facing aspect, north-facing aspect and flat area) in a boreal forest of the Greater Khingan Mountains, China. The bacterial and AMF community composition differed with slope aspects. Bacterial diversity was the lowest on the north-facing aspect, and AMF diversity was the lowest on the flat area. Aspects also had a significant impact on soil pH and available phosphorus (P) and shrubby biomass. Soil pH and understory shrub biomass were significantly correlated with bacterial communities, and soil available P and shrub biomass showed significant correlations with AMF communities. Our results suggested that slope aspects affected bacterial and AMF communities, mediated by aspect-induced changes in plant community and soil chemical properties (e.g., pH and available P), which improved the knowledge on the effects of forest slope aspects on aboveground and belowground communities. 展开更多
关键词 available P plant communities PYROSEQUENCING shrubby biomass soil chemical properties soil microbial communities soil pH
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