As one of the main methods of microbial community functional diversity measurement, biolog method was favored by many researchers for its simple oper- ation, high sensitivity, strong resolution and rich data. But the ...As one of the main methods of microbial community functional diversity measurement, biolog method was favored by many researchers for its simple oper- ation, high sensitivity, strong resolution and rich data. But the preprocessing meth- ods reported in the literatures were not the same. In order to screen the best pre- processing method, this paper took three typical treatments to explore the effect of different preprocessing methods on soil microbial community functional diversity. The results showed that, method B＇s overall trend of AWCD values was better than A and C＇s. Method B＇s microbial utilization of six carbon sources was higher, and the result was relatively stable. The Simpson index, Shannon richness index and Car- bon source utilization richness index of the two treatments were B〉C〉A, while the Mclntosh index and Shannon evenness were not very stable, but the difference of variance analysis was not significant, and the method B was always with a smallest variance. Method B＇s principal component analysis was better than A and C＇s. In a word, the method using 250 r/min shaking for 30 minutes and cultivating at 28 ℃ was the best one, because it was simple, convenient, and with good repeatability.展开更多
A data processing method was proposed for eliminating the end restraint in triaxial tests of soil. A digital image processing method was used to calculate the local deformations and local stresses for any region on th...A data processing method was proposed for eliminating the end restraint in triaxial tests of soil. A digital image processing method was used to calculate the local deformations and local stresses for any region on the surface of triaxial soil specimens. The principle and implementation of this digital image processing method were introduced as well as the calculation method for local mechanical properties of soil specimens. Comparisons were made between the test results calculated by the data from both the entire specimen and local regions, and it was found that the deformations were more uniform in the middle region compared with the entire specimen. In order to quantify the nonuniform characteristic of deformation, the non-uniformity coefficients of strain were defined and calculated. Traditional and end-lubricated triaxial tests were conducted under the same condition to investigate the effects of using local region data for deformation calculation on eliminating the end restraint of specimens. After the statistical analysis of all test results, it was concluded that for the tested soil specimen with the size of 39.1 mm × 80 ram, the utilization of the middle 35 mm region of traditional specimens in data processing had a better effect on eliminating end restraint compared with end lubrication. Furthermore, the local data analysis in this paper was validated through the comparisons with the test results from other researchers.展开更多
基金Supported by National and International Scientific and Technological Cooperation Project"The application of Microbial Agents on Mining Reclamation and Ecological Recovery"(2011DFR31230)Key Project of Shanxi academy of Agricultural Science"The Research and Application of Bio-organic Fertilizer on Mining Reclamation and Soil Remediation"(2013zd12)Major Science and Technology Programs of Shanxi Province"Key Technology Research and Demonstration of mining waste land ecosystem Restoration and Reconstruction"(20121101009)~~
文摘As one of the main methods of microbial community functional diversity measurement, biolog method was favored by many researchers for its simple oper- ation, high sensitivity, strong resolution and rich data. But the preprocessing meth- ods reported in the literatures were not the same. In order to screen the best pre- processing method, this paper took three typical treatments to explore the effect of different preprocessing methods on soil microbial community functional diversity. The results showed that, method B＇s overall trend of AWCD values was better than A and C＇s. Method B＇s microbial utilization of six carbon sources was higher, and the result was relatively stable. The Simpson index, Shannon richness index and Car- bon source utilization richness index of the two treatments were B〉C〉A, while the Mclntosh index and Shannon evenness were not very stable, but the difference of variance analysis was not significant, and the method B was always with a smallest variance. Method B＇s principal component analysis was better than A and C＇s. In a word, the method using 250 r/min shaking for 30 minutes and cultivating at 28 ℃ was the best one, because it was simple, convenient, and with good repeatability.
基金Supported by Major State Basic Research Development Program of China("973" Program,No.2010CB731502)
文摘A data processing method was proposed for eliminating the end restraint in triaxial tests of soil. A digital image processing method was used to calculate the local deformations and local stresses for any region on the surface of triaxial soil specimens. The principle and implementation of this digital image processing method were introduced as well as the calculation method for local mechanical properties of soil specimens. Comparisons were made between the test results calculated by the data from both the entire specimen and local regions, and it was found that the deformations were more uniform in the middle region compared with the entire specimen. In order to quantify the nonuniform characteristic of deformation, the non-uniformity coefficients of strain were defined and calculated. Traditional and end-lubricated triaxial tests were conducted under the same condition to investigate the effects of using local region data for deformation calculation on eliminating the end restraint of specimens. After the statistical analysis of all test results, it was concluded that for the tested soil specimen with the size of 39.1 mm × 80 ram, the utilization of the middle 35 mm region of traditional specimens in data processing had a better effect on eliminating end restraint compared with end lubrication. Furthermore, the local data analysis in this paper was validated through the comparisons with the test results from other researchers.
