挤出机停留时间分布(Residence time distribution,RTD)反映物料在挤出过程中所受的热历史,可以表征其轴向混合能力。基于荧光原理,借助转矩流变仪的单螺杆挤出平台搭建一套挤出机RTD在线检测装置。利用荧光分光光度计确定不同示踪剂的...挤出机停留时间分布(Residence time distribution,RTD)反映物料在挤出过程中所受的热历史,可以表征其轴向混合能力。基于荧光原理,借助转矩流变仪的单螺杆挤出平台搭建一套挤出机RTD在线检测装置。利用荧光分光光度计确定不同示踪剂的最佳测量波长,进一步探明示踪剂浓度和光信号的线性关系;在优选蒽(EN)为示踪剂的基础上,通过重复实验考察此荧光在线检测装置的可靠性,并确定示踪剂用量同时也考察螺杆转速对聚苯乙烯(Polystyrene,PS)在单螺杆挤出过程RTD的影响。结果表明:该荧光在线检测装置可以成功检测到PS在单螺杆挤出机中的停留时间分布且稳定性良好;螺杆转速越快、最小停留时间越小、停留时间分布变窄,表明挤出机的轴向混合能力减小。展开更多
Human erythropoietin （hEPO）, an endogenous glycoprotein, plays a fundamental role in erythropoiesis controlling the formation of red blood cells. Production of recombinant human erythropoietin （rhEPO） has made it ...Human erythropoietin （hEPO）, an endogenous glycoprotein, plays a fundamental role in erythropoiesis controlling the formation of red blood cells. Production of recombinant human erythropoietin （rhEPO） has made it possible for its abuse in competitive sports. In this work, pre-capillary and on-capillary derivatization by 5-furoylquinoline-3-carboxaldehyde （FQ） and fluorescein isothiocyanate （FITC） for the detection of rhEPO by capillary electrophoresis with laser-induced fluorescence detection （CE-LIF） were compared. FQ pre-capillary labeling improves sensitivity but degrades the glycoforms separation due to the inhomogeneity of the reaction products from multiple labeling. Compared with FITC pre-capillary derivatization with the excess fluorescent background, the on-capillary FQ derivatization method can provide shorter analysis time, lower background, and better selectivity. It is demonstrated that, through optimizing reaction conditions of FQ on-capillary derivatization, both high sensitivity and satisfactory resolution for the analysis of the be used for the glycoforms profiling and quality control of rhEPO doping control analysis. glycoforms of rhEPO could be obtained. This method can It may be used as a candidate method for fast screening in展开更多
文摘挤出机停留时间分布(Residence time distribution,RTD)反映物料在挤出过程中所受的热历史,可以表征其轴向混合能力。基于荧光原理,借助转矩流变仪的单螺杆挤出平台搭建一套挤出机RTD在线检测装置。利用荧光分光光度计确定不同示踪剂的最佳测量波长,进一步探明示踪剂浓度和光信号的线性关系;在优选蒽(EN)为示踪剂的基础上,通过重复实验考察此荧光在线检测装置的可靠性,并确定示踪剂用量同时也考察螺杆转速对聚苯乙烯(Polystyrene,PS)在单螺杆挤出过程RTD的影响。结果表明:该荧光在线检测装置可以成功检测到PS在单螺杆挤出机中的停留时间分布且稳定性良好;螺杆转速越快、最小停留时间越小、停留时间分布变窄,表明挤出机的轴向混合能力减小。
基金National Natural Science Foundation of China(Grant No.20635001 and 21175005)
文摘Human erythropoietin （hEPO）, an endogenous glycoprotein, plays a fundamental role in erythropoiesis controlling the formation of red blood cells. Production of recombinant human erythropoietin （rhEPO） has made it possible for its abuse in competitive sports. In this work, pre-capillary and on-capillary derivatization by 5-furoylquinoline-3-carboxaldehyde （FQ） and fluorescein isothiocyanate （FITC） for the detection of rhEPO by capillary electrophoresis with laser-induced fluorescence detection （CE-LIF） were compared. FQ pre-capillary labeling improves sensitivity but degrades the glycoforms separation due to the inhomogeneity of the reaction products from multiple labeling. Compared with FITC pre-capillary derivatization with the excess fluorescent background, the on-capillary FQ derivatization method can provide shorter analysis time, lower background, and better selectivity. It is demonstrated that, through optimizing reaction conditions of FQ on-capillary derivatization, both high sensitivity and satisfactory resolution for the analysis of the be used for the glycoforms profiling and quality control of rhEPO doping control analysis. glycoforms of rhEPO could be obtained. This method can It may be used as a candidate method for fast screening in
