The present study describes the frequency of Foot and Mouth Disease （FMD） virus serotypes （O, A and Asia-l） in major regions （all provinces） of Pakistan using Indirect Sandwich ELISA. Also, spatial distribution ...The present study describes the frequency of Foot and Mouth Disease （FMD） virus serotypes （O, A and Asia-l） in major regions （all provinces） of Pakistan using Indirect Sandwich ELISA. Also, spatial distribution of various FMD serotypes and their comparison is discussed. A total of 590 samples （Epithelial tissue） have been analyzed during a period of five years （2005-2009）. Out of 590 samples, 180 were found positive, giving an overall confirmation of FMDV about 33.2 %. Of the prevalent serotypes, FMDV ＇O＇ serotype caused most outbreaks （20.7 %）, followed by serotype A （6.6 %） and serotype Asia-1 （4.6 %） while there was no positive case of type ＇C＇. The study clearly showed that the disease was more frequent in the agro-climatic zones than in hilly areas. Based on the data of 590 samples （〉50 outbreaks）, the overall prevalence of FMDV in cattle and buffaloes in Pakistan was 33.2 %, while in cattle alone, it was 37.1%, higher than in buffalo （28.7 %）. There were eight cases of mixed serotypes infection, indicating the presence of endemic state of disease. Another significant feature was the change over time. In phase-I （2005-2007）, there was an overall prevalence of 29.4 %, while the occurrence of the serotype O, A and Asia-1 was 20.4 %, 2.9 % and 4.7 %, respectively. During phase-II （2008-2009）, the overall prevalence was 59.21%, while those of serotype O, A and Asia-1 were 22.4 %, 31.6 % and 4.0 %, respectively. This clearly indicated a shift from serotype O to A, which may help to explain the occurrence of more severe outbreaks, despite vaccination.展开更多
The basic principle of interval arithmetic and the basic algorithm of the interval Newton methods are introduced.The prototype algorithm can not find any zero in an interval that has zero sometimes,that is,it is insta...The basic principle of interval arithmetic and the basic algorithm of the interval Newton methods are introduced.The prototype algorithm can not find any zero in an interval that has zero sometimes,that is,it is instable.So the prototype relaxation procedure is improved in this paper.Additionally,an immediate test of the existence of a solution following branch_and_bound is proposed,which avoids unwanted computations in those intervals that have no solution.The numerical results demonstrat that the improved interval Newton method is superior to prototype algorithm in terms of solution quality,stability and convergent speed.展开更多
The Klein-Cordon equation arises in many scientific areas of quantum mechanics and quantum field theory. In this paper a novel method based on spectral method and Jacobian free Newton method composed by generalized mi...The Klein-Cordon equation arises in many scientific areas of quantum mechanics and quantum field theory. In this paper a novel method based on spectral method and Jacobian free Newton method composed by generalized minimum residual （JFNGMRes） method with adaptive preconditioner will be introduced to solve nonlinear Klein-Gordon equation. In this work the nonlinear Klein-Gordon equation has been converted to a nonlinear system of algebraic equations using collocation method based on Bessel functions without any linearization, discretization and getting help of any other methods. Finally, by using JFNGMRes, solution of the nonlinear algebraic system will be achieved. To illustrate the reliability and efficiency of the proposed method, we solve some examples of the Klein-Gordon equation and compare our results with other methods.展开更多
基金Food & Agriculture Organization FMD Project"Progressive Control of Foot and Mouth Disease in Pakistan(GCP/PAK/123/USA)the FAO (GTFS/INT/907/ITA) and EU(SLSP) funded projects
文摘The present study describes the frequency of Foot and Mouth Disease （FMD） virus serotypes （O, A and Asia-l） in major regions （all provinces） of Pakistan using Indirect Sandwich ELISA. Also, spatial distribution of various FMD serotypes and their comparison is discussed. A total of 590 samples （Epithelial tissue） have been analyzed during a period of five years （2005-2009）. Out of 590 samples, 180 were found positive, giving an overall confirmation of FMDV about 33.2 %. Of the prevalent serotypes, FMDV ＇O＇ serotype caused most outbreaks （20.7 %）, followed by serotype A （6.6 %） and serotype Asia-1 （4.6 %） while there was no positive case of type ＇C＇. The study clearly showed that the disease was more frequent in the agro-climatic zones than in hilly areas. Based on the data of 590 samples （〉50 outbreaks）, the overall prevalence of FMDV in cattle and buffaloes in Pakistan was 33.2 %, while in cattle alone, it was 37.1%, higher than in buffalo （28.7 %）. There were eight cases of mixed serotypes infection, indicating the presence of endemic state of disease. Another significant feature was the change over time. In phase-I （2005-2007）, there was an overall prevalence of 29.4 %, while the occurrence of the serotype O, A and Asia-1 was 20.4 %, 2.9 % and 4.7 %, respectively. During phase-II （2008-2009）, the overall prevalence was 59.21%, while those of serotype O, A and Asia-1 were 22.4 %, 31.6 % and 4.0 %, respectively. This clearly indicated a shift from serotype O to A, which may help to explain the occurrence of more severe outbreaks, despite vaccination.
文摘The basic principle of interval arithmetic and the basic algorithm of the interval Newton methods are introduced.The prototype algorithm can not find any zero in an interval that has zero sometimes,that is,it is instable.So the prototype relaxation procedure is improved in this paper.Additionally,an immediate test of the existence of a solution following branch_and_bound is proposed,which avoids unwanted computations in those intervals that have no solution.The numerical results demonstrat that the improved interval Newton method is superior to prototype algorithm in terms of solution quality,stability and convergent speed.
文摘The Klein-Cordon equation arises in many scientific areas of quantum mechanics and quantum field theory. In this paper a novel method based on spectral method and Jacobian free Newton method composed by generalized minimum residual （JFNGMRes） method with adaptive preconditioner will be introduced to solve nonlinear Klein-Gordon equation. In this work the nonlinear Klein-Gordon equation has been converted to a nonlinear system of algebraic equations using collocation method based on Bessel functions without any linearization, discretization and getting help of any other methods. Finally, by using JFNGMRes, solution of the nonlinear algebraic system will be achieved. To illustrate the reliability and efficiency of the proposed method, we solve some examples of the Klein-Gordon equation and compare our results with other methods.
