Two different cDNA clones (Sscat1 and Sscat2) encoding catalase, the primary important H2O2-scavenging enzyme, were isolated from a AZap-cDNA library constructed from a 400 mmol/L NaCl-treated library of Suaeda salsa ...Two different cDNA clones (Sscat1 and Sscat2) encoding catalase, the primary important H2O2-scavenging enzyme, were isolated from a AZap-cDNA library constructed from a 400 mmol/L NaCl-treated library of Suaeda salsa ( L.) Pall aerial tissue. Sscat1 (1.7 kb) contains a full open reading frame of 492 amino acids and Sscat2 (1.1 kb) is a partial clone. BLAST analysis indicates that the two clones share 71.9% identity in nucleotide sequence and 75% identity in deduced amino acid sequence within the last 287 amino acid residues of Sscat1. Southern blotting analysis showed that Sscat1 is multicopy in S. salsa genome, while Sscat2 is a single copy gene. Northern blotting analysis showed a rapid increase in the steady-level of both genes in roots after 48 It salt treatment, but only Sscat1 was induced in salinity treated leaves. Time-course analysis carried out in leaves confirmed that Sscat1 was induced by salt stress, in contrast to Sscat2. These implied that the expression of Sscat1 and Sscat2 genes are differentially regulated in S. salsa. The activity of total catalase is dramatically increased in response to salt stress.展开更多
Through a simulation test carried out with soil columns (61.8 cm in diameter), the effect of precipitation on salt-water dynamics in soils was studied by in-situ monitoring of salt-water dynamics using soil salinity s...Through a simulation test carried out with soil columns (61.8 cm in diameter), the effect of precipitation on salt-water dynamics in soils was studied by in-situ monitoring of salt-water dynamics using soil salinity sensors and tensiometers. The results show that in the profile of whole silty loam soil, the surface runoff volume due to precipitation and the salt-leaching role of infiltrated precipitation increased with the depth of ground water; and in the profile with an intercalated bed of clay or with a thick upper layer of clay, the amount of surface runoff was greater but the salt-leaching role of precipitation was smaller than those in the profile of whole silty loam soil. In case of soil water being supplemented by precipitation, the evaporation of groundwater in the soil columns reduced, resulting in a great decline of salt accumulation from soil profile to surface soil. The effect of precipitation on the water regime of soil profile was performed via both water infiltration and water pressure transfer. The direct infiltration depth of precipitation was less than 1 m in general, but water pressure transfer could go up to groundwater surface directly.展开更多
In the Lower Cheliff Plain （northwestern of Algeria）, the waters resources are limited; the adoption of a rational approach in the management of irrigation water in the irrigated perimeter poses an inequality in the...In the Lower Cheliff Plain （northwestern of Algeria）, the waters resources are limited; the adoption of a rational approach in the management of irrigation water in the irrigated perimeter poses an inequality in the balance between supply and demand. The two surface water resources, Gargar and Merdjet Sidi Abed dams, do not satisfy the requirements of agriculture water. According to the National Office of the Irrigation and Drainage data, the quantity of allocated water is never distributed; the difference between allocated water and drop water can also exceed 20%, and then, another problem of management is that the water losses in the distribution can reach 20% again. The shortage irrigation water resource allocated has constrained the farmers to use groundwater. The chemical analysis of 56 simples to this water showed a significant chemical diversity in the compositions. There is a high salinity risk （C3 class） or very high risk （C4 class） of soil salinisation. A space chart distribution to the EC water probability to exceed 2.25 dS/m interpolated by the indicator kriging method showed that 78% of the groundwater surface presents a significant probability to exceed this limit. The average value of the SAR （sodium adsorption ratio） is lower than 10 that indicates a moderate risk of sodisation. This observation is in contradiction with the high values of the SAR measured in the soil solution. The approach of residual alkalinity （RSC） shows that a good number of drillings analyzed presents a positive sign RSC （RSC 〉 0）. This water presents a real danger of sodisation. They have a low salinity, which, for a farmer, does not present any danger.展开更多
文摘Two different cDNA clones (Sscat1 and Sscat2) encoding catalase, the primary important H2O2-scavenging enzyme, were isolated from a AZap-cDNA library constructed from a 400 mmol/L NaCl-treated library of Suaeda salsa ( L.) Pall aerial tissue. Sscat1 (1.7 kb) contains a full open reading frame of 492 amino acids and Sscat2 (1.1 kb) is a partial clone. BLAST analysis indicates that the two clones share 71.9% identity in nucleotide sequence and 75% identity in deduced amino acid sequence within the last 287 amino acid residues of Sscat1. Southern blotting analysis showed that Sscat1 is multicopy in S. salsa genome, while Sscat2 is a single copy gene. Northern blotting analysis showed a rapid increase in the steady-level of both genes in roots after 48 It salt treatment, but only Sscat1 was induced in salinity treated leaves. Time-course analysis carried out in leaves confirmed that Sscat1 was induced by salt stress, in contrast to Sscat2. These implied that the expression of Sscat1 and Sscat2 genes are differentially regulated in S. salsa. The activity of total catalase is dramatically increased in response to salt stress.
文摘Through a simulation test carried out with soil columns (61.8 cm in diameter), the effect of precipitation on salt-water dynamics in soils was studied by in-situ monitoring of salt-water dynamics using soil salinity sensors and tensiometers. The results show that in the profile of whole silty loam soil, the surface runoff volume due to precipitation and the salt-leaching role of infiltrated precipitation increased with the depth of ground water; and in the profile with an intercalated bed of clay or with a thick upper layer of clay, the amount of surface runoff was greater but the salt-leaching role of precipitation was smaller than those in the profile of whole silty loam soil. In case of soil water being supplemented by precipitation, the evaporation of groundwater in the soil columns reduced, resulting in a great decline of salt accumulation from soil profile to surface soil. The effect of precipitation on the water regime of soil profile was performed via both water infiltration and water pressure transfer. The direct infiltration depth of precipitation was less than 1 m in general, but water pressure transfer could go up to groundwater surface directly.
文摘In the Lower Cheliff Plain （northwestern of Algeria）, the waters resources are limited; the adoption of a rational approach in the management of irrigation water in the irrigated perimeter poses an inequality in the balance between supply and demand. The two surface water resources, Gargar and Merdjet Sidi Abed dams, do not satisfy the requirements of agriculture water. According to the National Office of the Irrigation and Drainage data, the quantity of allocated water is never distributed; the difference between allocated water and drop water can also exceed 20%, and then, another problem of management is that the water losses in the distribution can reach 20% again. The shortage irrigation water resource allocated has constrained the farmers to use groundwater. The chemical analysis of 56 simples to this water showed a significant chemical diversity in the compositions. There is a high salinity risk （C3 class） or very high risk （C4 class） of soil salinisation. A space chart distribution to the EC water probability to exceed 2.25 dS/m interpolated by the indicator kriging method showed that 78% of the groundwater surface presents a significant probability to exceed this limit. The average value of the SAR （sodium adsorption ratio） is lower than 10 that indicates a moderate risk of sodisation. This observation is in contradiction with the high values of the SAR measured in the soil solution. The approach of residual alkalinity （RSC） shows that a good number of drillings analyzed presents a positive sign RSC （RSC 〉 0）. This water presents a real danger of sodisation. They have a low salinity, which, for a farmer, does not present any danger.
