4种地锦属植物半致死低温及抗寒生理指标研究

Treated by freezing,1-year-old dormant branches of four Parthenocissus species were used as test materials to determine the electro-conductivities.According to Logistic equation fitting,the semi-lethal low temperatures were calculated.The result showed that the semi-lethal low temperatures and freezing tolerance of the examined species were ranked as: Parthenocissus quinquefolia(-16.63 ℃),P.tricuspidata(-10.78 ℃),P.laetecirens(-10.29 ℃),and P.henryana(-7.72 ℃).By comparing several physiological indexes including water saturation deficit,soluble sugar,soluble protein,MDA,and proline contents,it was found that after cold stressing for a whole winter the soluble sugar and proline contents were well correlated to semi-lethal low temperatures.So both of them could be used as the physiological indexes for cold tolerance identification and selection of cold-resistant variety.

地锦属野生种及栽培品种的ISSR分析鉴定

利用ISSR分子标记对9份地锦属植物材料进行基因组多态性分析,7条引物共扩增出74条带,其中多态带百分率达95.9%,所选引物能将全部供试材料区分开,并表现出很高的鉴定效率。利用NTSYSpc2.10e软件进行相似系数分析,9份地锦属植物材料的遗传相似系数在0.30～0.89之间,平均相似系数为0.517。通过扩增结果的UPGMA聚类,引入品种五叶地锦‘加引1号’与8个国内种质分开;8个国内种质又聚为5小叶型和非5小叶型两大类,与形态学的分类结果基本一致。研究表明,ISSR在地锦属种质资源分类和鉴定上具有一定的准确性,可以作为品种鉴定和新品种DUS测试的辅助工具。

地锦属植物的化学成分及药理研究进展

地锦属Parthenocissus Planch.植物含有多酚类、黄酮类、酚酸类、脂肪酸类及糖苷类等多种类型化学成分,具有抗氧化、抑菌、抗炎、抗血栓、调节血脂及降血糖等多药理活性,该属植物在药品、保健品和化妆品等方面有较大开发潜力。本文对地锦属植物化学成分和药理活性的研究进展进行系统综述,以期为该属植物的进一步研究和开发利用提供科学依据。

盐胁迫下不同地锦属植物的生理响应及耐盐性研究

以不同地锦属植物种子为材料,研究170 mmol/L NaCl胁迫下种子萌发、幼苗生长和其他生理指标的变化情况。结果显示,与对照相比,盐胁迫下各品种种子发芽率、发芽势、发芽指数和活力指数等指标均出现下降,地下部分干重较地上部分干重下降较为显著。各幼苗叶片相对电导率、丙二醛、脯氨酸、可溶性糖、可溶性蛋白均较对照显著升高,而异叶地锦、花叶地锦、绿叶地锦、五叶地锦的超氧化物歧化酶、过氧化物酶、过氧化氢酶较对照明显降低。三叶地锦在盐胁迫下其种子发芽能力、叶片渗透调节能力均较强,表现出较好的耐受性;异叶地锦受盐胁迫影响较大,对盐胁迫较为敏感;花叶地锦、绿叶地锦、五叶地锦、栓翅地锦介于二者中间,为中等耐盐型。

生态保护的功臣——地锦属植物

夏季,覆满墙面的爬山虎随处可见。每每见到这浓浓的绿色,燥热都会散去几分。其实,地锦属植物的作用远不止于此。

Subtree, TASS and an Analysis of the Genus Caragana

Subtree analysis and three area satements (TASS) procedure are used to deal with the area relationship in historical biogeography. On the basis of the taxon cladogram, the procedure could identify and eliminate the paralogy node and determine the informative subtree. The area relationships are generally illustrated in several subtrees and the consensus tree. The distribution pattern of the genus Caragana comprising about 72 species and occurring in 13 areas, was analyzed by using subtree method and TASS procedure in this study. The results showed seven subtrees representing the area relationships of section and series of the genus, and the consensus tree provided the 13 area relationships. These results are congruent with our former result using component analysis for Caragana.

五叶地锦化学成分研究

目的：研究五叶地锦Parthenocissus quinquefolia茎的化学成分。方法：采用薄层色谱,硅胶柱色谱,Sephadex LH-20凝胶柱色谱等方法进行分离纯化,使用波谱分析技术和理化常数对照等方法对得到的化合物进行结构鉴定。结果：从五叶地锦的醋酸乙酯部位分离得到9个化合物,分别为没食子酸（3,4,5-trihydroxy-benzoic acid1）,piceatannol（2）,白藜芦醇（res-veratrol3）,resveratrol trans-dehydrodimer（4）,cyphostemmin B（5）,pallidol（6）,cyphostemmin A（7）,槲皮素3-O-α-L-鼠李糖苷（quercetin-3-O-α-L-rhamnoside8）,myricetin-3-O-α-L-rhamnoside（9）。结论：化合物1,4~9均为首次从该植物中分离得到。