We report the fabrication of a highly sensitive field-effect transistor （FET） biosensor using thermally-reduced graphene oxide （TRGO） sheets functionalized with gold nanoparticle （NP）-antibody conjugates. Probe ...We report the fabrication of a highly sensitive field-effect transistor （FET） biosensor using thermally-reduced graphene oxide （TRGO） sheets functionalized with gold nanoparticle （NP）-antibody conjugates. Probe antibody was labeled on the surface of TRGO sheets through Au NPs and electrical detection of protein binding （Immunoglobulin G/IgG and anti-lmmunoglobulin G/anti-lgG） was accomplished by FET and direct current （dc） measurements. The protein binding events induced significant changes in the resistance of the TRGO sheet, which is referred to as the sensor response. The dependence of the sensor response on the TRGO base resistance in the sensor and the antibody areal density on the TRGO sheet was systematically studied, from which a correlation of the sensor response with sensor parameters was found： the sensor response was more significant with larger TRGO base resistance and higher antibody areal density. The detection limit of the novel biosensor was around the 0.2 ng/rnL level, which is among the best of,＇eported carbon nanomaterial-based protein sensors and can be further optimized by tuning the sensor structure.展开更多
Internal magnetic gradient plays a significant role in Nuclear Magnetic Resonance(NMR)measurements of fluid saturated porous media.The quantitative characterization and application of this physical phenomenon could ef...Internal magnetic gradient plays a significant role in Nuclear Magnetic Resonance(NMR)measurements of fluid saturated porous media.The quantitative characterization and application of this physical phenomenon could effectively improve the accuracy of NMR measurements and interpretations.In this paper,by using the equivalent magnetic dipole method,the three-dimensional distribution of internal induced magnetic field and its gradients in the randomly packed water saturated glass beads are quantitatively characterized.By simulating the diffusive motion of water molecules in porous media with random walk method,the computational dephasing effects equation related to internal gradients is deduced.Thereafter,the echo amplitudes are obtained and the corresponding T2-G spectrum is also inverted.For the sake of verifying the simulation results,an experiment is carried out using the Halbach core analyzing system(B0=0.18 T,G=2.3 T/m)to detect the induced internal field and gradients.The simulation results indicate the equivalent internal gradient is a distribution of 0.1-0.3 T/m,which matched well with the experimental results.展开更多
基金Financial support for this work was provided by the USA National Science Foundation （NSF） （Nos. CMMI- 0900509, CBET-0803142, and ECCS-0708998）. Graphene oxide samples were supplied by Prof. Rodney S. Ruoff. The authors thank Dr. Heather A. Owen for technical support with SEM, and Dr. Leonidas E. Ocola for assistance in the electrode fabrication. The e-beam lithography was performed at the Center for Nanoscale Materials of Argonne National Laboratory, which is supported by the USA Department of Energy （No. DE- AC02-06CH11357）. The SEM imaging was conducted at the Electron Microscope Laboratory of University of Wisconsin-Milwaukee.
文摘We report the fabrication of a highly sensitive field-effect transistor （FET） biosensor using thermally-reduced graphene oxide （TRGO） sheets functionalized with gold nanoparticle （NP）-antibody conjugates. Probe antibody was labeled on the surface of TRGO sheets through Au NPs and electrical detection of protein binding （Immunoglobulin G/IgG and anti-lmmunoglobulin G/anti-lgG） was accomplished by FET and direct current （dc） measurements. The protein binding events induced significant changes in the resistance of the TRGO sheet, which is referred to as the sensor response. The dependence of the sensor response on the TRGO base resistance in the sensor and the antibody areal density on the TRGO sheet was systematically studied, from which a correlation of the sensor response with sensor parameters was found： the sensor response was more significant with larger TRGO base resistance and higher antibody areal density. The detection limit of the novel biosensor was around the 0.2 ng/rnL level, which is among the best of,＇eported carbon nanomaterial-based protein sensors and can be further optimized by tuning the sensor structure.
基金supported by the National Natural Science Foundation of China(Grant Nos.41074102 and 41130417)"111 Program"(Grant No.B13010)Program for Changjiang Scholars and Innovative Research Team in University
文摘Internal magnetic gradient plays a significant role in Nuclear Magnetic Resonance(NMR)measurements of fluid saturated porous media.The quantitative characterization and application of this physical phenomenon could effectively improve the accuracy of NMR measurements and interpretations.In this paper,by using the equivalent magnetic dipole method,the three-dimensional distribution of internal induced magnetic field and its gradients in the randomly packed water saturated glass beads are quantitatively characterized.By simulating the diffusive motion of water molecules in porous media with random walk method,the computational dephasing effects equation related to internal gradients is deduced.Thereafter,the echo amplitudes are obtained and the corresponding T2-G spectrum is also inverted.For the sake of verifying the simulation results,an experiment is carried out using the Halbach core analyzing system(B0=0.18 T,G=2.3 T/m)to detect the induced internal field and gradients.The simulation results indicate the equivalent internal gradient is a distribution of 0.1-0.3 T/m,which matched well with the experimental results.
