The study was conducted to investigate the effects of feeding high-fiber diet （HFD） on apparent total tract digestibility （ATTD） and absorption of minerals in growing pigs. Sixteen barrows averaging 16.39 kg were ...The study was conducted to investigate the effects of feeding high-fiber diet （HFD） on apparent total tract digestibility （ATTD） and absorption of minerals in growing pigs. Sixteen barrows averaging 16.39 kg were randomly assigned to one of four diets after metabolic crate assignment with individual pig serving as experimental unit. Diets include： a corn-soybean meal based low-fiber diet （LFD）, as the control （CON） and 3 HFD （D1, D2 and D3）, each containing 5% sugar beet pulps and a 1：2, 1：1 and 2：1 oats to barley ratios, respectively, replacing 47% of the corn in the diet. Diets were formulated to meet nutrient requirements, however, the fiber content of the LFD was substantially lower than the average of the HFD （8.38% vs. 22.40%）. Feed allowance was limited to 10% of average body weight and fed twice/d. The study period was divided into 10 d of adjustment and 5 d of feces collection. Data collected were analyzed using SAS, GLIMMIX procedure where animals were considered random effects, and minerals were fixed. Results of the study show that digestibility of Ca in pigs fed HFD was lower （P 〈 0.05） than in pigs fed LFD. On the other hand, the apparent digestibility of P, K, Na, S and Zn in pigs fed D2 and D3 were higher （P 〈 0.05） than in pigs fed CON diet. It was also observed that digestibility of Ca, P, K, Na, S and Zn in pigs fed D1 were lower （P 〈 0.05） than in CON, D2 or D3 fed pigs. Therefore, it was concluded that while feeding HFD reduced availability of Ca, the availability of P, K, Na and S to pigs improved as the oat to barley ratio in the diet increased.展开更多
10-Hydroxycamptothecin （HCPT） is a broad-spectrum anticancer drug, while its low solubility and instability severely limit its application. In this study, HCPT nanosuspension （HCPT-NSP）, also known as nanocrystal,...10-Hydroxycamptothecin （HCPT） is a broad-spectrum anticancer drug, while its low solubility and instability severely limit its application. In this study, HCPT nanosuspension （HCPT-NSP）, also known as nanocrystal, was prepared by micro-precipitation combined with high-pressure homogenization method. This nanosuspension was characterized by size, shape, zeta potential, drug loading efficiency and in vitro drug release behavior. Preferred formulation and process showed that particle size was （129.8±13.9） nm, PDI was 0.20±0.07, and drug loading efficiency was 36.5%±9.5%. Moreover, HCPT nanocrystal concentration reached （1.35±0.2） mg/mL in HCPT-NSP, which was more than 1000-fold higher than that of HCPT. Transmission electron microscopy （TEM） results showed that the nanosuspension was short rod in shape. X-ray powder diffraction （XRD）, thermogravimetric analysis （TGA）, derivative thermogravimetric analysis （DTA） and differential scanning calorimetry （DSC） further elaborated the crystal state of the HCPT. The drug concentration-time curve of HCPT-NSP in rats was in accordance with the three-compartment model, showing prolonged half-life. Taken together, our data suggested that HCPT-NSP was a promising drug delivery system.展开更多
Total paeony glycoside（TPG） is obtained from Radix Paeoniae Rubra with a variety of bioactivities. However, the low solubility and bioavailability limit its application. The present study aimed to develop TPG nanocr...Total paeony glycoside（TPG） is obtained from Radix Paeoniae Rubra with a variety of bioactivities. However, the low solubility and bioavailability limit its application. The present study aimed to develop TPG nanocrystals to increase the dissolution and then improve the oral bioavailability. TPG nanocrystals were prepared via precipitation and high-pressure homogenization method. The physical-chemical properties of the optimal TPG nanocrystals in terms of particle size, zeta potential, morphology and crystallinity were evaluated. The results showed that TPG nanocrystals had a mean particle size of（210.2±2.5） nm, a polydispersity index of 0.191±0.033 and a zeta potential of（–22.4±1.2） mV. The result of differential scanning calorimetry showed that the nanocrystals were still in crystalline state after the preparation procedure. Transmission electron microscopy（TEM） results showed that the nanosuspension was in spherical shape. The pharmacokinetics of TPG nanocrystals for rats was investigated by liquid chromatography-tandem mass spectroscopy（LC-MS/MS）. Compared with the TPG coarse suspension, TPG nanocrystals exhibited significant increase in AUC0–∞（approximately 1.85-fold）. Taken together, TPG nanocrystals could be used as a promising drug delivery system due to the enhanced oral bioavailability of TPG.展开更多
文摘The study was conducted to investigate the effects of feeding high-fiber diet （HFD） on apparent total tract digestibility （ATTD） and absorption of minerals in growing pigs. Sixteen barrows averaging 16.39 kg were randomly assigned to one of four diets after metabolic crate assignment with individual pig serving as experimental unit. Diets include： a corn-soybean meal based low-fiber diet （LFD）, as the control （CON） and 3 HFD （D1, D2 and D3）, each containing 5% sugar beet pulps and a 1：2, 1：1 and 2：1 oats to barley ratios, respectively, replacing 47% of the corn in the diet. Diets were formulated to meet nutrient requirements, however, the fiber content of the LFD was substantially lower than the average of the HFD （8.38% vs. 22.40%）. Feed allowance was limited to 10% of average body weight and fed twice/d. The study period was divided into 10 d of adjustment and 5 d of feces collection. Data collected were analyzed using SAS, GLIMMIX procedure where animals were considered random effects, and minerals were fixed. Results of the study show that digestibility of Ca in pigs fed HFD was lower （P 〈 0.05） than in pigs fed LFD. On the other hand, the apparent digestibility of P, K, Na, S and Zn in pigs fed D2 and D3 were higher （P 〈 0.05） than in pigs fed CON diet. It was also observed that digestibility of Ca, P, K, Na, S and Zn in pigs fed D1 were lower （P 〈 0.05） than in CON, D2 or D3 fed pigs. Therefore, it was concluded that while feeding HFD reduced availability of Ca, the availability of P, K, Na and S to pigs improved as the oat to barley ratio in the diet increased.
基金NSFC(Grant No.81473156,81673365)Fangzheng Foundation for funding of the work
文摘10-Hydroxycamptothecin （HCPT） is a broad-spectrum anticancer drug, while its low solubility and instability severely limit its application. In this study, HCPT nanosuspension （HCPT-NSP）, also known as nanocrystal, was prepared by micro-precipitation combined with high-pressure homogenization method. This nanosuspension was characterized by size, shape, zeta potential, drug loading efficiency and in vitro drug release behavior. Preferred formulation and process showed that particle size was （129.8±13.9） nm, PDI was 0.20±0.07, and drug loading efficiency was 36.5%±9.5%. Moreover, HCPT nanocrystal concentration reached （1.35±0.2） mg/mL in HCPT-NSP, which was more than 1000-fold higher than that of HCPT. Transmission electron microscopy （TEM） results showed that the nanosuspension was short rod in shape. X-ray powder diffraction （XRD）, thermogravimetric analysis （TGA）, derivative thermogravimetric analysis （DTA） and differential scanning calorimetry （DSC） further elaborated the crystal state of the HCPT. The drug concentration-time curve of HCPT-NSP in rats was in accordance with the three-compartment model, showing prolonged half-life. Taken together, our data suggested that HCPT-NSP was a promising drug delivery system.
基金Innovation Team Project(Grant No.LT2015011)from the Education Department of Liaoning ProvinceImportant Sci entific and Technical Achievements Transformation Project(Gr ant No.Z17-5-078)+1 种基金Applied Basic Research Project(Grant No.F16205144)of Science and Technology Bureau of Shenyangthe Liaoning Provincial Education Department Project of China(Grant No.L2015192)
文摘Total paeony glycoside（TPG） is obtained from Radix Paeoniae Rubra with a variety of bioactivities. However, the low solubility and bioavailability limit its application. The present study aimed to develop TPG nanocrystals to increase the dissolution and then improve the oral bioavailability. TPG nanocrystals were prepared via precipitation and high-pressure homogenization method. The physical-chemical properties of the optimal TPG nanocrystals in terms of particle size, zeta potential, morphology and crystallinity were evaluated. The results showed that TPG nanocrystals had a mean particle size of（210.2±2.5） nm, a polydispersity index of 0.191±0.033 and a zeta potential of（–22.4±1.2） mV. The result of differential scanning calorimetry showed that the nanocrystals were still in crystalline state after the preparation procedure. Transmission electron microscopy（TEM） results showed that the nanosuspension was in spherical shape. The pharmacokinetics of TPG nanocrystals for rats was investigated by liquid chromatography-tandem mass spectroscopy（LC-MS/MS）. Compared with the TPG coarse suspension, TPG nanocrystals exhibited significant increase in AUC0–∞（approximately 1.85-fold）. Taken together, TPG nanocrystals could be used as a promising drug delivery system due to the enhanced oral bioavailability of TPG.
