Objective To observe the influence of acupuncture on hypothalamus-pituitary-ovary axis （HPOA） in rats with primary dysmenorrhea, and to explore its mechanisms. Methods Effects of acupuncture on the concentration lev...Objective To observe the influence of acupuncture on hypothalamus-pituitary-ovary axis （HPOA） in rats with primary dysmenorrhea, and to explore its mechanisms. Methods Effects of acupuncture on the concentration level of β-endorphin （β-EP）, hypothalamic hormone （gonadotropin-releasing hormone, GnRH）, pituitary hormone （GnRH receptor GnRH-R, follicle stimulating hormone FSH, luteinizing hormone LH） and ovarian hormone （estrodiol E2, estrodiol receptor ER, progesterone P and progesterone receptor PR） were observed in dysmenorrheic rats. Results After receiving acupuncture treatment, the levels of 13- EP, GnRH, GnRH-R, FSH, LH, E2, ER, P and PR were changed, and this suggested that acupuncture had a regulative action on HPOA in dysmenorrheic rats. Conclusion The mechanism of acupuncture acting on the HPOA with primary dysmenorrhea might be its regulating role to the gonadal hormone of HPOA and its corresponding receptor expression.展开更多
Objective： To investigate the neurobiological mechanism of depression pathogenesis and reveal the mechanism of acupuncture treatment of depression. Methods： Wistar rats were selected for subjects. A rat model of dep...Objective： To investigate the neurobiological mechanism of depression pathogenesis and reveal the mechanism of acupuncture treatment of depression. Methods： Wistar rats were selected for subjects. A rat model of depression was made by individually housing with unpredicted chronic moderate stimuli. Changes in behavior and hypothalamus-pituitaryadrenocortical axis were examined in rat models of stress-induced depression. Meanwhile, the intervening effect of acupuncture was evaluated and the curative effects of different acupuncture methods compared. Results： CORT and ACTH contents of serum were significantly higher in the model and normal saline groups than in the control group （P〈0.05）, significantly lower in the hand acupuncture and electroacupuncture groups than in the model group （P〈0.05） and significantly lower in the medication group than in the normal saline group （P〈0.05）. There were no significant differences between the hand acupuncture, electroacupuncture and medication groups. Conclusion： Acupuncture of Baihui（GV 20） and Taichong （LR 3） has a marked antidepressant effect. Its mechanism may be related to the regulation of HPA axis by acupuncture.展开更多
OBJECTIVE: To investigate the antidepressant mechanism of acupuncture at the molecular level in the pituitary gland of rats exposed to chronic restraint stress(CRS).METHODS: Forty-eight rats were randomly divided into...OBJECTIVE: To investigate the antidepressant mechanism of acupuncture at the molecular level in the pituitary gland of rats exposed to chronic restraint stress(CRS).METHODS: Forty-eight rats were randomly divided into a control group(C), model group(M), fluoxetine group(F), and acupuncture group(A). CRS was induced in the model group by restraining rats for 28 days. Groups F and A were given fluoxetine administration and acupuncture intervention, respectively, 1 h before the CRS procedures were implemented. On the 29 th day, rat pituitary glands were harvested for RNA extraction and RNA-sequencing analysis.RESULTS: Differentially expressed gene analysis identified 148, 87, and 179 response genes differen-tially expressed in group C vs group M, group F vs group M, and group A vs group M, respectively. Using Gene Ontology term enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis, we identified the up-regulation of gene sets involved in extracellular space,receptor binding, and monoamine neurotransmitter metabolism, and the down-regulation of gene sets involved in immune response and inflammatory response as the prevailing transcriptomic signatures in the pituitary gland of rats treated with CRS,fluoxetine, or acupuncture.CONCLUSION: Our findings suggest that acupuncture has a multitarget antidepressant effect at the molecular and behavioral levels.展开更多
基金National Basic Research Program of China(973 program,No:2006CB504502)Chinese Na-tional Foundation of Natural Sciences.(No.90209009)
文摘Objective To observe the influence of acupuncture on hypothalamus-pituitary-ovary axis （HPOA） in rats with primary dysmenorrhea, and to explore its mechanisms. Methods Effects of acupuncture on the concentration level of β-endorphin （β-EP）, hypothalamic hormone （gonadotropin-releasing hormone, GnRH）, pituitary hormone （GnRH receptor GnRH-R, follicle stimulating hormone FSH, luteinizing hormone LH） and ovarian hormone （estrodiol E2, estrodiol receptor ER, progesterone P and progesterone receptor PR） were observed in dysmenorrheic rats. Results After receiving acupuncture treatment, the levels of 13- EP, GnRH, GnRH-R, FSH, LH, E2, ER, P and PR were changed, and this suggested that acupuncture had a regulative action on HPOA in dysmenorrheic rats. Conclusion The mechanism of acupuncture acting on the HPOA with primary dysmenorrhea might be its regulating role to the gonadal hormone of HPOA and its corresponding receptor expression.
文摘Objective： To investigate the neurobiological mechanism of depression pathogenesis and reveal the mechanism of acupuncture treatment of depression. Methods： Wistar rats were selected for subjects. A rat model of depression was made by individually housing with unpredicted chronic moderate stimuli. Changes in behavior and hypothalamus-pituitaryadrenocortical axis were examined in rat models of stress-induced depression. Meanwhile, the intervening effect of acupuncture was evaluated and the curative effects of different acupuncture methods compared. Results： CORT and ACTH contents of serum were significantly higher in the model and normal saline groups than in the control group （P〈0.05）, significantly lower in the hand acupuncture and electroacupuncture groups than in the model group （P〈0.05） and significantly lower in the medication group than in the normal saline group （P〈0.05）. There were no significant differences between the hand acupuncture, electroacupuncture and medication groups. Conclusion： Acupuncture of Baihui（GV 20） and Taichong （LR 3） has a marked antidepressant effect. Its mechanism may be related to the regulation of HPA axis by acupuncture.
基金Supported by the National Natural Science Foundation of China(No.81373729)the Outstanding doctoral dissertation guidance teachers of science and technology projects of Beijing(No.YB20091002601)
文摘OBJECTIVE: To investigate the antidepressant mechanism of acupuncture at the molecular level in the pituitary gland of rats exposed to chronic restraint stress(CRS).METHODS: Forty-eight rats were randomly divided into a control group(C), model group(M), fluoxetine group(F), and acupuncture group(A). CRS was induced in the model group by restraining rats for 28 days. Groups F and A were given fluoxetine administration and acupuncture intervention, respectively, 1 h before the CRS procedures were implemented. On the 29 th day, rat pituitary glands were harvested for RNA extraction and RNA-sequencing analysis.RESULTS: Differentially expressed gene analysis identified 148, 87, and 179 response genes differen-tially expressed in group C vs group M, group F vs group M, and group A vs group M, respectively. Using Gene Ontology term enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis, we identified the up-regulation of gene sets involved in extracellular space,receptor binding, and monoamine neurotransmitter metabolism, and the down-regulation of gene sets involved in immune response and inflammatory response as the prevailing transcriptomic signatures in the pituitary gland of rats treated with CRS,fluoxetine, or acupuncture.CONCLUSION: Our findings suggest that acupuncture has a multitarget antidepressant effect at the molecular and behavioral levels.
