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鸡新城疫疫苗点眼免疫后哈氏腺中IgA、IgM、IgG型浆细胞的数量变化及分布特点 被引量:2
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作者 王彩虹 高齐瑜 孙斌 《畜牧兽医学报》 CAS CSCD 北大核心 2002年第5期504-507,共4页
用Lasota系ND疫苗分别于 10、2 0日龄进行点眼免疫后采用SLAB免疫组织化学方法对鸡哈氏腺在鸡新城疫 (NewcastleDisease ,ND)疫苗点眼免疫后IgA、IgM、IgG型浆细胞的数量变化及分布特征进行了研究。研究发现 ,Lasota系ND疫苗点眼免疫后... 用Lasota系ND疫苗分别于 10、2 0日龄进行点眼免疫后采用SLAB免疫组织化学方法对鸡哈氏腺在鸡新城疫 (NewcastleDisease ,ND)疫苗点眼免疫后IgA、IgM、IgG型浆细胞的数量变化及分布特征进行了研究。研究发现 ,Lasota系ND疫苗点眼免疫后鸡哈氏腺中 (HarderianGland ,HG)中IgA、IgM、IgG型浆细胞的出现较对照组早 ,并于 48日龄达到峰值 ,一免及二免后三种类型的浆细胞数量均极显著高于对照组 (P <0 0 1) ,其中IgA型浆细胞的数量最多 ,IgM型浆细胞次之 ,IgG型浆细胞的数量最少。IgA、IgM、IgG型三种浆细胞均散在分布于哈氏腺的间质中 ,且主要围绕各级管腔上皮及腺上皮分布 ,其中成熟的浆细胞紧贴腺上皮及管腔上皮分布。表明Lasota系ND疫苗点眼免疫后可促进哈氏腺中的IgA、IgM、IgG型浆细胞的分化、成熟 ,这些浆细胞分泌的抗体成分可通过上皮而被分泌到管腔中去 ,最后到达泪液中 ,在眼区及上呼吸道起到保护作用。 展开更多
关键词 鸡新 城疫疫苗 点眼免 哈氏腺 IgA型浆细胞 IgM型浆细胞 IgG型浆细胞 数量变化 分布特点
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Effects of Three Kinds of Chinese Medicine Polysaccharides on the Immune Effect of Newcastle Disease Vaccine 被引量:7
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作者 杨庆芳 杨康林 +1 位作者 姚静 蒋春茂 《Agricultural Science & Technology》 CAS 2015年第10期2283-2285,2303,共4页
240 14-day-old healthy and non-immune Roman chicken were randomly divided into 8 groups, including blank control group (BC group), immune control group (IC group), and immunity groups of each Chinese medicine. On ... 240 14-day-old healthy and non-immune Roman chicken were randomly divided into 8 groups, including blank control group (BC group), immune control group (IC group), and immunity groups of each Chinese medicine. On the day of the first immunity, 3 d before the second immunity, the day of the second immunity and 3 d after the second immunity, high-dose concentration and low-dose concen- tration of Astragalus polysaccharide (ASP), Epimedium polysaccharide (EPP) and Isatis roots polysaccharide (IRPS) were used for the immunity groups of each Chi- nese medicine using the gavage, and 0.5 ml for each chick, and the equivalent normal saline was used for the blank control group and vaccine control group. On the 7^th, 14^th, 21^st and 28^th day after the first immunity, 10 chicken of each group were randomly got and weighed, and the antibody titer and the changes of the pro- liferation of T lymphocyte were measured. The results showed that 3 kinds of Chi- nese medicine polysaccharides all can increase the weight of chicken, improve HI antibody titer of Newcastle disease, and promote the proliferation of peripheral T lymphocyte, in which the effect of IRPS at low dosage is the best. 展开更多
关键词 Astragalus polysaccharides (ASP) Epimedium polysaccharides (EPP) Isatis root polysaccharides (IRPS) Newcastle disease vaccine Antibody titer T lymphocyte
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Study on Inactivated Vaccine in Oil Emulsion Against Newcastle Disease and Fowl Cholera IV——Field Test of Vaccine 被引量:5
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作者 王红琳 杨峻 +6 位作者 邵华斌 温国元 艾地云 罗玲 罗青平 张蓉蓉 张琳 《Agricultural Science & Technology》 CAS 2009年第2期102-107,共6页
[ Objective] The aim of this study was to evaluate the clinical effect of the inactivated vaccine in oil emulsion against Newcastle disease and Fowl cholera, and provide conditions for combined prevention and control ... [ Objective] The aim of this study was to evaluate the clinical effect of the inactivated vaccine in oil emulsion against Newcastle disease and Fowl cholera, and provide conditions for combined prevention and control of Newcastle disease and Fowl cholera. [ Method] The mixture of avian pasteurella multocida (type A) virulent strain 1502 and Newcastle disease virus attenuated strain La Sota was prepared into five batches of the inactivated vaccine in oil emulsion to use in the field test for assessing its safety and effects on immune protection of chicken, duck and goose. [ Result] The field safety test showed that there was no adverse reaction in the vaccinated chickens, ducks and geese. The field test of immune effect for chickens suggested that the titers of hemagglutination inhibition antibody for Nescastle disease virus ( ND-HI ) in 7 - 14 day- old chickens and 60 -90 day-old young chickens were 2 -3 log2 higher than the control group after being vaccinated for 3 weeks, which could last for more than 4 months. The protection rate against avian pasteurella multocida was over 75.0% and its immune effect could last for 6 months. The field test of immune effect for duck and goose indicated that the titers of ND-HI antibody were all higher than 4.2 log2 in vaccinated ducks and geese while lower than 2 log2 in the control group after being vaccinated for 3 weeks. The protection rate against avian pasteurella multocida in vaccinated ducks and geese was higher than 75.0% and 62.5% respectively. [ Conclusion] The binary vaccine is safe for poultry and has good immune effects. 展开更多
关键词 Fowl cholera Newcastle disease Binary vaccine Field test
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Immunological Adjuvant Function of Aluminium Phosphate and Chicken IL-18 in NDV F Gene Vaccine
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作者 尹会方 黄青云 +2 位作者 王继文 何英杰 吴思源 《Agricultural Science & Technology》 CAS 2012年第12期2581-2584,2619,共5页
[Objective] This study aimed to investigate the immunological adjuvant function of aluminium phosphate and chicken IL-18 in NDV F gene vaccine. [Method] The vaccine (0.2 ml) containing aluminum phosphate adjuvant (... [Objective] This study aimed to investigate the immunological adjuvant function of aluminium phosphate and chicken IL-18 in NDV F gene vaccine. [Method] The vaccine (0.2 ml) containing aluminum phosphate adjuvant (90 μg), pcDNA/F (200μg), and pcDNA/chlL-18 (200 μg) was prepared. The 7 d old chick- ens to be tested were randomly divided into six groups (12 chickens in each group) and immunized through intramuscular injection with inactivated Newcastle disease vaccines, pcDNA/F+pcDNA/chlL-18+phosphate aluminum, pcDNA/F, pcDNA/F.+pcDNA/ chlL-18, pcDNA/F+aluminum phosphate, and physiological saline respectively; the secondary immunization was conducted with the same dose when the chickens were 21 d old. Their blood was sampled 0, 7, 14, 21, 28 d after first immunization. Anti- body titer was detected with ELISA and T cell transformation rate was measured with MIT. Experimental chicken will be challenged with 30 LD50 NDV virulence 28 d after first immunization. [Result] The survival rate of the chickens immunized with pcDNA/F+aluminium phosphate+pcDNA/chlL-18 achieved 8/12, higher than that of those immunized with pcDNA/F 4/12 and pcDNA/F+pcDNA/chlL-18 (6/12). The NDV antibody titer of the chickens immunized with pcDNA/F+ aluminum phosphate, pcD- NA/F+pcDNA/chlL-18 and pcDNA/F+pcDNA/chlL-18+aluminum phosphate is not differ- ent (P〉0.05), but significantly lower than that of the chickens immunized with tradi- tional vaccine (P〈0.05). The T cell transformation rate of the chickens immunized with pcDNA/F+pcDNA/chlL-18+aluminium phosphate was obviously higher than that of the chickens immunized with pcDNA/F (P〈0.05). The T cell transformation rates of chickens immunized with pcDNA/F and the traditional vaccine showed no signifi- cant difference (P〉0.05). [Conclusion] Combination of aluminium phosphate and pcD- NA/chlL-18 can significantly enhance the immune effect of NDV F gene vaccine. 展开更多
关键词 Immunological adjuvant: Aluminium phosphate Chicken IL-18 NDV F gene Gene vaccine
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