目的进行血培养单、双侧采血阳性率比较及病原菌分布和耐药性分析。方法分析第一时间段(2006年6月至2007年4月)单侧采血及第二时间段(2007年6月至2008年4月)双侧采血的阳性率及耐药菌的检出率,采用全自动血培养仪BacT/ALERT 3D Select...目的进行血培养单、双侧采血阳性率比较及病原菌分布和耐药性分析。方法分析第一时间段(2006年6月至2007年4月)单侧采血及第二时间段(2007年6月至2008年4月)双侧采血的阳性率及耐药菌的检出率,采用全自动血培养仪BacT/ALERT 3D Select系统进行血培养。阳性血培养转种后进行常规细菌鉴定,药敏试验采用纸片扩散法。结果第二时间段双侧采血的血培养阳性率为11.12%,比第一时间段单侧采血的血培养阳性率6.04%高出5.08%;耐甲氧西林金黄色葡萄球菌(MRSA)检出率:第一时间段为33.3%,第二时间段为36.4%;耐甲氧西林凝固酶阴性葡萄球菌(MRCNS)检出率:第一时间段为55.5%,第二时间段为70.8%;产超广谱β-内酰胺酶(ESBLs)检出率:第一时间段为24.0%,第二时间段为50.0%。结论双侧采血比单侧采血阳性率高,MRSA、MRCNS及产ESBLs细菌检出率上升较快。展开更多
[Objective] Brevibacil us laterosporus BL-21 was inhibitory to Gibberel a sanbinetti, Fusarium graminearum Magnaporthe grisea and many other phy-topathogenic fungi. Antimicrobial substances can be increased through op...[Objective] Brevibacil us laterosporus BL-21 was inhibitory to Gibberel a sanbinetti, Fusarium graminearum Magnaporthe grisea and many other phy-topathogenic fungi. Antimicrobial substances can be increased through optimizing the medium of Brevibacil us laterosporus. [Method] A total of 10 factors were tested for their function of improving antibiotics yield of BL-21 by single-factor test. And the indicative bacterium was screened. [Result] The best indicative bacterium was Al-ternaria solani, the optimum process parameters in shaking flask on fermentation consisted of initial pH of 8.0, culture at 30 ℃, loadage 90 ml/250 ml, rotation 180 r/min, and fermentation time of 48 h. The results also indicated that glucose was the best carbon source for the antibiotics productivity of BL-21, and beef extract was the best nitrogen source. In addition, univalent ion Cl＋ and divalent ion Mn2＋ stimu-lated BL-21 antibacterial active component biosynthesis. [Conclusion] Optimized medi-um is more conducive to produce antimicrobial substances.展开更多
文摘目的进行血培养单、双侧采血阳性率比较及病原菌分布和耐药性分析。方法分析第一时间段(2006年6月至2007年4月)单侧采血及第二时间段(2007年6月至2008年4月)双侧采血的阳性率及耐药菌的检出率,采用全自动血培养仪BacT/ALERT 3D Select系统进行血培养。阳性血培养转种后进行常规细菌鉴定,药敏试验采用纸片扩散法。结果第二时间段双侧采血的血培养阳性率为11.12%,比第一时间段单侧采血的血培养阳性率6.04%高出5.08%;耐甲氧西林金黄色葡萄球菌(MRSA)检出率:第一时间段为33.3%,第二时间段为36.4%;耐甲氧西林凝固酶阴性葡萄球菌(MRCNS)检出率:第一时间段为55.5%,第二时间段为70.8%;产超广谱β-内酰胺酶(ESBLs)检出率:第一时间段为24.0%,第二时间段为50.0%。结论双侧采血比单侧采血阳性率高,MRSA、MRCNS及产ESBLs细菌检出率上升较快。
基金Supported by National Natural Science Foundation of China(31370511)Natural Science Foundation of Heilongjiang Province(C201203)Open Fund of Key Laboratory of Microbiology of Heilongjiang University(2012MOI-1)~~
文摘[Objective] Brevibacil us laterosporus BL-21 was inhibitory to Gibberel a sanbinetti, Fusarium graminearum Magnaporthe grisea and many other phy-topathogenic fungi. Antimicrobial substances can be increased through optimizing the medium of Brevibacil us laterosporus. [Method] A total of 10 factors were tested for their function of improving antibiotics yield of BL-21 by single-factor test. And the indicative bacterium was screened. [Result] The best indicative bacterium was Al-ternaria solani, the optimum process parameters in shaking flask on fermentation consisted of initial pH of 8.0, culture at 30 ℃, loadage 90 ml/250 ml, rotation 180 r/min, and fermentation time of 48 h. The results also indicated that glucose was the best carbon source for the antibiotics productivity of BL-21, and beef extract was the best nitrogen source. In addition, univalent ion Cl＋ and divalent ion Mn2＋ stimu-lated BL-21 antibacterial active component biosynthesis. [Conclusion] Optimized medi-um is more conducive to produce antimicrobial substances.
