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一种秸秆生化培养剂发酵稻草饲喂肉牛消化率及消化能的研究 被引量:1
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作者 刘禹宏 宋金昌 +5 位作者 韩淑珍 刘锁才 邢光华 张守义 程立君 李淑玲 《粮食与饲料工业》 CAS 北大核心 1999年第5期34-35,共2页
采用一种生化及生物活性物的复合制剂,用它发酵的秸秆不需外加人工培养菌株,靠天然有益菌的大量繁殖来增加菌体蛋白,并有抑制杂菌生长,降解粗纤维的作用,能够大大提高秸秆的有效养分和生物学价值。用该生化培养剂处理的稻草,蛋白... 采用一种生化及生物活性物的复合制剂,用它发酵的秸秆不需外加人工培养菌株,靠天然有益菌的大量繁殖来增加菌体蛋白,并有抑制杂菌生长,降解粗纤维的作用,能够大大提高秸秆的有效养分和生物学价值。用该生化培养剂处理的稻草,蛋白质可提高25~35个百分点,粗纤维可降低12~16个百分点。以秸秆生化培养剂发酵稻草7086%、发酵脱毒棉籽饼1227%,大豆饼1534%、食盐15%组成饲粮,饲喂舍饲肥育肉牛,杂交肥育肉牛日增重达到1500~1833kg,增重料比达356~435。 展开更多
关键词 秸秆 生化培养剂 牛饲料 肉牛 饲料
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秸秆生化培养剂发酵稻草饲喂肉牛及其安全性试验
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作者 刘禹宏 韩淑珍 +3 位作者 邢光华 张守义 程立君 李叔玲 《中国饲料》 北大核心 1998年第12期15-17,共3页
秸秆生化培养剂是由河北省科委下达、由秦皇岛耀华集团承担的“秸秆生化培养剂及其无粮饲料研究”课题的新产品,该生化培训剂是一种化工、生化及生物活性物质的复合物。
关键词 肉牛 秸秆生化培养剂 发酵稻草 饲喂 安全性
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秸秆生化培养剂发酵稻草安全性的试验研究
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作者 刘禹宏 《广西农业生物科学》 CSCD 1999年第3期197-199,共3页
本研究结果表明:急性毒性试验一次投灌剂量达到了国际认定的“食品毒性六级分级标准”规定的实际无毒级(2级)。在亚急性毒性试验中,各组的血液生理参数、红、白细计数,血红素含量均正常;血清尿素氮、肌酐、谷—丙转氨酶,直接及... 本研究结果表明:急性毒性试验一次投灌剂量达到了国际认定的“食品毒性六级分级标准”规定的实际无毒级(2级)。在亚急性毒性试验中,各组的血液生理参数、红、白细计数,血红素含量均正常;血清尿素氮、肌酐、谷—丙转氨酶,直接及间接胆红素、球蛋白、白蛋白等血液生化参数均在正常值范围。大体剖解未见异常病变。采取心、肝、脾、肺、肾、淋巴结、园小囊、隐突、大肠、小肠等脏器,用10%的甲醛固定,石蜡切片,H·E染色及Cajal硝酸银染色,显微镜下观察,未见异常组织病变。 展开更多
关键词 秸秆 生化培养剂 安全性 发酵稻草
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饲喂秸杆生化培养剂发酵稻草对牛消化率及消化能的影响
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作者 刘禹宏 《内蒙古民族大学学报》 1998年第4期14-17,21,共5页
以70.86%秸杆生化培养剂发酵稻草、12.27%发酵脱毒棉籽饼、15.34%大豆饼、1.5%食盐组成饲粮,饲喂舍饲肥育肉牛,结果表明;该饲粮的干物质中粗蛋白质消化率为61.46-66.55%,粗纤维消化率44.05—52.02%,每千克饲粮DE为2... 以70.86%秸杆生化培养剂发酵稻草、12.27%发酵脱毒棉籽饼、15.34%大豆饼、1.5%食盐组成饲粮,饲喂舍饲肥育肉牛,结果表明;该饲粮的干物质中粗蛋白质消化率为61.46-66.55%,粗纤维消化率44.05—52.02%,每千克饲粮DE为2.12Mcal,杂交肥育肉牛日增重达到1.500—1.833千克,增重料比达3.56—4.35。 展开更多
关键词 秸杆 生化培养剂 消化率 消化能
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新型秸秆——生化培养剂
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作者 刘禹宏 《中国农村小康科技》 2002年第9期27-27,共1页
农作物秸秆无疑是反刍家畜的重要粗饲料源,但我国有关秸秆养牛的实践大多都忽视了秸秆饲料的综合处理、组合利用、营养补充和草食畜各饲料源的互作效应,以调节动体“消化环境”,使秸秆的利用还存在着一定的缺憾。 一、新型秸秆生化剂的... 农作物秸秆无疑是反刍家畜的重要粗饲料源,但我国有关秸秆养牛的实践大多都忽视了秸秆饲料的综合处理、组合利用、营养补充和草食畜各饲料源的互作效应,以调节动体“消化环境”,使秸秆的利用还存在着一定的缺憾。 一、新型秸秆生化剂的创新 新型秸秆生化培养剂产品的创新点是:①通过处理使粗纤维降低了11~16个百分点;②通过一定物质的添加调整。 展开更多
关键词 稻草发酵 经济效益 粗饲料 秸秆 生化培养剂
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免疫治疗中含血清及无血清培养剂对人周围血单个核细胞的影响
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作者 严佩华 《上海医科大学学报》 CSCD 1995年第6期433-437,共5页
以不同浓度抗CD_3的单克隆抗体(OKT_8),固相结合于培养瓶,激活正常人外周血单个核细胞(PBMC),于含血清培养剂和无血清培养剂(X-ViVo10)。体外实验中以OKT_8激活后细胞表型表达变化,发现T细胞、回忆细胞(CD_(45)Ro^+)数量明显增加,自然... 以不同浓度抗CD_3的单克隆抗体(OKT_8),固相结合于培养瓶,激活正常人外周血单个核细胞(PBMC),于含血清培养剂和无血清培养剂(X-ViVo10)。体外实验中以OKT_8激活后细胞表型表达变化,发现T细胞、回忆细胞(CD_(45)Ro^+)数量明显增加,自然杀伤细胞(NK)和B细胞减少,IL-Ⅱr^+(CD_(25))细胞,激活细胞(Tal^+)表达增加,甚至发生于刺激后第一天。但随OKT_3浓度增加而有细胞存活率降低现象。实验结果表明X-ViVo10无血清培养剂与有血清培养剂有同样效果,且可避免致敏、污染等副作用,故可用于免疫治疗。 展开更多
关键词 肿瘤 免疫疗法 自然杀伤细胞 血清 培养剂
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秸秆生化培养剂
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作者 刘禹宏 《农业科技与信息》 1998年第9期27-27,共1页
在我国大力推广秸秆养牛,过腹还田的今天,农作物秸秆无可非议成了反刍家畜的重要粗饲料源,但要真正合理有效利用,却是一个十分复杂的科学问题。不能简单地推崇“秸秆+尿素或微生物+秸秆=牛肉”的恒等式,纵观我国近几年的秸秆养牛实践,... 在我国大力推广秸秆养牛,过腹还田的今天,农作物秸秆无可非议成了反刍家畜的重要粗饲料源,但要真正合理有效利用,却是一个十分复杂的科学问题。不能简单地推崇“秸秆+尿素或微生物+秸秆=牛肉”的恒等式,纵观我国近几年的秸秆养牛实践,虽然取得一定效果,但几乎都集中在物理、化学、生物的处理方法上,而忽视了秸秆饲料的综合处理。 展开更多
关键词 秸秆 生化饲料 培养剂
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人工生物饵料培养剂:50DE微囊
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《畜牧市场》 2003年第3期21-21,共1页
关键词 人工生物饵料培养剂 50DE微囊 水产养殖 产品特点
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成骨细胞与诱导剂对骨髓基质干细胞的增殖与成骨分化的影响 被引量:10
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作者 黄开 章庆国 蔡国锋 《中国修复重建外科杂志》 CAS CSCD 北大核心 2006年第2期125-129,共5页
目的探索一种新的骨髓基质干细胞(marrowstromalstemcells,MSCs)增殖与成骨分化的体外诱导培养体系。方法乳大鼠颅骨来源的第3代成骨细胞与诱导剂(1nmol/L地塞米松、10mmol/Lβ-甘油磷酸钠、50μg/ml抗坏血酸)对大鼠股骨、胫骨来源的MSC... 目的探索一种新的骨髓基质干细胞(marrowstromalstemcells,MSCs)增殖与成骨分化的体外诱导培养体系。方法乳大鼠颅骨来源的第3代成骨细胞与诱导剂(1nmol/L地塞米松、10mmol/Lβ-甘油磷酸钠、50μg/ml抗坏血酸)对大鼠股骨、胫骨来源的MSCs生长的影响。于8块24孔板上培养MSCs,每孔接种5×104个第3代MSCs。按培养成分不同分为4组,每组2块。DMEM培养为对照组;诱导剂培养为诱导剂组;成骨细胞培养为成骨细胞组;联合使用成骨细胞与诱导剂培养为联合诱导组。计数诱导1~8d各组MSCs的数量并绘制细胞生长曲线,检测诱导10d的MSCs的碱性磷酸酶活性,采用RT-PCR检测诱导2周时MSCs骨钙素mRNA的表达水平。结果原代及传代MSCs形态正常。细胞生长曲线示MSCs数量均随时间延长增加。成骨细胞组增殖最快,诱导剂组增殖最慢,5~8d成骨细胞组及诱导剂组细胞数量与对照组比较,差异有统计学意义(P<0.05)。联合诱导组碱性磷酸酶活性为2.01±0.56U与对照组0.68±0.14U、诱导剂组1.27±0.43U及成骨细胞组0.77±0.19U比较,差异均有统计学意义(P<0.05)。对照组不表达骨钙素mRNA,诱导剂组为0.783±0.094、联合诱导组为0.814±0.071与成骨细胞组0.302±0.026比较,差异均有统计学意义(P<0.05)。结论联合使用成骨细胞和诱导剂诱导MSCs,不影响MSCs的正常增殖而促进MSCs的成骨分化,诱导效果较好,可望成为一种新的骨组织工程种子细胞的体外培养体系。 展开更多
关键词 骨组织工程 骨髓基质干细胞 成骨细胞 诱导培养
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发酵稻草饲喂肥育肉牛消化率及消化能的研究 被引量:4
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作者 宋金昌 刘禹宏 +1 位作者 范莉 韩淑珍 《河北职业技术师范学院学报》 2000年第2期21-23,共3页
采用一种生物化学及生物活性物复合制剂 ,无需外加微生物 ,直接发酵搓揉处理的秸秆 ,靠天然有益菌的快速的增殖 ,并抑制杂菌的生长 ,降解秸秆粗纤维 ,增加菌体蛋白质 ,提高秸秆的生物学价值和有效养分的利用。用该生化培养剂处理稻草 ,... 采用一种生物化学及生物活性物复合制剂 ,无需外加微生物 ,直接发酵搓揉处理的秸秆 ,靠天然有益菌的快速的增殖 ,并抑制杂菌的生长 ,降解秸秆粗纤维 ,增加菌体蛋白质 ,提高秸秆的生物学价值和有效养分的利用。用该生化培养剂处理稻草 ,蛋白质含量可提高 2 6 0~ 3 39个百分点 ,可降低粗纤维 1 0 4 2~ 1 6 5 9个百分点。以秸秆生化培养剂发酵稻草 70 86 %、发酵脱毒棉籽饼 1 2 2 7%、大豆粕 1 5 34 %、食盐 1 5 %组成的饲粮 ,饲喂舍饲杂交肥育肉牛 ,日增重达到 1 5 0 0~ 1 833g ,增重料比达 3 5 6~ 4 35。 展开更多
关键词 饲料 稻草发酵 生化培养剂 消化 消化能 肉牛
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白腐菌在秸秆堆肥化中的应用 被引量:8
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作者 王连稹 王祯丽 黄华波 《石河子大学学报(自然科学版)》 CAS 2003年第2期161-164,共4页
提出了处理农作物秸秆的堆肥化方法,分析了在堆肥中加入微生物白腐菌的可行性,并对白腐菌的适宜培养条件和堆肥的堆腐条件进行了阐述。
关键词 农作物秸秆 堆肥化 白腐菌 培养条件 堆腐条件 微生物发酵 微生物培养剂
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Influences of Carbon Sources and Plant Growth Regulators on Anther Culture Efficiency of Pepper 被引量:9
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作者 赵激 邹学校 +2 位作者 张竹青 杨博智 周书栋 《Agricultural Science & Technology》 CAS 2010年第4期102-105,共4页
[Objective]In order to increase anther culture efficiency of pepper.[Method]MS culture media and Bolajiaohong were used in this experiment to study the influences of carbon sources and concentrations on anther callus ... [Objective]In order to increase anther culture efficiency of pepper.[Method]MS culture media and Bolajiaohong were used in this experiment to study the influences of carbon sources and concentrations on anther callus induction of pepper.Jiayu was taken as a material to study influences of plant growth regulators and concentrations on anther callus induction of pepper according to L16(4^5) orthogonal design.[Result]The average callus and embryoid induction rates of maltose at all concentrations were higher than these of sucrose but the difference was not significant.Taking maltose or sucrose as a carbon source,3% to 6% concentration was good for increasing induction frequencies of calli and embryoids.However,If the concentration was over 6%,the induction rates were declined dramatically with the increase of sugar concentration.The influences of growth regulators on induction rate of calli were listed as 2,4-D﹥ZT﹥NAA﹥KT﹥6-BA;the influences on induction rates of embryoids were listed as 2,4-D﹥NAA﹥ZT﹥KT﹥6-BA.The 2,4-D,ZT,NAA and KT had signficant or extremely significant influences on induction rates of calli and embryoids.2,4-D,ZT at 1.0 mg/L and NNA,KT at 0.5 mg/L had the best effects.The influences of ZT on calli and embryoids were better than those of KT and 6-BA.1.0 mg/L 2,4-D +1.0 mg/L ZT +0.5 mg/L KT +0.5 mg/L 6-BA was the best regulator combination for induction culture of Jiayu anther.[Conclusion]The experiment provided research basis for anther culture of pepper. 展开更多
关键词 PEPPER Anther culture Carbon source Plant growth regulator CALLUS EMBRYOID
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Yeast Culture and Its Application in Feed 被引量:3
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作者 徐丽萍 《Agricultural Science & Technology》 CAS 2014年第12期2183-2186,共4页
Yeast culture is a kind of green feed additive. It has been researched and applied widely in the feed industry. In this paper, the functions of yeast culture and its application in the actual production were discussed... Yeast culture is a kind of green feed additive. It has been researched and applied widely in the feed industry. In this paper, the functions of yeast culture and its application in the actual production were discussed. It can be concluded that yeast culture has a great application prospect. 展开更多
关键词 Yeast culture Feed additive Animal feeding BENEFIT
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Progress of safflower (Carthamus tinctorius L.) regeneration through tissue culture 被引量:9
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作者 FAN Lijiao GUO Meili 《Journal of Medical Colleges of PLA(China)》 CAS 2013年第5期289-301,共13页
Background: Safflower regeneration through tissue culture has long been limited to low frequency and lack of an efficient protocol that suitable for most safflower cultivars. In past decades, researches had been carri... Background: Safflower regeneration through tissue culture has long been limited to low frequency and lack of an efficient protocol that suitable for most safflower cultivars. In past decades, researches had been carried out to investigate safflower regeneration through tissue culture and great progress had been made. Objective: To investigate factors that affect safflower regeneration through tissue culture principally. Methods: This article summarized available literatures about advancements in safflower regeneration, especially discussed factors affecting safflower tissue culture in detail. Results: Safflower regeneration was fairly hard than other congeneric plants, such as chrysanthemum. The genotype, seedling age, type of explants, medium components, plant growth regulators and other additives all had specific influences on safflower tissue culture. More deepgoing researches need to be undertaken to establish an effective safflower regeneration system. 展开更多
关键词 SAFFLOWER Carthamus tinctorius L. REGENERATION Tissue c .ulture
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Microbial profile and antibiotic sensitivity pattern in bile cultures from endoscopic retrograde cholangiography patients 被引量:13
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作者 Muhsin Kaya Remzi Bestas +3 位作者 Fatma Bacalan Ferhat Bacaksz Esma Gülsun Arslan Mehmet Ali Kaplan 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第27期3585-3589,共5页
AIM: To identify the frequency of bacterial growth, the most commonly grown bacteria and their antibiotic susceptibility, and risk factors for bacterial colonization in bile collected from patients with different bil... AIM: To identify the frequency of bacterial growth, the most commonly grown bacteria and their antibiotic susceptibility, and risk factors for bacterial colonization in bile collected from patients with different biliary dis- eases. METHODS: This prospective study was conducted be- tween April 2010 and August 2011. Patients with vari- ous biliary disorders were included. Bile was aspirated by placing a single-use, 5F, standard sphincterotome catheter into the bile duct before the injection of con- trast agent during endoscopic retrograde cholangio- pancreaticography (ERCP). Bile specimens were trans- ported to the microbiology laboratory in blood culture bottles within an anaerobic transport system. Bacteria were cultured and identified according to the standard protocol used in our clinical microbiology laboratory. The susceptibilities of the organisms recovered were identified using antimicrobial disks, chosen according tothe initial gram stain of the positive cultures RESULTS: Ninety-one patients (27% male, mean age 53.7 4- 17.5 years, range: 17-86 years) were included in the study. The main indication for ERCP was benign biliary disease in 79 patients and malignant disease in 12 patients. The bile culture was positive for bacterial growth in 46 out of 91 (50.5%) patients. The most frequently encountered organisms were Gram-negative bacteria including Escherich/a coli (28.2%), Pseudo- monas (17.3%) and Stenotrophomonas rnaltophilia (15.2%). There were no significant differences between patients with malignant and benign disease (58% vs 49%, P = 0.474), patients with acute cholangitis and without acute cholangitis (52.9% vs 50%, P = 0.827), patients who were empirically administered antibiotics before intervention and not administered (51.4% vs 60.7%, P = 0.384), with regard to the bacteriobilia. We observed a large covering spectrum or low resistance to meropenem, amikacin and imipenem. CONCLUSION: We did not find a significant risk fac- tor for bacteriobilia in patients with biliary obstruction. A bile sample for microbiological analysis may become a valuable diagnostic tool as it leads to more accurate selection of antibiotics for the treatment of cholangitis. 展开更多
关键词 CHOLANGITIS Endoscopic retrograde cholan-giopancreaticography Bacteriobilia Bile culture
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洋虫的生物学特性及饲养
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作者 李德泽 《中草药》 CAS 1984年第8期25-26,共2页
洋虫是一种家养昆虫药。本文重点介绍了洋虫成虫的形态与习性,对临床试用情况及饲养方法也作了简要介绍。附洋虫成虫、蛹及幼虫图。
关键词 洋虫 拟步行虫科 培养填充 红花 保暖
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Callus and Adventitious Buds Formation from Stem Explants of Balsam Pear (Momordica charantia L.) 被引量:2
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作者 Y. Tang L. Wang C. Ma B. Liu J. Liu H. X. Li 《Journal of Agricultural Science and Technology(B)》 2011年第1期146-150,共5页
The effects of sterilization pretreatment, sampling time and growth regulators on callus formation, explant contamination and adventitious bud induction from stems of balsam pear (Momordica charantia L.) were invest... The effects of sterilization pretreatment, sampling time and growth regulators on callus formation, explant contamination and adventitious bud induction from stems of balsam pear (Momordica charantia L.) were investigated. Sterilized for 6 rain with 0.1% (w/v) mercuric chloride solution is an essential sterilization method for stems. June and July proved to be better for material taking, followed by May. The callus formation rate of balsam pears reached the highest when medium added with IBA 1.0mg/L and BA 2.0 mg/L, while the optimum medium for adventitious bud induction was MS added with IBA 0.5mg/L and BA 4.0 mg/L. And it will provide theoretical and technical basis for rapid propagation and breeding. 展开更多
关键词 Balsam pear (Momordica charantia L.) tissue culture adventitious buds
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Preparation of Dried Culture of Probiotic Rhizopus oryzae in Two Different Mediums as Feed Additive to Indigenous Chicken
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作者 Turrini Yudiarti Vitus Dwi Yunianto Budi Ismadi Isroli 《Journal of Agricultural Science and Technology(A)》 2014年第10期874-879,共6页
Fungus Rhizopus oryzae has a probiotic potential, but the potency is not yet explored. Probiotics are usually used as feed additive, including to the chicken. Probiotic needs a suitable medium for their life. The aim ... Fungus Rhizopus oryzae has a probiotic potential, but the potency is not yet explored. Probiotics are usually used as feed additive, including to the chicken. Probiotic needs a suitable medium for their life. The aim of the study was to know which preparations can produce suitable medium for the growth of Rhizopus oryzae on the dried culture formed. The study used two preparations. Preparation 1 (a) consisted of rice bran (1 kg) and culture ofRhizopus oryzae on Potato Dextrose Broth (PDB medium) (500 mL); then preparation 1 (b) consisted of corn grain (1 kg) and culture of Rhizopus oryzae on PDB medium (500 mL). Preparation 2 (a) consisted of rice bran (1 kg), culture of Rhizopus oryzae on Potato Dextrose Agar (PDA medium) (seven petri dishes) and was added with sterile distilled water (500 mL); preparation 2 (b) consisted of corn grain (1 kg), culture of Rhizopus oryzae on PDA medium (seven petri dishes) and was added with sterile distilled water (500 mL). The culture of Rhizopus oryzae even both on PDB and PDA were 3 d of incubation. All preparations were incubated for 7 d then they were dried under the sun. Parameter evaluation was the growth performance of the fungus in the dried culture media. The growth performance was analyzed by microscopic analysis and the number of colony of the fungi in the two dried culture media. The results showed that from the macroscopic analysis, the sample of both dried culture media which were placed on PDA medium showed the growth of mycelia and spores of the same kind of fungus and it was proved by microscopic observation that the fruiting bodies (mycelia and spores) of that ftmgus was Rhizopus oryzae. The colonies number of the fungi from preparation 1 (a) and 1 (b) were 0,4 × 10 cfu/gr and 2.4 × 10^3 cfu/gr, respectively, and from preparation 2 (a) and 2 (b) were 1.3 × 10^5 cfu/gr and 8.3 ×10^2 cfu/gr, respectively. It could be concluded that the product of the dried culture of probiotic Rizopus oryzae from the preparation 2 with rice brand or corn grain mediums can be used as feed additive to indigenous chicken. 展开更多
关键词 Feed additive probiotic dried culture chicken.
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Enhancement of the Photodynamic Therapy Effect on Streptococcus Mutans Biofilm 被引量:1
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作者 Vitor Hugo Panhoca Mariana Carreira Geralde +3 位作者 Thaila Quatrini Correa Mariana Torres Carvalho Clovis Wesley Oliveira Souza Vanderlei Salvador Bagnato 《Journal of Physical Science and Application》 2014年第2期107-114,共8页
Biofilm is a community of bacteria, less susceptible to traditions treatments. Although photodynamic therapy (PDT) is a very effective way to microorganism inactivation, in biofilm it is not as efficient as it is in... Biofilm is a community of bacteria, less susceptible to traditions treatments. Although photodynamic therapy (PDT) is a very effective way to microorganism inactivation, in biofilm it is not as efficient as it is in planktonic bacteria cultures. The increment of an element to increase the effectiveness of PDT was our aim. Therefore, this in vitro study evaluates the susceptibility ofa biofilm formed by Streptococcus mutans on metallic surface of orthodontic accessories under the application of PDT with a surfactant. Samples obtained from blades of orthodontic bands (NiCr), where used as adhesion surface for the biofilm. They were treated with 1 mg/ml of curcumin, with 0.1% of sodium dodecyl sulfate and exposed to 30 J/cm^2 of light (455 nm). Eight experimental groups were studied, including the positive and negative controls. The results show that the group with PDT and surfactant had a significant decrease (p 〈 0.001) in viability. In this case, the reduction observed was of 5.6 log10 (CFU/ml) in comparison to the control group. We have shown that, even though the biofilm is very tough and complex structure, we are able to promote almost the complete inactivation ofS. mutans in systems similar to an orthodontic treated patient's mouth. 展开更多
关键词 Photodynamic therapy light-induced damage oral biofilm streptococcus mutans CURCUMIN SURFACTANT
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Davana: A New Host Plant for Ralstonia solanacearum from India
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作者 M. K. Prasannakumar K. N. Chandrashekara +1 位作者 M. Deepa A. Vani 《Journal of Agricultural Science and Technology(A)》 2011年第1X期81-88,共8页
Ralstonia solanacearum infecting Davana (Artemisia pallens Wall.) from commercial nurseries in India was isolated on modified semi selective media (SMSA). Here, we report a new host for Ralstonia solanacearum i.e.... Ralstonia solanacearum infecting Davana (Artemisia pallens Wall.) from commercial nurseries in India was isolated on modified semi selective media (SMSA). Here, we report a new host for Ralstonia solanacearum i.e. davana. It has huge demand in medicinal and aromatic industries. Isolate was confirmed as race-l, biovar-3 by morphological, physiological, biochemical and pathogenicity studies. Two sets of primers (OLI 1 & Y2 and Y 1 & Y2) were used in this study. Further, the identity of the isolate was confirmed by serological diagnostic kit obtained from International Potato Research Center, Lima, Peru and single chain variable fragment antibody specific to Ralstonia solanacearum used to confirm the casual organism. 展开更多
关键词 Davana new host Ralstonia solanacearum serological diagnostic kit single chain variable fragment antibody
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