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辅助生殖培养用液中庆大霉素临床前安全评价思考
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作者 管瑞芹 王召旭 +1 位作者 姜爱莉 柯林楠 《中国药事》 CAS 2022年第12期1377-1382,共6页
目的:归纳总结庆大霉素对配子和胚胎的影响,并对辅助生殖培养用液临床前评价需要考虑的问题进行分析,为辅助生殖培养用液产品中庆大霉素的临床前安全评价提供参考。方法:以辅助生殖培养用液中添加的药物庆大霉素为研究对象,结合庆大霉... 目的:归纳总结庆大霉素对配子和胚胎的影响,并对辅助生殖培养用液临床前评价需要考虑的问题进行分析,为辅助生殖培养用液产品中庆大霉素的临床前安全评价提供参考。方法:以辅助生殖培养用液中添加的药物庆大霉素为研究对象,结合庆大霉素在产品中的应用,采用文献调研法总结归纳了庆大霉素对生殖细胞、胚胎以及胚胎干细胞产生的影响,并根据辅助生殖用液产品监管现状,提出临床前安全评价思考。结果与结论:庆大霉素被广泛应用在辅助生殖培养用液中,安全、规范地使用庆大霉素在辅助生殖技术中具有重要意义。目前有关庆大霉素对配子及胚胎毒性的研究较少,已知的结论都是基于鼠胚试验及其他动物试验得出的结果扩展到人类胚胎,有必要关注庆大霉素对人类配子及胚胎发育的影响。其次,对于辅助生殖用液产品中庆大霉素添加量的确定、原料质量控制以及检测方法等方面存在的问题提出有针对性的建议,为加强辅助生殖用液的质量控制和产业监管提供思路,从而促进辅助生殖技术的发展。 展开更多
关键词 人类辅助生殖技术 培养用液 庆大霉素 配子 胚胎 临床前评价 质量监管
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肾小管细胞培养技术研究进展
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作者 黄珀 邓勇 +1 位作者 郭勇 冯志强 《泸州医学院学报》 2001年第3期266-267,共2页
关键词 肾小管细胞 细胞培养 取材 培养用液 分离培养
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ALA Altered ABA Content of Winter Oilseed Rape(Brassica napus L.)Seedling 被引量:1
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作者 张树杰 李玲 +1 位作者 张春雷 李光明 《Agricultural Science & Technology》 CAS 2011年第4期484-488,591,共6页
[Objective] The paper was to explore the impact of 5-aminolevunic acid(ALA)treatment on growth of winter oilseed rape(Brassica napus L.)seedlings and its mechanism.[Method] The effects of ALA on abscisic acid(ABA... [Objective] The paper was to explore the impact of 5-aminolevunic acid(ALA)treatment on growth of winter oilseed rape(Brassica napus L.)seedlings and its mechanism.[Method] The effects of ALA on abscisic acid(ABA)contents in plant organs and xylem saps were investigated through hydroponic experiment.Four treatments including seed soaking with 0,25,50 and 75 mg/L ALA solutions(CK,A1,A2 and A3)for 6 h were set in the test.[Result] In addition to A3 treatment which made the dry weights of oilseed rape seedlings slightly decrease,after seed soaking with ALA,dry weights,net photosynthetic rate(Pn),transpiration rate(Tr)and stomatal conductance(Gs)of plants significantly increased compared with control.ABA concentrations in plant shoots and xylem saps increased in different levels after seed soaking with ALA.[Conclusion] The increasing endogenous ABA contents might be an explanation for promotion effect of ALA application on the growth of winter oilseed rape seedling. 展开更多
关键词 Oilseed rape Hydroponic culture Xylem sap PHOTOSYNTHESIS PHYTOHORMONE
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Response of Lettuce to the Coupling Electrical Conductivity and Circulation Rate of the Nutrient Solution in Hydroponics 被引量:2
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作者 LIANG Hao QI Jing-Wei +2 位作者 JI Yan-Hai LIU Ming-Chi WU Zhan-Hui 《Journal of Life Sciences》 2017年第4期202-205,共4页
The coupling effect of nutrient solution EC (electrical conductivity) levels and CR (circulation rate) on the morphology and quality, in hydroponically grown lettuce was assessed. Lettuce was grown at 5 treatments... The coupling effect of nutrient solution EC (electrical conductivity) levels and CR (circulation rate) on the morphology and quality, in hydroponically grown lettuce was assessed. Lettuce was grown at 5 treatments fi'om High EC & Low CR to Low EC & High CR. The environmental parameters were controlled in a 20 m^2 plant factory during the hydroponic cultivation with the following values: irradiated by blue and red light-emitting diode lighting with PPFD (photosynthetic photon flux density) value of 150 mol·m^-2·s^-1 for 16 hours per day; Temperature was maintained at 22.0 ℃ during the photoperiod and 16.0 ℃ in dark cycle. The results demonstrate that growing lettuce can be adopted using nutrient solution with lower EC levels and higher CR. The results also indicate that the effect of bi-directional coupled EC and CR resulted in expansion of root length but reducing the root biomass. Nitrate content was significantly reduced. 展开更多
关键词 LED lighting LETTUCE HYDROPONICS flow rate of nutrient solution nutrition supply.
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