The stages of introduction in vitro culture of the local population of the kidney tea （Orthosiphon stamineus Bents） cultivated in the Georgia’s medicinal plant farm and the microclonal propagation, in particular, h...The stages of introduction in vitro culture of the local population of the kidney tea （Orthosiphon stamineus Bents） cultivated in the Georgia’s medicinal plant farm and the microclonal propagation, in particular, have been elaborated. The cultivation of explants was carried out on the Gamborg （B5） feeding area. The hormonal （BAP; Zn; NAA） composition of the feeding area and their concentrations have been selected; proliferation of buds in the basal part of the sprout has been achieved from the formed morphogenic tissue. The microclones by activating axillary meristem have been received.展开更多
In vitro callus induction and plant regeneration potentiality were studied from mature embryo of three Indian rice （Oryza sativa L.） groups at Field Crops Department, Agricultural Faculty, Ondokuz May？s University,...In vitro callus induction and plant regeneration potentiality were studied from mature embryo of three Indian rice （Oryza sativa L.） groups at Field Crops Department, Agricultural Faculty, Ondokuz May？s University, Samsun, Turkey. The study was done by using callus induction MS medium having different concentration of four. The present research was conducted according to the design of randomized blocks trial. A total of 696 calluses, 193 plants and 917 seeds were obtained from Indica group; 2,110 calluses, 103 plants and 235 seeds were obtained from Japonica group; 1,243 calluses and 13 plants were obtained from Javanica group. With regard to number of calluses obtained from each explant source, 52 calluses were obtained from whole-plant explants, 1,668 calluses from root explants, 629 calluses from shoot explants, 649 calluses from the 1st node explants, 240 calluses from the 2nd node explants, 269 calluses from the 1st internode explants and 12 calluses from the 2nd internode explants. With regard to number of plants obtained from each explant source, 27 plants were obtained from whole-plant explants, 195 plants from shoot explants, 43 plants from the 1st node explants, 40 plants from the 2nd node explants and four plants from the 1st internode explants. With regard to number of seeds, 823 seeds were obtained from shoot explants and 329 seeds were obtained from the 2nd node explants. Germination rate of harvested seeds was over 90%. The establishment of this regeneration system is essential for the development of a genetic transformation system for commercial rice cultivars.展开更多
This study aims at determining and comparing the in vitro antifungal activity of six EOs （essential oils） extracted from Monodora myristica, Melaleuca quinquenervia, Eucalyptus torelliana, Chenopodium ambrosioides, ...This study aims at determining and comparing the in vitro antifungal activity of six EOs （essential oils） extracted from Monodora myristica, Melaleuca quinquenervia, Eucalyptus torelliana, Chenopodium ambrosioides, fresh Zingiber officinalis and dehydrated Zingiber officinalis with those of three synthetic fungicides （Callicuivre, Banko plus and Mancozeb） on Sclerotium rolfsii a pathogenic fungus of tomato in Cote d＇Ivoire. The products were added to PDA （potato dextrose agar） medium at various concentrations. The results showed different aspects of the mycelium, different activities on mycelium growth and sclerotia production depending on the explants （mycelium or sclerotia）. Depending on their IC and MGI （mycelium growth inhibition）, the oils stemming from Chenopodium ambrosioides and fresh Zingiber officinalis showed more inhibitory activities on mycelial growth （reduction rate of 100%） and sclerotia production at 250 ppm. These activities were close to or even greater than those of fungicides. The mancozeb fungicide showed stronger activity than the others by inhibiting mycelium growth and sclerotia production of mycelium explants at 250 ppm. This study offers the possibility of using EOs in the control strategies of Sclerotium rolfsii.展开更多
文摘The stages of introduction in vitro culture of the local population of the kidney tea （Orthosiphon stamineus Bents） cultivated in the Georgia’s medicinal plant farm and the microclonal propagation, in particular, have been elaborated. The cultivation of explants was carried out on the Gamborg （B5） feeding area. The hormonal （BAP; Zn; NAA） composition of the feeding area and their concentrations have been selected; proliferation of buds in the basal part of the sprout has been achieved from the formed morphogenic tissue. The microclones by activating axillary meristem have been received.
文摘In vitro callus induction and plant regeneration potentiality were studied from mature embryo of three Indian rice （Oryza sativa L.） groups at Field Crops Department, Agricultural Faculty, Ondokuz May？s University, Samsun, Turkey. The study was done by using callus induction MS medium having different concentration of four. The present research was conducted according to the design of randomized blocks trial. A total of 696 calluses, 193 plants and 917 seeds were obtained from Indica group; 2,110 calluses, 103 plants and 235 seeds were obtained from Japonica group; 1,243 calluses and 13 plants were obtained from Javanica group. With regard to number of calluses obtained from each explant source, 52 calluses were obtained from whole-plant explants, 1,668 calluses from root explants, 629 calluses from shoot explants, 649 calluses from the 1st node explants, 240 calluses from the 2nd node explants, 269 calluses from the 1st internode explants and 12 calluses from the 2nd internode explants. With regard to number of plants obtained from each explant source, 27 plants were obtained from whole-plant explants, 195 plants from shoot explants, 43 plants from the 1st node explants, 40 plants from the 2nd node explants and four plants from the 1st internode explants. With regard to number of seeds, 823 seeds were obtained from shoot explants and 329 seeds were obtained from the 2nd node explants. Germination rate of harvested seeds was over 90%. The establishment of this regeneration system is essential for the development of a genetic transformation system for commercial rice cultivars.
文摘This study aims at determining and comparing the in vitro antifungal activity of six EOs （essential oils） extracted from Monodora myristica, Melaleuca quinquenervia, Eucalyptus torelliana, Chenopodium ambrosioides, fresh Zingiber officinalis and dehydrated Zingiber officinalis with those of three synthetic fungicides （Callicuivre, Banko plus and Mancozeb） on Sclerotium rolfsii a pathogenic fungus of tomato in Cote d＇Ivoire. The products were added to PDA （potato dextrose agar） medium at various concentrations. The results showed different aspects of the mycelium, different activities on mycelium growth and sclerotia production depending on the explants （mycelium or sclerotia）. Depending on their IC and MGI （mycelium growth inhibition）, the oils stemming from Chenopodium ambrosioides and fresh Zingiber officinalis showed more inhibitory activities on mycelial growth （reduction rate of 100%） and sclerotia production at 250 ppm. These activities were close to or even greater than those of fungicides. The mancozeb fungicide showed stronger activity than the others by inhibiting mycelium growth and sclerotia production of mycelium explants at 250 ppm. This study offers the possibility of using EOs in the control strategies of Sclerotium rolfsii.
