Cytological,biochemical and physiological charcteristics of quiescent,high-vigor carrot somatic embryos obtained with the use of regulation culture were studied. As shown in cytological observation, quiescent somatic ...Cytological,biochemical and physiological charcteristics of quiescent,high-vigor carrot somatic embryos obtained with the use of regulation culture were studied. As shown in cytological observation, quiescent somatic embryos obtained by regulation culturing for 70 days displayed small compacted cells with thick wall, dense cytoplasm and large amount of starch granules. Biochemical tests revealed a starch content and a soluble sugar content 1.9 and 1.7 times respectively as much as those of the control embryos(normal culture) while physiological study demonstrated that the respiration was maintained stally at a very low level,only 1/4 that of the control at the end of the 70-day period. These results provide further evidences that regution culture so after the morphology, biochemistcy, physiologr and metabolism of somatic embryos that they became quiescent,highly synchronized, and had their roots ungrown, a sate facilitating safe storage.展开更多
Somatic embryo is widely used in genetic engineering and cell engineering. This paper reviewed the recent research results of somatic embryogenesis in Cucurbitaceae plants. Somatic embryogenesis is controlled by many ...Somatic embryo is widely used in genetic engineering and cell engineering. This paper reviewed the recent research results of somatic embryogenesis in Cucurbitaceae plants. Somatic embryogenesis is controlled by many factors such as genotype, explant type, seedling age, basal culture medium, carbohydrate, nitrogen, growth regulators, additives and illumination et al.. Abnormality, desynchrony and browning are the main problem existing in Cucurbitaceae somatic embryogenesis. Then some ideas on how to obtain high quality somatic embryo are given. At last, we forecast the application of somatic embryo in breeding of Cucurbitaceae plants.展开更多
文摘Cytological,biochemical and physiological charcteristics of quiescent,high-vigor carrot somatic embryos obtained with the use of regulation culture were studied. As shown in cytological observation, quiescent somatic embryos obtained by regulation culturing for 70 days displayed small compacted cells with thick wall, dense cytoplasm and large amount of starch granules. Biochemical tests revealed a starch content and a soluble sugar content 1.9 and 1.7 times respectively as much as those of the control embryos(normal culture) while physiological study demonstrated that the respiration was maintained stally at a very low level,only 1/4 that of the control at the end of the 70-day period. These results provide further evidences that regution culture so after the morphology, biochemistcy, physiologr and metabolism of somatic embryos that they became quiescent,highly synchronized, and had their roots ungrown, a sate facilitating safe storage.
文摘Somatic embryo is widely used in genetic engineering and cell engineering. This paper reviewed the recent research results of somatic embryogenesis in Cucurbitaceae plants. Somatic embryogenesis is controlled by many factors such as genotype, explant type, seedling age, basal culture medium, carbohydrate, nitrogen, growth regulators, additives and illumination et al.. Abnormality, desynchrony and browning are the main problem existing in Cucurbitaceae somatic embryogenesis. Then some ideas on how to obtain high quality somatic embryo are given. At last, we forecast the application of somatic embryo in breeding of Cucurbitaceae plants.
