The sectioned thin cell layers (TCL) of flower stalk of Cichorium intybus L. were cultured in MS medium supplemented with NAA and BA or IAA and BA where floral and vegetative buds were developed from the explant. ...The sectioned thin cell layers (TCL) of flower stalk of Cichorium intybus L. were cultured in MS medium supplemented with NAA and BA or IAA and BA where floral and vegetative buds were developed from the explant. Endogenous IAA, DHZ+DHZR, iPA increased significantly during the floral bud formation, while Z+ZR remained changed. The levels of cytokinins, DHZ+DHZR, iPA, and Z+ZR all increased significantly during the vegetative bud formation, however IAA level was reduced during the first 7 days of culture and increased to two thirds of initial values on the day when the bud primordia were formed. The results suggested that the initiation of floral buds was associated with a high IAA/CTK ratio, whereas the induction of vegetative bud differentiation was related to a low IAA/CTK ratio.展开更多
bacterium LV-1 which isolated from soil sample were studied bacterium was isolated by serial dilution method, the effects of carbon source, nitrogen source, the initial pH and temperature on producing polysaccharide b...bacterium LV-1 which isolated from soil sample were studied bacterium was isolated by serial dilution method, the effects of carbon source, nitrogen source, the initial pH and temperature on producing polysaccharide by it were discussed to confirm the optimum fermentation conditions. [ Result] The physicochemical properties showed that the polysaccharide was water-soluble, but insoluble in organic solvents including ethanol, butanol, and chloroform. It was neutral polysaccharide with negative charge and without reducing terminal. The pH of its solution was pH =7.5. There were no protein, fructose, uronic acid, sulphate and starch-like structure included in po/ysacchadde molecules. The optimum fermentation conditions for po/ysaccharide produc- tion were 3% mannitol as carbon source, 0.25% yeast extract as nitrogen source, culture temperature 28 ~（3 and pH =7.5. [Coadu^en] The re- search could provide basis for development and utilization of LV-1 and industrialized production of mucopolysaccharide.展开更多
[Objective] The aim was to investigate differences in differentiation and regeneration of the explants from different parts of Lilium lancifolium（Yixing Lily） in tissue culture.[Method] The different parts of scale,...[Objective] The aim was to investigate differences in differentiation and regeneration of the explants from different parts of Lilium lancifolium（Yixing Lily） in tissue culture.[Method] The different parts of scale,leaf and root of Yixing Lily were cultured as explants on MS basic medium supplemented with different concentrations of plant growth regulators,so as to compare their capacity to differentiate and regenerate.[Result] The explants had different potential to differentiate（scale root leaf）.The capacity of different scale parts to differentiate was the lower part middle partupper part;the capacity of different leaf parts to differentiate was the leaf base middle part leaf tip;the capacity of different root parts to differentiate was the root base root tip middle part.[Conclusion] Tissue culture could be well applied in propagation of Yixing Lily.展开更多
Using seed sowing cultivation method, the four-season soilless cultivation of the sprouting seedlings of Toona sinensis was carried out with different germination accelerating treatments, cultivation substrates, culti...Using seed sowing cultivation method, the four-season soilless cultivation of the sprouting seedlings of Toona sinensis was carried out with different germination accelerating treatments, cultivation substrates, cultivation environment and management technologies by using the corresponding treatment methods in different seasons, so as to produce the sprouting vegetable products of T. sinensis which was not restricted by seasons. The test results showed that using the mixed substrate of pedite and vermiculite in winter and spring can keep moisture but not too high, and the produced sprout vegetable had good quality and high yield.展开更多
To solve the problems of microbial contamination and no rooting during the tissue culture of Huperzia serrata, wild seedlings of Huperzia serrata were cultured by indoor hydroponics or soil cultivation methods, then t...To solve the problems of microbial contamination and no rooting during the tissue culture of Huperzia serrata, wild seedlings of Huperzia serrata were cultured by indoor hydroponics or soil cultivation methods, then the stem tips were used in tissue culture. The above operation significantly reduced microbial contamination during tissue culture process. Different types and concentrations of hormones were added into the basic medium MS to screen the optimal formula for induced plantlet growth and rooting. It was found that 1/2MSCO was the best medium on which stem, leaves and roots of tissue culture plantlets of Huperzia serrata grew quickly. At the same time, sand planting was a convenient and effective approach for gemmae propagation of Huperzia serrata, the survival rate of seedlings germinated from gemmae was nearly 100%.展开更多
Effects of NaCI on seed germination, leaf gas exchange and seedling growth were investigated. The germination of P. tatarinowii seeds was delayed in the presence of NaCl, and was significantly inhibited when NaCl conc...Effects of NaCI on seed germination, leaf gas exchange and seedling growth were investigated. The germination of P. tatarinowii seeds was delayed in the presence of NaCl, and was significantly inhibited when NaCl concentration exceeded 17 mM （1.0 g·L^-1）. NaCl treatments induced reductions of transpiration rate, stomatal conductance and net photosynthetic rate and their reductions were significant with the increasing of NaCl concentration. However, intercellular CO2 concentration in seedling leaves under salt stress was significantly higher than that of controls. There was a significant reduction in survival, basal diameter increment and height increment witl increasing NaCl concentration 50 days after NaCl treatment, and a significant reduction in the dry weight increment of roots, stems, and leaves was also observed in the NaCl treatments. The deleterious effect on the biomass increment was more evident in the case of stems and leaves. Results from this study suggest that P. tatarinowii is sensitive to salinity stress and the salinity threshold for seedling growth might be less 34 mM （2.0 g· L^-1）.展开更多
文摘The sectioned thin cell layers (TCL) of flower stalk of Cichorium intybus L. were cultured in MS medium supplemented with NAA and BA or IAA and BA where floral and vegetative buds were developed from the explant. Endogenous IAA, DHZ+DHZR, iPA increased significantly during the floral bud formation, while Z+ZR remained changed. The levels of cytokinins, DHZ+DHZR, iPA, and Z+ZR all increased significantly during the vegetative bud formation, however IAA level was reduced during the first 7 days of culture and increased to two thirds of initial values on the day when the bud primordia were formed. The results suggested that the initiation of floral buds was associated with a high IAA/CTK ratio, whereas the induction of vegetative bud differentiation was related to a low IAA/CTK ratio.
文摘bacterium LV-1 which isolated from soil sample were studied bacterium was isolated by serial dilution method, the effects of carbon source, nitrogen source, the initial pH and temperature on producing polysaccharide by it were discussed to confirm the optimum fermentation conditions. [ Result] The physicochemical properties showed that the polysaccharide was water-soluble, but insoluble in organic solvents including ethanol, butanol, and chloroform. It was neutral polysaccharide with negative charge and without reducing terminal. The pH of its solution was pH =7.5. There were no protein, fructose, uronic acid, sulphate and starch-like structure included in po/ysacchadde molecules. The optimum fermentation conditions for po/ysaccharide produc- tion were 3% mannitol as carbon source, 0.25% yeast extract as nitrogen source, culture temperature 28 ~（3 and pH =7.5. [Coadu^en] The re- search could provide basis for development and utilization of LV-1 and industrialized production of mucopolysaccharide.
基金Supported by Fund for Scientific Research in Yangtze University(CDKF2283)Program of Engineering Research Center of Wetland Agriculture in the Middle Reaches of the Yangtze River of Ministry of Education~~
文摘[Objective] The aim was to investigate differences in differentiation and regeneration of the explants from different parts of Lilium lancifolium（Yixing Lily） in tissue culture.[Method] The different parts of scale,leaf and root of Yixing Lily were cultured as explants on MS basic medium supplemented with different concentrations of plant growth regulators,so as to compare their capacity to differentiate and regenerate.[Result] The explants had different potential to differentiate（scale root leaf）.The capacity of different scale parts to differentiate was the lower part middle partupper part;the capacity of different leaf parts to differentiate was the leaf base middle part leaf tip;the capacity of different root parts to differentiate was the root base root tip middle part.[Conclusion] Tissue culture could be well applied in propagation of Yixing Lily.
文摘Using seed sowing cultivation method, the four-season soilless cultivation of the sprouting seedlings of Toona sinensis was carried out with different germination accelerating treatments, cultivation substrates, cultivation environment and management technologies by using the corresponding treatment methods in different seasons, so as to produce the sprouting vegetable products of T. sinensis which was not restricted by seasons. The test results showed that using the mixed substrate of pedite and vermiculite in winter and spring can keep moisture but not too high, and the produced sprout vegetable had good quality and high yield.
文摘To solve the problems of microbial contamination and no rooting during the tissue culture of Huperzia serrata, wild seedlings of Huperzia serrata were cultured by indoor hydroponics or soil cultivation methods, then the stem tips were used in tissue culture. The above operation significantly reduced microbial contamination during tissue culture process. Different types and concentrations of hormones were added into the basic medium MS to screen the optimal formula for induced plantlet growth and rooting. It was found that 1/2MSCO was the best medium on which stem, leaves and roots of tissue culture plantlets of Huperzia serrata grew quickly. At the same time, sand planting was a convenient and effective approach for gemmae propagation of Huperzia serrata, the survival rate of seedlings germinated from gemmae was nearly 100%.
基金Foundation project： This paper is supported by the National Natural Science Foundation of China （the grant number No.39970608）.
文摘Effects of NaCI on seed germination, leaf gas exchange and seedling growth were investigated. The germination of P. tatarinowii seeds was delayed in the presence of NaCl, and was significantly inhibited when NaCl concentration exceeded 17 mM （1.0 g·L^-1）. NaCl treatments induced reductions of transpiration rate, stomatal conductance and net photosynthetic rate and their reductions were significant with the increasing of NaCl concentration. However, intercellular CO2 concentration in seedling leaves under salt stress was significantly higher than that of controls. There was a significant reduction in survival, basal diameter increment and height increment witl increasing NaCl concentration 50 days after NaCl treatment, and a significant reduction in the dry weight increment of roots, stems, and leaves was also observed in the NaCl treatments. The deleterious effect on the biomass increment was more evident in the case of stems and leaves. Results from this study suggest that P. tatarinowii is sensitive to salinity stress and the salinity threshold for seedling growth might be less 34 mM （2.0 g· L^-1）.
