以基因工程强化微藻三酰甘油(TAG)积累是实现微藻生物柴油应用化的途径之一。为进行有效的基因工程操作,研究分析了微藻代谢网络,以找出其中对TAG合成重要的反应。构建了异养及自养条件下小球藻产TAG过程核心代谢网络。采用改进CASOP(Co...以基因工程强化微藻三酰甘油(TAG)积累是实现微藻生物柴油应用化的途径之一。为进行有效的基因工程操作,研究分析了微藻代谢网络,以找出其中对TAG合成重要的反应。构建了异养及自养条件下小球藻产TAG过程核心代谢网络。采用改进CASOP(Computational approach for strain optimization aiming at high productivity)法分析网络中各反应的重要度。结果表明,主要的关键反应包括脂肪酸和TAG的合成反应以及提供NADPH的反应,特别是将NADH转化为NADPH的反应。此外,分析结果也部分解释了环境压力对TAG积累的作用。展开更多
A three-dimensional finite element simulation was carried out to investigate the effects of tunnel construction on nearby pile foundation.The displacement controlled model (DCM) was used to simulate the tunneling-indu...A three-dimensional finite element simulation was carried out to investigate the effects of tunnel construction on nearby pile foundation.The displacement controlled model (DCM) was used to simulate the tunneling-induced volume loss effects.The numerical model was verified based on the results of a centrifuge test and a set of parametric studies was implemented based on this model.There is good agreement between the trend of the results of the centrifuge test and the present model.The results of parametric studies show that the tunnelling-induced pile internal force and deformation depend mainly on the pile?tunnel distance,the pile length to tunnel depth ratio and the volume loss.Two different zones are separated by a 45° line projected from the tunnel springline.Within the zone of influence,the pile is subjected to tensile force and large settlement;whereas outside the zone of influence,dragload and small settlement are induced.It is also established that the impact of tunnelling on a pile group is substantially smaller as compared with a single pile in the same location with the rear pile in a group,demonstrating a positive pile group effect.展开更多
文摘以基因工程强化微藻三酰甘油(TAG)积累是实现微藻生物柴油应用化的途径之一。为进行有效的基因工程操作,研究分析了微藻代谢网络,以找出其中对TAG合成重要的反应。构建了异养及自养条件下小球藻产TAG过程核心代谢网络。采用改进CASOP(Computational approach for strain optimization aiming at high productivity)法分析网络中各反应的重要度。结果表明,主要的关键反应包括脂肪酸和TAG的合成反应以及提供NADPH的反应,特别是将NADH转化为NADPH的反应。此外,分析结果也部分解释了环境压力对TAG积累的作用。
文摘A three-dimensional finite element simulation was carried out to investigate the effects of tunnel construction on nearby pile foundation.The displacement controlled model (DCM) was used to simulate the tunneling-induced volume loss effects.The numerical model was verified based on the results of a centrifuge test and a set of parametric studies was implemented based on this model.There is good agreement between the trend of the results of the centrifuge test and the present model.The results of parametric studies show that the tunnelling-induced pile internal force and deformation depend mainly on the pile?tunnel distance,the pile length to tunnel depth ratio and the volume loss.Two different zones are separated by a 45° line projected from the tunnel springline.Within the zone of influence,the pile is subjected to tensile force and large settlement;whereas outside the zone of influence,dragload and small settlement are induced.It is also established that the impact of tunnelling on a pile group is substantially smaller as compared with a single pile in the same location with the rear pile in a group,demonstrating a positive pile group effect.
