稻-鸭-泥鳅耦合对水稻部分株型结构的影响

为探究稻田养殖能否改善水稻的株型结构从而影响产量的形成和品质的改善,通过田间对比试验,研究水稻分蘖期、孕穗期和齐穗期的部分株型结构。结果表明,与水稻直播处理相比,稻-鸭-泥鳅耦合能促进水稻根系生长、增加植株株高和根冠比,减少无效分蘖,保持后期分蘖稳定;减小水稻茎基宽,增加水稻冠层幅度,保持水稻具有一定的松散度;减小倒3叶和倒4叶叶开角和披垂度,增加剑叶和倒2叶叶开角、披垂度、叶长和叶宽,使水稻叶面积和叶绿素含量较多的分布在植株上部,利于地上部干物质的积累;提高水稻基叶高,减少枯黄叶,减缓叶片衰老,延长叶片的功能时期。本研究结果为水稻产量的提高奠定了一定的理论基础。

Comparative Study on Four Methods for Quick Extraction of Sorghum Genomic DNA

[Objective] This study was to find out a quick,simple,and low-cost method for the extraction of sorghum genomic DNA.[Method] Four plant genomic DNA extraction methods based on CTAB,including liquid nitrogen grinding method（method I）,buffer grinding method（method II）,drying grinding method（method III）and directly grinding method（method IV）,were used to extract the sorghum genomic DNA from leaves;further the quantity and quality of the yielded DNA were detected by gel electrophoresis,SSR-PCR and SRAP-PCR.[Result] These four methods performed no remarkable difference in DNA product.The method I and method II produced DNA with higher purity and better integrity,which,especially from method I,is effective for SRAP-PCR and SSR-PCR.While the DNA extracted via method III and method IV had less integrality and lower purity,and only effective in SSR-PCR.[Conclusion] Enough amount of sorghum genomic DNA to perform tens of PCR could be quickly extracted using all these four methods.The DNA obtained via method I and method II had a broader application spectrum（SRAP,RAPD,ISSR and SSR）than that via method III and method IV which is only proper for PCR targeting small DNA fragments（SSR）.

Comparative Study on the Genomes Between Oryza alta and Oryza latifolia Based on C-Genome

[Objective] Genomic in situ hybridization (GISH) was used to study the relationship between the two CCDD genomes of Oryza alta and Oryza latifolia. [Method] Total DNA of Oryza officinalis (C-genome) was used as a probe for genomic in situ hybridization on metaphase chromosomes from Oryza alta and Oryza latifolia, respectively. [Result] Under certain post-hybridization washing stringencies, C- and D-genome could be distinguished in CCDD genome type; there were huge differences in some CC chromosomes of Oryza alta, Oryza latifolia, and Oryza officinalis. The genome of Oryza latifolia was more original. [Conclusion] Comparative analysis of the Oryza species with identical genome type may facilitate to elucidate the possible approaches to plant genome evolution and species evolution.

Recurrent ischemic strokes in a young celiac woman with MTHFR gene mutation

Celiac disease(CD) is frequently associated with neurological disorders,but very few reports concern the association with ischemic stroke.A 26-year-old woman affected by CD with secondary amenorrhea,carrier of a homozygous 5,10-methylenetetrahydrofolate reductase mutation with hyperhomocysteinemia,was affected by two occipital ischemic strokes within a period of 5 mo.At the time of the second stroke,while she was being treated with folic acid,acetylsalicylic acid and a gluten-free diet,she had left hemianopsia,left hemiparesthesias,and gait imbalance.Brain magnetic resonance imaging showed a subacute right occipital ischemic lesion,which was extended to the dorsal region of the right thalamus and the ipsilateral thalamocapsular junction.Antitransglutaminase and deamidated gliadin peptide antibodies were no longer present,while antinuclear antibodies,antineuronal antibodies and immune circulating complexes were only slightly elevated.Since the patient was taking folic acid,her homocysteine levels were almost normal and apparently not sufficient alone to explain the clinical event.A conventional cerebral angiography showed no signs of vasculitis.Finally,rare causes of occipital stroke in young patients,such as Fabry's disease and mitochondrial myopathy,encephalomyopathy,lactic acidosis and stroke-like symptoms,were also excluded by appropriate tests.Thus,the most probable cause for the recurrent strokes in this young woman remained CD,although the mechanisms involved are still unknown.The two main hypotheses concern malabsorption(with consequent deficiency of vitamins known to exert neurotrophic and neuroprotective effects) and immunemediated mechanisms.CD should be kept in mind in the differential diagnosis of ischemic stroke in young patients.

PCR Amplification，Cloning and Sequencing of RbcL Coding Region in Mesophyll Cell and Bundle Sheath Cell of Sorghum（Sorghum bicolor L．）

The Primacy question addressed in our study is: Is the difterntial expression of rbcL gene in mesophyll cells and in bundle sheath cells related to the sequence of the gene per se?An enzymatic approach was fist established to separate the two groups of cells. Microscopic examination revealed satisfactory separation effect: minimal mutual contamination was found so that no mistake might be introduced into biochemical or molecular biological expeitments using such preparations. CpDNA were isolated from mesophyll cells and from bundle sheath cells and coding region of rbcL gene was obtained from each by PCR ampilfication.Cloning and sequencing were then done on them.Compartive analysis , however, revealed identical sequence, with a length of 1,368 bp, encoding 456 amino acids. Since sequences of the non-coding regions of rbcL gene in masephyll sad bundle sheath have not been obtained, it can not yet be concluded that the differential expression is not related to the sequence itself. Nevertheless,It sesems justifiable to infer that whatever difference there may be between the sequences of rbcL gene in two groups of cells can only be found in the non-coding regions(including promoter and the 3' down stream region).

规模化稻鸭共育对水稻株型结构及产量形成的影响

为探讨规模化稻鸭共育能否改善水稻的株型结构从而影响产量及产量结构的形成,本文通过大田规模化稻鸭共育、常规稻鸭共育和水稻直播的对比试验,研究了水稻分蘖末期和齐穗期的部分株型结构及水稻产量。结果表明:与常规稻鸭共育相比,规模化稻鸭共育减少水稻实际产量和有效穗,水稻部分株型结构各构成因素差异不显著;与水稻直播处理相比,规模化稻鸭共育在分蘖末期和齐穗期降低植株茎基宽,增加冠层幅度,保持植株具有一定松散度;增加剑叶和倒2叶叶开角、披垂度和叶片长度,降低倒3叶和倒4叶叶开角和披垂度,利于植株形成上披下挺的株型结构;增加剑叶、倒2叶和倒3叶叶面积占比,使叶面积较多的分布在上部叶片;促进根系生长,增加基叶高和不同叶序叶片SPAD值,减缓叶片衰老。因此,规模化稻鸭共育优化水稻部分株型结构,有利于提高光能利用效率和干物质积累,从而稳定水稻产量。

On the Fourier Spectra of Distributions in Clifford Analysis

In recent papers by Brackx, Delanghe and Sommen, some fundamental higher dimensional distributions have been reconsidered in the framework of Clifford analysis, eventually leading to the introduction of four broad classes of new distributions in Euclidean space. In the current paper we continue the in-depth study of these distributions, more specifically the study of their behaviour in frequency space, thus extending classical results of harmonic analysis.

Hierarchy Bayesian model based services awareness of high-speed optical access networks

As the speed of optical access networks soars with ever increasing multiple services, the service-supporting ability of optical access networks suffers greatly from the shortage of service awareness. Aiming to solve this problem, a hierarchy Bayesian model based services awareness mechanism is proposed for high-speed optical access networks. This approach builds a so-called hierarchy Bayesian model, according to the structure of typical optical access networks. Moreover, the proposed scheme is able to conduct simple services awareness operation in each optical network unit(ONU) and to perform complex services awareness from the whole view of system in optical line terminal(OLT). Simulation results show that the proposed scheme is able to achieve better quality of services(Qo S), in terms of packet loss rate and time delay.