Although chemotherapy plays a main role in treatment for cancer, gene therapy is thought to be a promising approach of treatment for the future. Thymidine-Kinase gene was delivered into proliferating cells by a retrov...Although chemotherapy plays a main role in treatment for cancer, gene therapy is thought to be a promising approach of treatment for the future. Thymidine-Kinase gene was delivered into proliferating cells by a retroviral mediated gene transfer system in the culture cells. Later treatment with GCV showed definite cytotoxic effect on the HSV-tk modified cells and the effect correlated with the growth rate of cells. The rapid proliferating tumor cells, HR8348, were inhibited more efficiently than slow proliferating cells. In experiment in vivo , the introduced TK producer cells released virus particles continuously into their neighbor and, as compared with the control, GCV treatment exerted remarkable inhibitory effect on the growth of tumor. The inhibition also correlated with the number of injected TK producer cells. With administration of 0.2 ml of #795 tumor cell suspension(100 ng/ml), coinjection of 2 ×106 TK producer cells achieved 37% inhibition while that of 1 ×107 TK producer cells achieved 66% inhibition,though complete regression could not be achieved under such circumstances.Our result suggests that TK gene transfer followed by GCV treatment merits further evaluation as an effective antineoplastic approach.展开更多
Nuclear accidents and terrorism present a serious threat for mass casualty.Accidental or intended radiation exposure leads to radiation-induced gastrointestinal(GI)syndrome.However,currently there are no approved medi...Nuclear accidents and terrorism present a serious threat for mass casualty.Accidental or intended radiation exposure leads to radiation-induced gastrointestinal(GI)syndrome.However,currently there are no approved medical countermeasures for GI syndrome.Thus,developing novel treatments for GI syndrome is urgent.Mesenchymal stem cells(MSCs)derived from bone marrow are a subset of multipotent adult somatic stem cells that have the ability to undergo self-renewal,proliferation and pluripotent differentiation.MSCs have advantages over other stem cells;they can be easily isolated from patients or donors,readily expanded ex vivo,and they possess reparative and immunomodulatory properties.Moreover,MSCs have been shown to be powerful tools in gene therapy and can be effectively transduced with vectors containing therapeutic genes.Therefore,the therapeutic potential of MSCs has been brought into the spotlight for the clinical treatment of GI syndrome.In this review,we discuss the possible role of MSCs in radiation-induced GI syndrome.展开更多
This review discusses recent studies on dendrimer-based nanoparticles in cancer chemotherapy and gene therapy. In order to achieve the high efficacy and low side effects of chemotherapy and gene therapy, it is essenti...This review discusses recent studies on dendrimer-based nanoparticles in cancer chemotherapy and gene therapy. In order to achieve the high efficacy and low side effects of chemotherapy and gene therapy, it is essential to combine the unique features of dendrimers and the specific tumor microenvironment to target delivery and control release of therapeutic agents to tumor tissues and cells. Strategies to design the dendrimer-based delivery system in this review include non-modified dendrimers, dendrimer conjugates, assembled amphiphilic dendrimers, nanohybrid dendrimer carriers and dendrimers with inherent activity. In addition, specific functional groups on these dendrimers as stimuli-responsive system for targeting delivery and controlled release of therapeutic agents are discussed.展开更多
文摘Although chemotherapy plays a main role in treatment for cancer, gene therapy is thought to be a promising approach of treatment for the future. Thymidine-Kinase gene was delivered into proliferating cells by a retroviral mediated gene transfer system in the culture cells. Later treatment with GCV showed definite cytotoxic effect on the HSV-tk modified cells and the effect correlated with the growth rate of cells. The rapid proliferating tumor cells, HR8348, were inhibited more efficiently than slow proliferating cells. In experiment in vivo , the introduced TK producer cells released virus particles continuously into their neighbor and, as compared with the control, GCV treatment exerted remarkable inhibitory effect on the growth of tumor. The inhibition also correlated with the number of injected TK producer cells. With administration of 0.2 ml of #795 tumor cell suspension(100 ng/ml), coinjection of 2 ×106 TK producer cells achieved 37% inhibition while that of 1 ×107 TK producer cells achieved 66% inhibition,though complete regression could not be achieved under such circumstances.Our result suggests that TK gene transfer followed by GCV treatment merits further evaluation as an effective antineoplastic approach.
文摘Nuclear accidents and terrorism present a serious threat for mass casualty.Accidental or intended radiation exposure leads to radiation-induced gastrointestinal(GI)syndrome.However,currently there are no approved medical countermeasures for GI syndrome.Thus,developing novel treatments for GI syndrome is urgent.Mesenchymal stem cells(MSCs)derived from bone marrow are a subset of multipotent adult somatic stem cells that have the ability to undergo self-renewal,proliferation and pluripotent differentiation.MSCs have advantages over other stem cells;they can be easily isolated from patients or donors,readily expanded ex vivo,and they possess reparative and immunomodulatory properties.Moreover,MSCs have been shown to be powerful tools in gene therapy and can be effectively transduced with vectors containing therapeutic genes.Therefore,the therapeutic potential of MSCs has been brought into the spotlight for the clinical treatment of GI syndrome.In this review,we discuss the possible role of MSCs in radiation-induced GI syndrome.
基金supported by the National Natural Science Foundation of China (81601594, 51690153, 21474045 and 21720102005)
文摘This review discusses recent studies on dendrimer-based nanoparticles in cancer chemotherapy and gene therapy. In order to achieve the high efficacy and low side effects of chemotherapy and gene therapy, it is essential to combine the unique features of dendrimers and the specific tumor microenvironment to target delivery and control release of therapeutic agents to tumor tissues and cells. Strategies to design the dendrimer-based delivery system in this review include non-modified dendrimers, dendrimer conjugates, assembled amphiphilic dendrimers, nanohybrid dendrimer carriers and dendrimers with inherent activity. In addition, specific functional groups on these dendrimers as stimuli-responsive system for targeting delivery and controlled release of therapeutic agents are discussed.
