目的评价接种3剂次基因重组乙型肝炎疫苗（recombinant hepatitis B vaccine,HBV-R）5年以上的长期保护效果。方法电子检索《中国生物医学文献数据库》（China Biology Medicine disc,CBMd）、《中国期刊全文数据库》（China National K...目的评价接种3剂次基因重组乙型肝炎疫苗（recombinant hepatitis B vaccine,HBV-R）5年以上的长期保护效果。方法电子检索《中国生物医学文献数据库》（China Biology Medicine disc,CBMd）、《中国期刊全文数据库》（China National Knowledge Infrastructure,CNKI）、《万方全文数据库》（Wanfang Database,WF）、《美国国家医学图书馆数据库》（National Center for Biotechnology Information,NCBI）、《考克兰（Cochrane）协作网图书馆》等数据库,将有关接种HBV-R流行病学保护效果的研究纳入分析。使用Rev Man 5.1软件进行分析。结果共纳入13篇文献和4 882观察对象,有4篇为随机对照试验（random control trial,RCT）,有9篇为前瞻性队列研究（prospective cohort study,PCS）;接种HBV-R后5~20年的乙肝病毒突破感染（breakthrough infection,BI）和（chronic carriage,CC）的累计发生率（accumulative incidence,AI）分别为0.01[95%可信区间（confidence interval,CI）：0.00-0.01],0.00（95%CI：0.00-0.00）。结论全程接种3剂次HBV-R后,可以有效保护免疫功能正常人群且保护效果至少持续20年。展开更多
Objective To assess the correlation between hepatitis B virus (HBV) surface gene mutant infection and hepatitis B (HB) vaccination failure Methods Using sera from 106 infants who were born to HBV carrier mothers a...Objective To assess the correlation between hepatitis B virus (HBV) surface gene mutant infection and hepatitis B (HB) vaccination failure Methods Using sera from 106 infants who were born to HBV carrier mothers and failed in HB immunoprophylaxis, HBV S gene was amplified by PCR, transferred to nylon membranes for Southern blots, and then hybridized with oligonucleotide probes Eleven of non hybridizing samples were used for DNA sequencing Results 93 4% (99/106) of the samples were HBV DNA positive, and 30 3% (30/99) failed to hybridize with at least one of the four probes DNA sequencing confirmed that 10 of the 11 samples had an S gene mutation with amino acid (aa) change The identified mutants included nucleotide (nt) 546T→A(aa131N→T), nt531T→C (aa126I→T), nt491A→C (aa113T→P), nt491T→A (aa113S→T), nt533C→A (aa127P→T), nt581T→A (aa143S→T), nt636A→T (aa161Y→F), and nt679A→C (aa175L→F) The sequence in one mother infant pair was completely the same, with mutations at aa131 and aa161 Conclusions The prevalence of HBV surface mutants is about 30% in the children failing in HB vaccination HBV mutants can infect infants by maternal infant transmission展开更多
文摘目的评价接种3剂次基因重组乙型肝炎疫苗（recombinant hepatitis B vaccine,HBV-R）5年以上的长期保护效果。方法电子检索《中国生物医学文献数据库》（China Biology Medicine disc,CBMd）、《中国期刊全文数据库》（China National Knowledge Infrastructure,CNKI）、《万方全文数据库》（Wanfang Database,WF）、《美国国家医学图书馆数据库》（National Center for Biotechnology Information,NCBI）、《考克兰（Cochrane）协作网图书馆》等数据库,将有关接种HBV-R流行病学保护效果的研究纳入分析。使用Rev Man 5.1软件进行分析。结果共纳入13篇文献和4 882观察对象,有4篇为随机对照试验（random control trial,RCT）,有9篇为前瞻性队列研究（prospective cohort study,PCS）;接种HBV-R后5~20年的乙肝病毒突破感染（breakthrough infection,BI）和（chronic carriage,CC）的累计发生率（accumulative incidence,AI）分别为0.01[95%可信区间（confidence interval,CI）：0.00-0.01],0.00（95%CI：0.00-0.00）。结论全程接种3剂次HBV-R后,可以有效保护免疫功能正常人群且保护效果至少持续20年。
文摘Objective To assess the correlation between hepatitis B virus (HBV) surface gene mutant infection and hepatitis B (HB) vaccination failure Methods Using sera from 106 infants who were born to HBV carrier mothers and failed in HB immunoprophylaxis, HBV S gene was amplified by PCR, transferred to nylon membranes for Southern blots, and then hybridized with oligonucleotide probes Eleven of non hybridizing samples were used for DNA sequencing Results 93 4% (99/106) of the samples were HBV DNA positive, and 30 3% (30/99) failed to hybridize with at least one of the four probes DNA sequencing confirmed that 10 of the 11 samples had an S gene mutation with amino acid (aa) change The identified mutants included nucleotide (nt) 546T→A(aa131N→T), nt531T→C (aa126I→T), nt491A→C (aa113T→P), nt491T→A (aa113S→T), nt533C→A (aa127P→T), nt581T→A (aa143S→T), nt636A→T (aa161Y→F), and nt679A→C (aa175L→F) The sequence in one mother infant pair was completely the same, with mutations at aa131 and aa161 Conclusions The prevalence of HBV surface mutants is about 30% in the children failing in HB vaccination HBV mutants can infect infants by maternal infant transmission
