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大豆卵磷脂碳量子点的光学性能及基因凝聚性能研究
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作者 于子淇 董荣千 +6 位作者 刘佳茗 程振原 王安心 黄宗岱 刘翔 赵海洋 孙婉 《山东化工》 CAS 2024年第13期13-16,21,共5页
碳量子点因良好的光稳定性和低毒性在生物医学领域得到广泛应用,天然的碳源因其成本低、环境友好被广泛用于碳量子点的制备。利用大豆卵磷脂作为碳源通过水热法合成一种大豆卵磷脂基碳量子点。大豆卵磷脂作为一种天然的磷脂具有良好的... 碳量子点因良好的光稳定性和低毒性在生物医学领域得到广泛应用,天然的碳源因其成本低、环境友好被广泛用于碳量子点的制备。利用大豆卵磷脂作为碳源通过水热法合成一种大豆卵磷脂基碳量子点。大豆卵磷脂作为一种天然的磷脂具有良好的药物递送能力,既充当碳源又充当阳离子化剂。通过添加三苯基磷增强其正电性,生成的碳量子点表现出强而稳定的荧光,与未添加三苯基磷组相比,该碳点的尺寸为5 nm,zeta电位为35.8 mV,在与DNA质量比为800∶1时,完全凝聚DNA,具有潜在的DNA递送能力。 展开更多
关键词 碳量子点 大豆卵磷脂 光学性质 基因凝聚
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金黄色葡萄球菌凝聚因子A区基因的克隆表达及表达产物的免疫学特性 被引量:11
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作者 姜晓娟 郝永清 +2 位作者 张爱荣 范鑫 陈晓杰 《中国兽医科学》 CAS CSCD 北大核心 2009年第7期626-631,共6页
采用PCR技术对引起乳牛乳腺炎的金黄色葡萄球菌凝聚因子(ClfA)A区基因进行了扩增和克隆测序,并对测序结果用DNAStar软件进行了抗原表位分析,成功构建了原核表达质粒pET-32a-ClfA A。将其转入宿主菌BL21(DE3),经IPTG诱导表达,证实目的蛋... 采用PCR技术对引起乳牛乳腺炎的金黄色葡萄球菌凝聚因子(ClfA)A区基因进行了扩增和克隆测序,并对测序结果用DNAStar软件进行了抗原表位分析,成功构建了原核表达质粒pET-32a-ClfA A。将其转入宿主菌BL21(DE3),经IPTG诱导表达,证实目的蛋白以可溶形式存在。用His.Bind柱对其纯化后获得了纯度较高的蛋白。纯化后的蛋白与弗氏佐剂乳化后免疫家兔得到了较高效价的抗血清,通过ELISA、试管凝集试验、吞噬调理试验、抗体抗金黄色葡萄球菌黏附能力检测等对表达产物及制备的超免疫血清进行了检测。试验证明目的蛋白具有黏附活性,自制的超免疫血清具有抗金黄色葡萄球菌黏附至牛纤维蛋白原的作用以及吞噬调理作用,所表达的蛋白为ClfA A蛋白。 展开更多
关键词 乳牛 乳腺炎 金黄色葡萄球菌 凝聚因子(ClfA)A区基因
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运用多聚酶链式反应测定啤酒酵母的凝聚性
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作者 贺礼见 彭召海 《啤酒科技》 2003年第7期60-62,共3页
根据絮凝基因的特性,我们运用多聚酶链式反应测定酵母的凝聚能力。应用示踪凝聚基因对30多种储藏酵母菌株进行了凝聚特性的研究。该方法最先应用于检测个别酵母细胞非凝聚性的变异。当酵母突变不絮凝和发酵结束不沉淀时,我们也应用于... 根据絮凝基因的特性,我们运用多聚酶链式反应测定酵母的凝聚能力。应用示踪凝聚基因对30多种储藏酵母菌株进行了凝聚特性的研究。该方法最先应用于检测个别酵母细胞非凝聚性的变异。当酵母突变不絮凝和发酵结束不沉淀时,我们也应用于遗传基因变化的原因研究。该方法不仅速度快,而且具有可靠性和再现性。 展开更多
关键词 多聚酶链式反应 测定 啤酒酵母 凝聚 凝聚基因
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Effects of Spatial Noncommutativity on Energy Spectrum of a Trapped Bose-Einstein Condensate 被引量:1
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作者 LUO You-Hua GE Zi-Ming 《Communications in Theoretical Physics》 SCIE CAS CSCD 2006年第6X期966-968,共3页
In noncommutative space, we examine the problem of a noninteracting and harmonically trapped Bose- Einstein condensate, and derive a simple analytic expression for the effect of spatial noncommutatlvity on energy spec... In noncommutative space, we examine the problem of a noninteracting and harmonically trapped Bose- Einstein condensate, and derive a simple analytic expression for the effect of spatial noncommutatlvity on energy spectrum of the condensate, it indicates that the ground-state energy incorporating the spatial noncommutativity is reduced to a lower level, which depends upon the noncommutativity parameter 8. The gap between the noncommutative space and commutative one for the ground-state level of the condensate should be a signal of spatial noncommutativity. 展开更多
关键词 spatial noncommutativity energy spectrum Bose Einstein condensate
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Phylogenetic relationships between purple and tea-green individuals of the blue swimming crab Portunus trituberculatus by PCR-SSCP analysis 被引量:1
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作者 CHI Da-li GAO Huan +1 位作者 SHEN Song-dong YAN Bin-lun 《Journal of Agricultural Science and Technology》 2009年第1期54-62,共9页
Given the morphological characters of purple crabs identical with that of the tea-green ones except the body color, it is expected that the purple crabs and the tea-green ones were from the same species. In order to i... Given the morphological characters of purple crabs identical with that of the tea-green ones except the body color, it is expected that the purple crabs and the tea-green ones were from the same species. In order to identify the phylogenetic relationships between them at the molecular level, we amplified and compared two conserved mitochondrial gene fragments of purple and tea-green crabs through PCR-SSCP and DNA sequencing analyses. The results showed that there was no distinct variation of DNA bands of both the CO1 and 16srRNA genes detected in polyacrylamide gels through SSCP analysis between purple and tea-green crab samples, and the following sequence analysis of color-different crab individuals presented 99.81% and 99.91% nucleotide sequence identity of the CO1 and 16srRNA genes respectively. These indicated that there was no species or subspecies differentiation between purple and tea-green crabs, meaning that they belonged to the same species, Portunus trituberculatus. 展开更多
关键词 16srRNA body-color COI PCR Portunus trituberculatus SSCP
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Ground State and Single Vortex for Bose-Einstein Condensates in Anisotropic Traps
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作者 XU Zhi-Jun CAI Ping-Gen 《Communications in Theoretical Physics》 SCIE CAS CSCD 2007年第6期1053-1057,共5页
For Bose-Einstein condensation of neutral atoms in anisotropic traps at zero temperature, we present simple analytical methods for computing the properties of ground state and single vortex of Bose-Einstein condensate... For Bose-Einstein condensation of neutral atoms in anisotropic traps at zero temperature, we present simple analytical methods for computing the properties of ground state and single vortex of Bose-Einstein condensates, and compare those results to extensive numerical simulations. The critical angular velocity for production of vortices is calculated for both positive and negative scattering lengths a, and find an analytical expression for the large-N limit of the vortex critical angular velocity for a 〉0, and the critical number for condensate population approaches the point of collapse for a 〈 0, by using approximate variational method. 展开更多
关键词 Bose-Einstein condensation G-P equation wave function vortex formation
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A single nucleotide polymorphism in XRCC4 gene is associated with reduced colorectal cancer susceptibility in female 被引量:1
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作者 Zhang Zhongheng Hu Weiling 《Journal of Medical Colleges of PLA(China)》 CAS 2011年第2期85-93,共9页
Objective: To investigate the association of XRCC4 polymorphic variants at G-1394T (rs6869366) with colorectal cancer susceptibility. Methods: In this hospital-based case-control study, the association of XRCC4 po... Objective: To investigate the association of XRCC4 polymorphic variants at G-1394T (rs6869366) with colorectal cancer susceptibility. Methods: In this hospital-based case-control study, the association of XRCC4 polymorphism with colorectal cancer risk in Chinese population was investigated. In total, 171 patients with colorectal cancer and 171 healthy individuals matched for age and gender were selected. The genomic DNAs of the patients and controls were extracted from peripheral blood and the 300 bp target DNA was amplified with Polymerase Chain Reaction. The products were then digested with restriction endonuclease HinclI, followed by agarose electrophoresis to identify the genotype. Results: We found a significant difference in the frequency of the XRCC4 G-1394T genotype between the colorectal cancer and control groups in female (1/127 vs 8/122, P〈0.05). Those with G/T at XRCC4 G-1394T showed a decreased risk of colorectal cancer susceptibility compared with those with T/T (OR 0.113, 95%CI 0.014-0.932). However, in overall population or in male, there was no significant difference of the distribution between the colorectal cancer and control groups. Conclusion: Our findings with decreased risk of colorectal cancer susceptibility suggested that the G allele of XRCC4 G-1394T were associated in female. 展开更多
关键词 XRCC4 Colorectal cancer Single nucleotide nolvmomhism
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Phase Transition and Superfluid of Photons and Photon Pairs in a Two-Dimensional Optical Microcavity
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作者 张建军 袁建辉 +1 位作者 张俊佩 成泽 《Communications in Theoretical Physics》 SCIE CAS CSCD 2012年第11期672-680,共9页
We analyze the ground-state properties and the excitation spectrum of Bose-Einstein condensates of photons and PPs in a two-dimensional optical microcavity. First, using the variational method, we discuss the ground-s... We analyze the ground-state properties and the excitation spectrum of Bose-Einstein condensates of photons and PPs in a two-dimensional optical microcavity. First, using the variational method, we discuss the ground-state phase transition of the two-component system. We also investigate the energy gap between the ground state and the first excited state. Moreover, by investigating the excitation spectrum, we also illustrate how the superfluid behavior of photons and PPs can be associated with the phase transition of the system. 展开更多
关键词 Bose-Einstein condensation photon pair SUPERFLUID
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Effect of Anisotropic Spin-Orbit Coupling on the Ground State of Bose-Einstein Condensate in an External Potential
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作者 和万全 高日丽 +3 位作者 张培 毕雄伟 潘青山 许世娟 《Communications in Theoretical Physics》 SCIE CAS CSCD 2015年第3期303-307,共5页
Spin-orbit coupled Bosonic atoms confined in external potentials open up new avenues for quantumstate manipulation and will contribute to the design and exploration of novel quantum devices.Here we consider a quasi-tw... Spin-orbit coupled Bosonic atoms confined in external potentials open up new avenues for quantumstate manipulation and will contribute to the design and exploration of novel quantum devices.Here we consider a quasi-two-dimensional spin-orbit coupled Bose-Einstein condensate confined in an external harmonic potential,with emphasis on the effects of anisotropic spin-orbit coupling on the equilibrium ground-state structure of such a system.For the cases with spin-orbit coupling solely in x- or y-axis direction,the ground-state structure can develop to the well-known standing wave phase,in which the two components always form an alternative density arrangement.For a two-dimensional anisotropic spin-orbit coupling,the separated lumps first become bend,then form two rows of stripe structure along y direction with further increasing the strength of spin-orbit coupling in x-direction.Furthermore,the distance between these two rows of stripe structure is also investigated in detail. 展开更多
关键词 spin-orbit coupling Bose-Einstein condensate ground state
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