Background. Atopic dermatitis (AD) is a common chronically relapsing skin disease associated with the activation of T-helper 2 cells. Recent studies have shown that polymorphisms in the genes for interleukin (IL)-4, t...Background. Atopic dermatitis (AD) is a common chronically relapsing skin disease associated with the activation of T-helper 2 cells. Recent studies have shown that polymorphisms in the genes for interleukin (IL)-4, the IL-4 receptor, IL-13, and signal transducer and activator 6 (STAT6) may contribute to susceptibility of AD. To date, no cytokine gene polymorphism study has been conducted on Chinese patients with AD. Aims. To determine whether genetic polymorphisms of the cytokine genes might influence the development of AD. Methods. DNA samples were obtained from 94 patients and 186 control subjects. Using direct sequencing and microsatellite genotyping, we examined 22 polymorphisms in eight cytokine genes including the genes for IL-4,-10,-12B and-13,the IL-4 receptor, tumour necrosis factor (TNF)-α , STAT6, and interferon (IFN)-γ . Results. No significantly different allelic and genotypic distributions of the cytokine gene polymorphisms could be found between patients and controls. Moreover, no association was observed with disease onset, gender, the presence of elevated serum total IgE level or blood eosinophilia. Conclusion. Our study suggests that th e analysed genetic polymorphisms of cytokine genes do not appear to be associate d with AD susceptibility in our Chinese population.展开更多
Vitiligo is an acquired hypomelanotic skin disorder characterised by circumscribed depigmented macules resulting from the loss of functional melanocytes from the cutaneous epidermis and autoimmunity has been suggested...Vitiligo is an acquired hypomelanotic skin disorder characterised by circumscribed depigmented macules resulting from the loss of functional melanocytes from the cutaneous epidermis and autoimmunity has been suggested to play a role in th e pathogenesis of the disease. Recently, an insertion/deletion (I/D) polymorphis m of a 287-base pair repetitive sequence in intron 16 of the angiotensin conver ting enzyme (ACE) gene has been associated with autoimmune disease and with the development of vitiligo. In this study, the distribution of ACE gene I/D genotyp es was investigated in a population of 106 English patients with generalised (no n-segmental) vitiligo and 174 ethnically matched healthy controls using a restriction fragment length polymorphism-polymerase chain reaction genotyping method. No significant difference in the frequ encies of II, ID and DD genotypes was detected between vitiligo patients and con trol subjects (P=0.35). The same result was evident for the genotype distributio n in vitiligo patients with an autoimmune disease and for those without when com pared with controls (P=0.33 and P=0.53, respectively). In addition, the results indicated that the D allele was not significantly over-represented in the group of patients with vitiligo compared with controls (P=0.42) and that this was als o the case for patients with and without associated autoimmunity (P=0.40 and P=0 .62, respectively).展开更多
文摘Background. Atopic dermatitis (AD) is a common chronically relapsing skin disease associated with the activation of T-helper 2 cells. Recent studies have shown that polymorphisms in the genes for interleukin (IL)-4, the IL-4 receptor, IL-13, and signal transducer and activator 6 (STAT6) may contribute to susceptibility of AD. To date, no cytokine gene polymorphism study has been conducted on Chinese patients with AD. Aims. To determine whether genetic polymorphisms of the cytokine genes might influence the development of AD. Methods. DNA samples were obtained from 94 patients and 186 control subjects. Using direct sequencing and microsatellite genotyping, we examined 22 polymorphisms in eight cytokine genes including the genes for IL-4,-10,-12B and-13,the IL-4 receptor, tumour necrosis factor (TNF)-α , STAT6, and interferon (IFN)-γ . Results. No significantly different allelic and genotypic distributions of the cytokine gene polymorphisms could be found between patients and controls. Moreover, no association was observed with disease onset, gender, the presence of elevated serum total IgE level or blood eosinophilia. Conclusion. Our study suggests that th e analysed genetic polymorphisms of cytokine genes do not appear to be associate d with AD susceptibility in our Chinese population.
文摘Vitiligo is an acquired hypomelanotic skin disorder characterised by circumscribed depigmented macules resulting from the loss of functional melanocytes from the cutaneous epidermis and autoimmunity has been suggested to play a role in th e pathogenesis of the disease. Recently, an insertion/deletion (I/D) polymorphis m of a 287-base pair repetitive sequence in intron 16 of the angiotensin conver ting enzyme (ACE) gene has been associated with autoimmune disease and with the development of vitiligo. In this study, the distribution of ACE gene I/D genotyp es was investigated in a population of 106 English patients with generalised (no n-segmental) vitiligo and 174 ethnically matched healthy controls using a restriction fragment length polymorphism-polymerase chain reaction genotyping method. No significant difference in the frequ encies of II, ID and DD genotypes was detected between vitiligo patients and con trol subjects (P=0.35). The same result was evident for the genotype distributio n in vitiligo patients with an autoimmune disease and for those without when com pared with controls (P=0.33 and P=0.53, respectively). In addition, the results indicated that the D allele was not significantly over-represented in the group of patients with vitiligo compared with controls (P=0.42) and that this was als o the case for patients with and without associated autoimmunity (P=0.40 and P=0 .62, respectively).
