Background. Atopic dermatitis (AD) is a common chronically relapsing skin disease associated with the activation of T-helper 2 cells. Recent studies have shown that polymorphisms in the genes for interleukin (IL)-4, t...Background. Atopic dermatitis (AD) is a common chronically relapsing skin disease associated with the activation of T-helper 2 cells. Recent studies have shown that polymorphisms in the genes for interleukin (IL)-4, the IL-4 receptor, IL-13, and signal transducer and activator 6 (STAT6) may contribute to susceptibility of AD. To date, no cytokine gene polymorphism study has been conducted on Chinese patients with AD. Aims. To determine whether genetic polymorphisms of the cytokine genes might influence the development of AD. Methods. DNA samples were obtained from 94 patients and 186 control subjects. Using direct sequencing and microsatellite genotyping, we examined 22 polymorphisms in eight cytokine genes including the genes for IL-4,-10,-12B and-13,the IL-4 receptor, tumour necrosis factor (TNF)-α , STAT6, and interferon (IFN)-γ . Results. No significantly different allelic and genotypic distributions of the cytokine gene polymorphisms could be found between patients and controls. Moreover, no association was observed with disease onset, gender, the presence of elevated serum total IgE level or blood eosinophilia. Conclusion. Our study suggests that th e analysed genetic polymorphisms of cytokine genes do not appear to be associate d with AD susceptibility in our Chinese population.展开更多
Vitiligo is an acquired hypomelanotic skin disorder characterised by circumscribed depigmented macules resulting from the loss of functional melanocytes from the cutaneous epidermis and autoimmunity has been suggested...Vitiligo is an acquired hypomelanotic skin disorder characterised by circumscribed depigmented macules resulting from the loss of functional melanocytes from the cutaneous epidermis and autoimmunity has been suggested to play a role in th e pathogenesis of the disease. Recently, an insertion/deletion (I/D) polymorphis m of a 287-base pair repetitive sequence in intron 16 of the angiotensin conver ting enzyme (ACE) gene has been associated with autoimmune disease and with the development of vitiligo. In this study, the distribution of ACE gene I/D genotyp es was investigated in a population of 106 English patients with generalised (no n-segmental) vitiligo and 174 ethnically matched healthy controls using a restriction fragment length polymorphism-polymerase chain reaction genotyping method. No significant difference in the frequ encies of II, ID and DD genotypes was detected between vitiligo patients and con trol subjects (P=0.35). The same result was evident for the genotype distributio n in vitiligo patients with an autoimmune disease and for those without when com pared with controls (P=0.33 and P=0.53, respectively). In addition, the results indicated that the D allele was not significantly over-represented in the group of patients with vitiligo compared with controls (P=0.42) and that this was als o the case for patients with and without associated autoimmunity (P=0.40 and P=0 .62, respectively).展开更多
文摘结核病(tuberculosis,TB)由结核分枝杆菌(Mycobacterium tuberculosis,MTB)感染引起,是一个全球性的健康问题[1]。近年来,MTB耐药成为TB控制面临的严峻课题[2],尤其是耐药TB(drug-resistant tuberculosis,DR-TB)患者快速增多和广泛流行[3]。DR-TB可导致TB的高患病率、高病死率,造成治疗过程延长和成本增高,还可在社区人群传播引起暴发流行[4]。世界卫生组织(World Health Organization,WHO)的报告显示,2022年全球新发1060万例TB,其中耐多药/利福平耐药TB患者41万例(3.9%),初治患者中占3.3%,复治患者中占17.0%[5]。我国在全球耐多药TB高负担国家中居第三,不同省份MTB耐药率不同。例如,2018年四川省痰涂片阳性TB患者耐药率和耐多药率分别为18.62%和6.43%[6];2015-2016年安徽省则分别为11.42%和7.63%[7]。海口是地处热带的海岛城市,2015-2016年痰涂片阳性肺TB患者耐药率和耐多药率分别为21.0%和6.9%[8],耐药情况不容乐观[9]。本研究旨在分析海口地区DR-TB患者流行病学特征和DR-TB耐药基因分布及其影响因素,为制定相应的防控策略提供参考。
文摘Background. Atopic dermatitis (AD) is a common chronically relapsing skin disease associated with the activation of T-helper 2 cells. Recent studies have shown that polymorphisms in the genes for interleukin (IL)-4, the IL-4 receptor, IL-13, and signal transducer and activator 6 (STAT6) may contribute to susceptibility of AD. To date, no cytokine gene polymorphism study has been conducted on Chinese patients with AD. Aims. To determine whether genetic polymorphisms of the cytokine genes might influence the development of AD. Methods. DNA samples were obtained from 94 patients and 186 control subjects. Using direct sequencing and microsatellite genotyping, we examined 22 polymorphisms in eight cytokine genes including the genes for IL-4,-10,-12B and-13,the IL-4 receptor, tumour necrosis factor (TNF)-α , STAT6, and interferon (IFN)-γ . Results. No significantly different allelic and genotypic distributions of the cytokine gene polymorphisms could be found between patients and controls. Moreover, no association was observed with disease onset, gender, the presence of elevated serum total IgE level or blood eosinophilia. Conclusion. Our study suggests that th e analysed genetic polymorphisms of cytokine genes do not appear to be associate d with AD susceptibility in our Chinese population.
文摘Vitiligo is an acquired hypomelanotic skin disorder characterised by circumscribed depigmented macules resulting from the loss of functional melanocytes from the cutaneous epidermis and autoimmunity has been suggested to play a role in th e pathogenesis of the disease. Recently, an insertion/deletion (I/D) polymorphis m of a 287-base pair repetitive sequence in intron 16 of the angiotensin conver ting enzyme (ACE) gene has been associated with autoimmune disease and with the development of vitiligo. In this study, the distribution of ACE gene I/D genotyp es was investigated in a population of 106 English patients with generalised (no n-segmental) vitiligo and 174 ethnically matched healthy controls using a restriction fragment length polymorphism-polymerase chain reaction genotyping method. No significant difference in the frequ encies of II, ID and DD genotypes was detected between vitiligo patients and con trol subjects (P=0.35). The same result was evident for the genotype distributio n in vitiligo patients with an autoimmune disease and for those without when com pared with controls (P=0.33 and P=0.53, respectively). In addition, the results indicated that the D allele was not significantly over-represented in the group of patients with vitiligo compared with controls (P=0.42) and that this was als o the case for patients with and without associated autoimmunity (P=0.40 and P=0 .62, respectively).
