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RNA干扰技术在肿瘤基因治疗中的研究现状 被引量:11
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作者 田瑞敏 鄢佳程 +2 位作者 王含彦 易芳 陈建业 《重庆医学》 CAS CSCD 北大核心 2013年第7期811-814,共4页
RNA干扰(RNAinterference,RNAi)技术是利用双链RNA(double-strandedRNA,dsRNA),高效、特异性降解细胞内同源信使RNA(messengerRNA,mRNA),从而阻断特定基因表达,使细胞出现靶基因缺失的表型。1998年Fire等[1]首次应用RNAi技... RNA干扰(RNAinterference,RNAi)技术是利用双链RNA(double-strandedRNA,dsRNA),高效、特异性降解细胞内同源信使RNA(messengerRNA,mRNA),从而阻断特定基因表达,使细胞出现靶基因缺失的表型。1998年Fire等[1]首次应用RNAi技术将dsRNA正义链与反义链的混合物注入线虫体内,结果发现注入dsRNA比单独注入单链RNA引起的基因沉默要强得多,并可导致子一代同源基因的沉默。 展开更多
关键词 RNA干扰技术 肿瘤基因 基因功能沉默 基因功能抑制 肿瘤耐药
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Virus-induced gene silencing and its application in plant functional genomics 被引量:23
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作者 HUANG ChangJun QIAN YaJuan +1 位作者 LI ZhengHe ZHOU XuePing 《Science China(Life Sciences)》 SCIE CAS 2012年第2期99-108,共10页
Virus-induced gene silencing is regarded as a powerful and efficient tool for the analysis of gene function in plants because it is simple, rapid and transformation-free. It has been used to perform both forward and r... Virus-induced gene silencing is regarded as a powerful and efficient tool for the analysis of gene function in plants because it is simple, rapid and transformation-free. It has been used to perform both forward and reverse genetics to identify plant functional genes. Many viruses have been developed into virus-induced gene silencing vectors and gene functions involved in development, biotic and abiotic stresses, metabolism, and cellular signaling have been reported. In this review, we discuss the development and application of virus-induced gene silencing in plant functional genomics. 展开更多
关键词 virus-induced gene silencing gene function gene expression
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