An atom gene was cloned from genomic DNA of Scleortinia sclerotiorum by inverse PCR. The evolutionary relationships of S. sclerotiorum and other fungi in atom gene were studied. Results showed that the atom gene from ...An atom gene was cloned from genomic DNA of Scleortinia sclerotiorum by inverse PCR. The evolutionary relationships of S. sclerotiorum and other fungi in atom gene were studied. Results showed that the atom gene from of S. sclerotiorum has a single open reading frame of 4 773 bp and does not include any introns. The derived amino acid sequence consists of 1 590 residues, and it is homologous to all fungal AROM proteins studied so far. The theoretical isoelectric point （pl） and molecular weight （Mw） is 6.5 and 172.66 kD, respectively GC percentage of the arom gene is 44.94. According to the results of searching from CDD and Prosite database, AROM protein of S. sclerotiorum contains five conserve domains： 3-dehydroquinate synthase domain, 3-dehydroquinate dehydratase （3-dehydroquinase） domain, shikimate 5-dehydrogenase domain, shikimate kinase domain, and -enolpyruvylshikimate-3-phosphate synthase （EPSP sythase） domain, and four motifs： two EPSP synthase signatures, dehydroquinase class I active site, shikimate kinase signature. According to the PIR Site Rule PIRSR000514-1, four functionally important amino acid residues are found by alignment. Putative TATA box and CAAT box locate separately in -23 and -77 loci in 5＇ un-translated region, and two loci found in downstream atom gene are likely polyadenylation signals. In addition, phylogeny of atom gene is analyzed.展开更多
文摘An atom gene was cloned from genomic DNA of Scleortinia sclerotiorum by inverse PCR. The evolutionary relationships of S. sclerotiorum and other fungi in atom gene were studied. Results showed that the atom gene from of S. sclerotiorum has a single open reading frame of 4 773 bp and does not include any introns. The derived amino acid sequence consists of 1 590 residues, and it is homologous to all fungal AROM proteins studied so far. The theoretical isoelectric point （pl） and molecular weight （Mw） is 6.5 and 172.66 kD, respectively GC percentage of the arom gene is 44.94. According to the results of searching from CDD and Prosite database, AROM protein of S. sclerotiorum contains five conserve domains： 3-dehydroquinate synthase domain, 3-dehydroquinate dehydratase （3-dehydroquinase） domain, shikimate 5-dehydrogenase domain, shikimate kinase domain, and -enolpyruvylshikimate-3-phosphate synthase （EPSP sythase） domain, and four motifs： two EPSP synthase signatures, dehydroquinase class I active site, shikimate kinase signature. According to the PIR Site Rule PIRSR000514-1, four functionally important amino acid residues are found by alignment. Putative TATA box and CAAT box locate separately in -23 and -77 loci in 5＇ un-translated region, and two loci found in downstream atom gene are likely polyadenylation signals. In addition, phylogeny of atom gene is analyzed.
