DNA expression constructs containing hGH gene directed by bovine αsl-casein gene regulatory sequences were injected into mouse heart or mammary glands in vivo. hGH expression was readily detected by radioimmunoassay ...DNA expression constructs containing hGH gene directed by bovine αsl-casein gene regulatory sequences were injected into mouse heart or mammary glands in vivo. hGH expression was readily detected by radioimmunoassay in milk of the nursing mice throughout lactation. Our results demonstrated the value of this simple and rapid approach in screening gene constructs and identifying transcription regulation sequences in vivo prior to the generation of transgenic animals. For the First time we report the successful expression in mammary gland via intracardial injection.展开更多
Fertilized rabbit eggs injected with SMTPGH gene were cultured in vitro and retention of theinjected gene was studied using PCR technique and nonradioactive labelling methed. In a mediumof TC199 + 10% FCS, three quart...Fertilized rabbit eggs injected with SMTPGH gene were cultured in vitro and retention of theinjected gene was studied using PCR technique and nonradioactive labelling methed. In a mediumof TC199 + 10% FCS, three quarters of the fertilized eggs developed to the blastocyst stage. Noapparent change of the injected gene was found before the 8-cell stage, after which it was eitherintegrated into the chromosome of the host or lost gradually. But finally, the retention rate of theinjected gene should be equal to its integration rate.展开更多
In 2002, 200 kV heavy ion accelerator was operated for 1 300 h for the study on ion implantation modification. The improvement and maintenance of the accelerator were made. 4 species of ion beams(C+, N+, P+ and Fe+) w...In 2002, 200 kV heavy ion accelerator was operated for 1 300 h for the study on ion implantation modification. The improvement and maintenance of the accelerator were made. 4 species of ion beams(C+, N+, P+ and Fe+) were provided.The main experiments are .is follows.1. Synthesis of C-N compounds by high-energy heavy ion irradiation.Diamond-like carbon thin film and graphite carbon samples were implanted with N+ ion beams at room temperature. The energy of implanted ion is 120 keV and 100 keV. The dosage range is from 5×1017 to展开更多
As a newly identified transcription factor in Candida albcians, CaSfl1 has been shown to be involved in cell flocculation and filamentation and in the negative regulation of several genes involved in hyphal growth. In...As a newly identified transcription factor in Candida albcians, CaSfl1 has been shown to be involved in cell flocculation and filamentation and in the negative regulation of several genes involved in hyphal growth. In this study, we constructed Casfl1△/△ mutants and confirmed that deletion of this gene indeed affected cell flocculation and filamentation. In addition, by RT-PCR we found that while Casfl1 repressed the expression of several hypha-specific genes including HWP1, ECE1, ALS1, ALS3, and FLO8, it strongly activated the expression of the heat-shock protein genes HSP30 and HSP90 under certain stress conditions. Therefore, we propose that CaSfl1 can act as both positive and negative regulators, thereby playing a dual role in transcriptional controls in Candida albicans.展开更多
文摘DNA expression constructs containing hGH gene directed by bovine αsl-casein gene regulatory sequences were injected into mouse heart or mammary glands in vivo. hGH expression was readily detected by radioimmunoassay in milk of the nursing mice throughout lactation. Our results demonstrated the value of this simple and rapid approach in screening gene constructs and identifying transcription regulation sequences in vivo prior to the generation of transgenic animals. For the First time we report the successful expression in mammary gland via intracardial injection.
基金This work was supported by National High Technoloqy Grants 86310105.
文摘Fertilized rabbit eggs injected with SMTPGH gene were cultured in vitro and retention of theinjected gene was studied using PCR technique and nonradioactive labelling methed. In a mediumof TC199 + 10% FCS, three quarters of the fertilized eggs developed to the blastocyst stage. Noapparent change of the injected gene was found before the 8-cell stage, after which it was eitherintegrated into the chromosome of the host or lost gradually. But finally, the retention rate of theinjected gene should be equal to its integration rate.
文摘In 2002, 200 kV heavy ion accelerator was operated for 1 300 h for the study on ion implantation modification. The improvement and maintenance of the accelerator were made. 4 species of ion beams(C+, N+, P+ and Fe+) were provided.The main experiments are .is follows.1. Synthesis of C-N compounds by high-energy heavy ion irradiation.Diamond-like carbon thin film and graphite carbon samples were implanted with N+ ion beams at room temperature. The energy of implanted ion is 120 keV and 100 keV. The dosage range is from 5×1017 to
基金the Joint Research Fund for Overseas Natural Science of China (Grant No. 30228001)National Basic Research Program of China (Grant No. 2007CB914401)
文摘As a newly identified transcription factor in Candida albcians, CaSfl1 has been shown to be involved in cell flocculation and filamentation and in the negative regulation of several genes involved in hyphal growth. In this study, we constructed Casfl1△/△ mutants and confirmed that deletion of this gene indeed affected cell flocculation and filamentation. In addition, by RT-PCR we found that while Casfl1 repressed the expression of several hypha-specific genes including HWP1, ECE1, ALS1, ALS3, and FLO8, it strongly activated the expression of the heat-shock protein genes HSP30 and HSP90 under certain stress conditions. Therefore, we propose that CaSfl1 can act as both positive and negative regulators, thereby playing a dual role in transcriptional controls in Candida albicans.
