To analyze the biological effects of space environment,the diversity of genomic DNA between the space flight soybean 194(4126) with phenotype of good yield and good fruit quality induced by space flight and the soybea...To analyze the biological effects of space environment,the diversity of genomic DNA between the space flight soybean 194(4126) with phenotype of good yield and good fruit quality induced by space flight and the soybean with ground control was studied by amplified fragment length polymorphism(AFLP) method,and the polymorphism of space flight soybean 194(4126) was 3.56%.The differences of protein expression of seeds and leaves between the two kinds of soybeans were analysed by two-dimensional electrophoresis,PDQuest software and MALDI-TOF mass spectrometry.Results show that the loss and decrease of protein expression in 194(4126) soybean are subjected to the space fight of seeds,and three special proteins including Dehydrin,MAT1 and ceQORH are identified.It is concluded that the space environment changes the phenotype and genotype of soybeans due to the space flight of seeds.展开更多
To obtain the recombination protein of renalase and prepare the monoclonal antibody, the human renalase gene was amplified from human kidney by specific primer and cloned the DNA fragments into the pET22b. After verif...To obtain the recombination protein of renalase and prepare the monoclonal antibody, the human renalase gene was amplified from human kidney by specific primer and cloned the DNA fragments into the pET22b. After verification of the positive clones, the gene was transformed to E. coli BL21 to express the protein with 6His on C terminal. The Balb/c mouse was immunized with the purified protein to prepare the monoclonal antibody by hybidoma technique. The renalase protein was reconstructed and 2 strains of the hybidoma which can stable secrete renalase were obtained. The monoclonal antibody can both react with the both recombinant and human serum renalase.展开更多
基金Sponsored by the National High Technology Research and Development Program of China(Grant No. 2004AA231071)the Fund of Harbin Institute of Technology (Grant No.HITQNJS.2006.048 and HIT.2000.10)
文摘To analyze the biological effects of space environment,the diversity of genomic DNA between the space flight soybean 194(4126) with phenotype of good yield and good fruit quality induced by space flight and the soybean with ground control was studied by amplified fragment length polymorphism(AFLP) method,and the polymorphism of space flight soybean 194(4126) was 3.56%.The differences of protein expression of seeds and leaves between the two kinds of soybeans were analysed by two-dimensional electrophoresis,PDQuest software and MALDI-TOF mass spectrometry.Results show that the loss and decrease of protein expression in 194(4126) soybean are subjected to the space fight of seeds,and three special proteins including Dehydrin,MAT1 and ceQORH are identified.It is concluded that the space environment changes the phenotype and genotype of soybeans due to the space flight of seeds.
基金the Scientific Research Project of Shanghai Municipal Health Bureau (2008Y034)the Natural Scientific Research Project of Shanghai(05ZR14086)
文摘To obtain the recombination protein of renalase and prepare the monoclonal antibody, the human renalase gene was amplified from human kidney by specific primer and cloned the DNA fragments into the pET22b. After verification of the positive clones, the gene was transformed to E. coli BL21 to express the protein with 6His on C terminal. The Balb/c mouse was immunized with the purified protein to prepare the monoclonal antibody by hybidoma technique. The renalase protein was reconstructed and 2 strains of the hybidoma which can stable secrete renalase were obtained. The monoclonal antibody can both react with the both recombinant and human serum renalase.
