期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
活性污泥微生物解壳聚糖松江菌中菌胶团形成相关大型基因簇的鉴定和分析 被引量:5
1
作者 王晶 高娜 +3 位作者 刘双元 戴景程 刘亚琦 邱东茹 《微生物学通报》 CAS CSCD 北大核心 2019年第8期1946-1953,共8页
【背景】活性污泥法已广泛应用于城市污水和工业废水的处理,微生物菌胶团的形成在污泥通过重力沉淀实现泥水分离和污泥回用的过程中起着重要作用。从西安北石桥污水处理厂活性污泥中分离到一株菌胶团形成菌XHY-A6,经鉴定为解壳聚糖松江... 【背景】活性污泥法已广泛应用于城市污水和工业废水的处理,微生物菌胶团的形成在污泥通过重力沉淀实现泥水分离和污泥回用的过程中起着重要作用。从西安北石桥污水处理厂活性污泥中分离到一株菌胶团形成菌XHY-A6,经鉴定为解壳聚糖松江菌(Mitsuaria chitosanitabida)。【目的】旨在揭示该株解壳聚糖松江菌菌胶团形成相关的基因及其菌胶团形成机制。【方法】结合分子遗传学,包括转座子插入突变技术和遗传互补分析以及基因组学方法分析与菌胶团形成相关的基因和基因簇。【结果】通过转座子插入突变技术获得了两株菌胶团形成缺陷的突变株,转座子插入位点在糖基转移酶(称为gt3)和多糖链长决定蛋白(wzz)基因内,且这两个基因位于一个与菌胶团形成相关的大型基因簇内,该基因簇内还包括与胞外多糖生物合成和分泌相关的基因、epsB2-prsK-psrR-prsT基因以及一个编码PEP-CTERM蛋白A的基因,遗传互补分析证明gt3基因、wzz基因及其下游wzc基因在菌胶团形成过程中是必需的。【结论】松江菌中菌胶团形成和调控机制极可能与活性污泥优势菌动胶菌(Zoogloea)非常相似,即由胞外多糖和PEP-CTERM家族胞外蛋白质共同介导。从武汉二郎庙、汤逊湖和深圳南山污水处理厂活性污泥中分离纯化出松江菌,这些松江菌属细菌可以用于富含几丁质和壳聚糖的市政污水和虾蟹类食品加工废水的净化和资源化利用。 展开更多
关键词 解壳聚糖松江菌 活性污泥 菌胶团形成 胞外多聚物 基因簇分析
原文传递
Assessment of genetic diversity by simple sequence repeat markers among forty elite varieties in the germplasm for malting barley breeding 被引量:5
2
作者 Jun-mei WANG Jian-ming YANG Jing-huan ZHU Qiao-jun JIA Yue-zhi TAO 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2010年第10期792-800,共9页
The genetic diversity and relationship among 40 elite barley varieties were analyzed based on simple sequence repeat (SSR) genotyping data. The amplified fragments from SSR primers were highly polymorphic in the bad... The genetic diversity and relationship among 40 elite barley varieties were analyzed based on simple sequence repeat (SSR) genotyping data. The amplified fragments from SSR primers were highly polymorphic in the badey accessions investigated. A total of 85 alleles were detected at 35 SSR loci, and allelic variations existed at 29 SSR loci. The allele number per locus ranged from 1 to 5 with an average of 2.4 alleles per locus detected from the 40 badey accessions. A cluster analysis based on the genetic similarity coefficients was conducted and the 40 varieties were classified into two groups. Seven malting barley varieties from China fell into the same subgroup. It was found that the genetic diversity within the Chinese malting barley varieties was narrower than that in other barley germplasm sources, suggesting the importance and feasibility of introducing elite genotypes from different origins for malting barley breeding in China. 展开更多
关键词 Barley (Hordeum vu/gare L.) Genetic similarity Simple sequence repeat (SSR) marker Cluster analysis Genetic diversity
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部