Microsatellites or SSRs as powerful genetic markers have widely been used in genetics and evolutionary biology in common wheat. Because of the high polymorphism, newly synthesized hexaploid wheat has been used in the ...Microsatellites or SSRs as powerful genetic markers have widely been used in genetics and evolutionary biology in common wheat. Because of the high polymorphism, newly synthesized hexaploid wheat has been used in the construction of genetic segregation population for SSR markers, However, data on the evolution of microsatellites during the polyploidization event of hexaploid wheat are limited. In this study, 66 pairs of specific to A/B genome SSR patterns among newly synthesized hexaploid wheat, the donor tetraploid wheat and Aegilops tauschii were compared. The results indicated that most SSR markers were conserved during the polyploidization events of newly synthetic hexaploid wheat, from Triticum turgidum and Ae. tauschii. Over 70% A/B genome specific SSR markers could amplify the SSR sequences from the D genome ofAe. tauschii. Most amplified fragments from Ae, tauschii were detected in synthetic hexaploid at corresponding positions with the same sizes and patterns as in its parental Ae. tauschii. This suggested that these SSR markers, specific for A/B genome in common wheat, could amplify SSR products of D genome besides A/B genome in the newly synthesized hexaploid wheat, that is, these SSR primers specific for A/B genome in common wheat were nonspecific for the A/B genome in the synthetic hexaploid wheat. In addition, one amplified Ae. tauschii product was not detected in the newly synthetic hexaploid wheat. An extra-amplified product was found in the newly synthetic hexaploid wheat. These results suggested that caution should be taken when using SSR marker to genotype newly synthetic hexaploid wheat.展开更多
Dear Editor,Although simple sequence repeat（SSR）markers are not new,they are still useful and often used markers in molecular mapping and marker-assisted breeding,particularly in developing countries.However,locus-s...Dear Editor,Although simple sequence repeat（SSR）markers are not new,they are still useful and often used markers in molecular mapping and marker-assisted breeding,particularly in developing countries.However,locus-specific SSR markers could be more useful and informative in wheat breeding and genetic studies.In the present study,221,911 locus-specific SSR markers were designed.Verification of polymorphisms showed that the proportion of polymorphic markers increases with an increase in SSR size.展开更多
基金the project of Scientific Research Foundation for the Returned Overseas Chinese Scholars, New Century Excellent Talents in University (No. NCET-04-0908)Changjiang Scholars and Innovative Research Team in University (No. IRT0 453) of the Chinese Ministry of EducationNational Natural Science Foundation of China (No. 30270804), Education Department and Science and Technology Department of Sichuan Province.
文摘Microsatellites or SSRs as powerful genetic markers have widely been used in genetics and evolutionary biology in common wheat. Because of the high polymorphism, newly synthesized hexaploid wheat has been used in the construction of genetic segregation population for SSR markers, However, data on the evolution of microsatellites during the polyploidization event of hexaploid wheat are limited. In this study, 66 pairs of specific to A/B genome SSR patterns among newly synthesized hexaploid wheat, the donor tetraploid wheat and Aegilops tauschii were compared. The results indicated that most SSR markers were conserved during the polyploidization events of newly synthetic hexaploid wheat, from Triticum turgidum and Ae. tauschii. Over 70% A/B genome specific SSR markers could amplify the SSR sequences from the D genome ofAe. tauschii. Most amplified fragments from Ae, tauschii were detected in synthetic hexaploid at corresponding positions with the same sizes and patterns as in its parental Ae. tauschii. This suggested that these SSR markers, specific for A/B genome in common wheat, could amplify SSR products of D genome besides A/B genome in the newly synthesized hexaploid wheat, that is, these SSR primers specific for A/B genome in common wheat were nonspecific for the A/B genome in the synthetic hexaploid wheat. In addition, one amplified Ae. tauschii product was not detected in the newly synthetic hexaploid wheat. An extra-amplified product was found in the newly synthetic hexaploid wheat. These results suggested that caution should be taken when using SSR marker to genotype newly synthetic hexaploid wheat.
基金supported by the National Natural Science Foundation of China(31270704,31600997)the Yangzhou Key Research and Development Program(YZ2016035)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)
文摘Dear Editor,Although simple sequence repeat（SSR）markers are not new,they are still useful and often used markers in molecular mapping and marker-assisted breeding,particularly in developing countries.However,locus-specific SSR markers could be more useful and informative in wheat breeding and genetic studies.In the present study,221,911 locus-specific SSR markers were designed.Verification of polymorphisms showed that the proportion of polymorphic markers increases with an increase in SSR size.
