凋亡抑制蛋白（inhibitor of apoptosis protein．IAP）是一类在结构上具有同源性的细胞内源性凋亡抑制蛋白家族，目前为止在人类已经发现的IAPs至少有8种。它们能够对抗各种凋亡诱导因素，抵抗细胞凋亡。X-连锁调亡抑制蛋白（X—linked...凋亡抑制蛋白（inhibitor of apoptosis protein．IAP）是一类在结构上具有同源性的细胞内源性凋亡抑制蛋白家族，目前为止在人类已经发现的IAPs至少有8种。它们能够对抗各种凋亡诱导因素，抵抗细胞凋亡。X-连锁调亡抑制蛋白（X—linked inhibitor of apoptosis protein，XIAP）是IAP家族的重要成员，定位于Xq24—25，编码区由7个外显子组成，cDNA全长2540bp，mRNA全长为9kb，但实际编码区仅有1．5kb，XIAP含497个氨基酸，分子量为57kD。展开更多
The nucleax mains attachment regions(MARs) and the binding nuclear matrix proteins in the 5’-flalildng cisacting elements of the humanε-globin gene have been examined. Using in vitro DNA-matrix binding assay,it has ...The nucleax mains attachment regions(MARs) and the binding nuclear matrix proteins in the 5’-flalildng cisacting elements of the humanε-globin gene have been examined. Using in vitro DNA-matrix binding assay,it has been shown that the positive stage-specific regulatory element (ε-PREII, -446bp-419bp) upstream of this gene could specifically associate with the nuclear matrix from K562 cells, indicating thatε-PREII mad be an erythroidspecilic facultstive MAR. In gel mobility shift assay and Southwestern blotting assal an eothroid-specific nuclear matrix protein (ε-NMPk) in K562 cells has been revealed to bind to this positive regulatory element (E-PREII). Furthermore, we demonstrated that the silencer (-392hp -177bp) uP8tream of the humanε-globin gene could associate with the nuclear matrices from K562, HEL and Raji cells. In addition, the nucleax matrix proteins prepared from these three cell lines could also bind to this silencer, suggesting that this silencer element linght be a constitutive nuclear mains attachment region (constitutive MAR). Our results demonstrated that the nucleax madrid and nuclear mains proteins lxilght play an important role in the regulation of the human 5-globin gene expression.展开更多
文摘凋亡抑制蛋白（inhibitor of apoptosis protein．IAP）是一类在结构上具有同源性的细胞内源性凋亡抑制蛋白家族，目前为止在人类已经发现的IAPs至少有8种。它们能够对抗各种凋亡诱导因素，抵抗细胞凋亡。X-连锁调亡抑制蛋白（X—linked inhibitor of apoptosis protein，XIAP）是IAP家族的重要成员，定位于Xq24—25，编码区由7个外显子组成，cDNA全长2540bp，mRNA全长为9kb，但实际编码区仅有1．5kb，XIAP含497个氨基酸，分子量为57kD。
文摘The nucleax mains attachment regions(MARs) and the binding nuclear matrix proteins in the 5’-flalildng cisacting elements of the humanε-globin gene have been examined. Using in vitro DNA-matrix binding assay,it has been shown that the positive stage-specific regulatory element (ε-PREII, -446bp-419bp) upstream of this gene could specifically associate with the nuclear matrix from K562 cells, indicating thatε-PREII mad be an erythroidspecilic facultstive MAR. In gel mobility shift assay and Southwestern blotting assal an eothroid-specific nuclear matrix protein (ε-NMPk) in K562 cells has been revealed to bind to this positive regulatory element (E-PREII). Furthermore, we demonstrated that the silencer (-392hp -177bp) uP8tream of the humanε-globin gene could associate with the nuclear matrices from K562, HEL and Raji cells. In addition, the nucleax matrix proteins prepared from these three cell lines could also bind to this silencer, suggesting that this silencer element linght be a constitutive nuclear mains attachment region (constitutive MAR). Our results demonstrated that the nucleax madrid and nuclear mains proteins lxilght play an important role in the regulation of the human 5-globin gene expression.
