期刊文献+
共找到3篇文章
< 1 >
每页显示 20 50 100
带FLAG标签小鼠HuR真核表达载体的构建及其生物学功能的鉴定 被引量:3
1
作者 李涛 于文燕 +3 位作者 欧小利 刘爱华 梅柱中 姜勇 《解放军医学杂志》 CAS CSCD 北大核心 2011年第4期352-355,共4页
目的构建人抗原R(HuR)的真核表达载体,观察其在NIH3T3细胞中的表达并初步分析其生物学功能。方法提取NIH3T3细胞总RNA并反转录为cDNA,以cDNA为模板PCR扩增得到小鼠HuR编码序列,酶切后克隆至pcDNA3.1-FLAG载体;重组载体经PCR、酶切、测... 目的构建人抗原R(HuR)的真核表达载体,观察其在NIH3T3细胞中的表达并初步分析其生物学功能。方法提取NIH3T3细胞总RNA并反转录为cDNA,以cDNA为模板PCR扩增得到小鼠HuR编码序列,酶切后克隆至pcDNA3.1-FLAG载体;重组载体经PCR、酶切、测序鉴定正确后瞬时转染NIH3T3细胞,采用Western blotting分析HuR在细胞中的表达,并采用Real-time PCR检测HuR过表达对DUSP1mRNA水平的影响。结果成功构建了重组质粒pcDNA3.1-HuR-FLAG,Western blotting显示该质粒能在NIH3T3细胞中高效表达,Real-time PCR结果表明HuR过表达可提高细胞内DUSP1基因的mRNA水平。结论成功构建了带FLAG标签的HuR真核表达载体,该载体能在NIH3T3细胞中有效表达,并具有相应的生物学功能,为深入研究肿瘤细胞中HuR对DUSP1基因表达的调控机制奠定基础。 展开更多
关键词 人抗原R 基因表达 基因转录后调控
下载PDF
植物RNA结合蛋白的研究进展 被引量:7
2
作者 唐蜻 《安徽农业科学》 CAS 北大核心 2010年第1期38-41,共4页
从RNA结合蛋白的鉴定方法,RNA结合蛋白的基序,以及植物RNA结合蛋白在调控叶绿素mRNA的稳定和转录、植物抗逆反应、开花和激素信号传导等过程中的作用等方面介绍了植物RNA结合蛋白的研究进展。
关键词 RNA结合蛋白 基因转录后调控 RNA识别基序
下载PDF
Novel Tuning Buttons in Tomato Fruit Ripening:miRNAs and siRNAs
3
作者 Jinhua ZUO Qing WANG +4 位作者 Zheng JU Dongyan CAO Benzhong ZHU Yunbo LUO Lipu GAO 《Agricultural Science & Technology》 CAS 2016年第7期1541-1545,共5页
Tomato has emerged as an emblematic model plants for fleshy fruit research and tomato fruit ripening is a complex and highly coordinated developmental process.The many physiology and biochemical processes associated w... Tomato has emerged as an emblematic model plants for fleshy fruit research and tomato fruit ripening is a complex and highly coordinated developmental process.The many physiology and biochemical processes associated with tomato fruit ripening require changes in the expression of hundreds to thousands of genes.Gene expression is regulated by transcriptional and post-transcriptional pathways,one of the recently discovered mechanisms in plants was small RNAs mediated gene silencing at post-transcriptional(PTGS) level.Intriguingly,several mi RNAs and endogenous si RNAs were revealed to be involved in the fruit ripening process which opened a new avenue in the field of fleshy fruit biology.This review compiled the most recent advances made in deciphering the regulation functions of mi RNAs and si RNAs in tomato fruit ripening.It also emphasized the new perspectives now possible in the small RNAs regulation research in tomato fruit ripening and senescence. 展开更多
关键词 tomato siRNAs hundreds developmental emphasized Ethylene biochemical endogenous coordinated miRNAs
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部