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大力加强心血管病基因治疗的研究与管理 被引量:1
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作者 汤健 周爱儒 唐朝枢 《中华心血管病杂志》 CAS CSCD 北大核心 2002年第2期95-96,共2页
关键词 心血管病 基因疗法 基因运载体系 基础研究
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Development of a high-content screening method to evaluate the cellular population-based biological activity of new siRNA delivering reagent
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作者 李雅婷 郑宜 +4 位作者 潘德林 徐波 武芸 杨振军 张礼和 《Journal of Chinese Pharmaceutical Sciences》 CAS CSCD 2013年第6期467-474,共8页
Normally, cellular responses to modified siRNAs or new siRNA delivery systems have been studied in group cell behavior by PCR, western blotting and fluorescence microscopy. In this study, we present a novel high-conte... Normally, cellular responses to modified siRNAs or new siRNA delivery systems have been studied in group cell behavior by PCR, western blotting and fluorescence microscopy. In this study, we present a novel high-content screening (HCS) strategy to evaluate a novel delivery system (named CLD) of siRNA therapeutics, with which both the content of intracellular siRNAs and changes in protein expressing levels have been quantified in group cells and cellular population. We also observed that with the better cell uptake, CLD provided siRNA therapeutics (siBraf) better antitumor capability. This novel strategy was proved to be with efficiency, accuracy and high competency to adherent cell lines, thus making siRNA research more simplified. 展开更多
关键词 High-content screening siRNA delivery system Gene knock-down Cellular population ERK signaling pathway
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