Recombinant fowlpox viruses(rFPVs)coexpressing MDV gB and chicken type Ⅱ interferon (IFN-Ⅱ) gene were constructed by using different promoters of Ps and PE/L.rFPVs were selected and purified by blue plaque expressi...Recombinant fowlpox viruses(rFPVs)coexpressing MDV gB and chicken type Ⅱ interferon (IFN-Ⅱ) gene were constructed by using different promoters of Ps and PE/L.rFPVs were selected and purified by blue plaque expressing β-galactosidase.The expression of MDV gB gene was confirmed by indirect immunofluorescence assay.The levels of MDV gB expressed in rFPV-gB-IFN under the control of the synthetic promoter Ps was same as that in rFPV-gB.The expression of IFN-Ⅱ was examined in chicken embryo fibroblast(CEF) by protection against vesicular stomatitis virus-induced cytopathic effect.The levels of IFN expressed in CEF was 640～1280 units/mL.When rFPVs were propagated in CEF,the plaque sizes of rFPV-gB were larger than those of rFPV-gB-IFN,the plaque formation units of rFPV-gB were also more than those of rFPV-gB-IFN.展开更多
To study the therapeutic effectiveness of gene recombinant γ interferon (rIFN γ) in children with myelodysplastic syndrome (MDS),sixteen children with refractory anemia (MDS RA) were divided into two groups of rIFN ...To study the therapeutic effectiveness of gene recombinant γ interferon (rIFN γ) in children with myelodysplastic syndrome (MDS),sixteen children with refractory anemia (MDS RA) were divided into two groups of rIFN γ therapy group and routine therapy group. In rIFN γ therapy group rIFN γ, androgenic hormones and predinison were used. In routine therapy group just androgenic hormones and predinison were used. Courses of treatment in both of the two groups were 3 6 months. Results showed the Europe American curative effect standard the total effective rate was 83.3% in rIFN γ therapy group and 25% in routine therapy group. Difference was significantly X =4.75, P<0.05. The therapy, while compared with routine therapy rIFN γ was more effective. It could keep the patients peripheral blood cell counts in a save square, and length the patients life term and has a bood short term therapeutic effectiveness when rIFNγ was used for the treatment of children with low展开更多
文摘Recombinant fowlpox viruses(rFPVs)coexpressing MDV gB and chicken type Ⅱ interferon (IFN-Ⅱ) gene were constructed by using different promoters of Ps and PE/L.rFPVs were selected and purified by blue plaque expressing β-galactosidase.The expression of MDV gB gene was confirmed by indirect immunofluorescence assay.The levels of MDV gB expressed in rFPV-gB-IFN under the control of the synthetic promoter Ps was same as that in rFPV-gB.The expression of IFN-Ⅱ was examined in chicken embryo fibroblast(CEF) by protection against vesicular stomatitis virus-induced cytopathic effect.The levels of IFN expressed in CEF was 640～1280 units/mL.When rFPVs were propagated in CEF,the plaque sizes of rFPV-gB were larger than those of rFPV-gB-IFN,the plaque formation units of rFPV-gB were also more than those of rFPV-gB-IFN.
文摘To study the therapeutic effectiveness of gene recombinant γ interferon (rIFN γ) in children with myelodysplastic syndrome (MDS),sixteen children with refractory anemia (MDS RA) were divided into two groups of rIFN γ therapy group and routine therapy group. In rIFN γ therapy group rIFN γ, androgenic hormones and predinison were used. In routine therapy group just androgenic hormones and predinison were used. Courses of treatment in both of the two groups were 3 6 months. Results showed the Europe American curative effect standard the total effective rate was 83.3% in rIFN γ therapy group and 25% in routine therapy group. Difference was significantly X =4.75, P<0.05. The therapy, while compared with routine therapy rIFN γ was more effective. It could keep the patients peripheral blood cell counts in a save square, and length the patients life term and has a bood short term therapeutic effectiveness when rIFNγ was used for the treatment of children with low
