The survey on bird communities was conducted by the belt-style method in six different sample plots in the Honghua抏rji Forests area in the northern Inner Mongolia in June 2001 and totally 28 bird species were recorde...The survey on bird communities was conducted by the belt-style method in six different sample plots in the Honghua抏rji Forests area in the northern Inner Mongolia in June 2001 and totally 28 bird species were recorded. Vegetation investigation was carried out in five 10 m×10 m quadrats at each plot. The asymptotic regression function formulae were adopted to identify the relationships between the vegetation coverage and the numbers of bird species and individuals. The analytical results showed that the changes of species number and density of bird as well as the formation of bird communities follow the changes of forest type and the total foliage. Both the number of bird species and their density decreased with the de-crease of total foliage. The similarity of bird community was very low at the breeding time. In the same classification of cluster, no similarity was higher than 0.65, which indicated that the composition of species had a great difference between all the bird communities. The bird breeding density was closely related to forest growth stage. From the bare grassland ecosystem to cli-max ecosystem, the density of bird species showed a gradually increasing trend.展开更多
The molecular basis of color polymorphism in the shells of the pearl oyster Pinctada fucata is largely unknown. We developed a red-shelled family line and used suppression subtractive hybridization （SSH） to screen f...The molecular basis of color polymorphism in the shells of the pearl oyster Pinctada fucata is largely unknown. We developed a red-shelled family line and used suppression subtractive hybridization （SSH） to screen for differentially expressed genes in red- and non-red-shelled pearl oysters. We constructed forward and reverse cDNA subtractive libraries consisting of 2 506 and 797 clones, respectively. Among 343 randomly selected clones in the forward library, 304 sequences were identified in GenBank using BLASTx and BLASTn. Of the 304 sequences, 13 showed no similarity to known sequences and 291 were matched with known genes of the pearl oyster, including shematrin-1, shematrin-2, shematrin-6, shematrin-7, nacrein, nacrein-like protein, aspein for shell matrix protein, glycine-rich protein, mantle gene 5, 28S, EST00031, EST00036, 16S, and COl. In the reverse library, 7 clones were sequenced and analyzed by BLAST. Two sequences shared similarity with EST00036 from the P. fucata subtraction cDNA library, four with the P. fucata mitochondrial gene for 16S rRNA and 1 with P. fucata shematrin-2. We evaluated the expression of 12 genes from the forward library using RT PCR. Two sequences matched with 16S and CO1 so were considered to be false positives. The remaining 10 sequences were differentially expression in the red-shelled pearl oysters. Our results suggest that differential expression of these genes may be related to color variation in the red-shelled family line of the pearl oyster.展开更多
文摘The survey on bird communities was conducted by the belt-style method in six different sample plots in the Honghua抏rji Forests area in the northern Inner Mongolia in June 2001 and totally 28 bird species were recorded. Vegetation investigation was carried out in five 10 m×10 m quadrats at each plot. The asymptotic regression function formulae were adopted to identify the relationships between the vegetation coverage and the numbers of bird species and individuals. The analytical results showed that the changes of species number and density of bird as well as the formation of bird communities follow the changes of forest type and the total foliage. Both the number of bird species and their density decreased with the de-crease of total foliage. The similarity of bird community was very low at the breeding time. In the same classification of cluster, no similarity was higher than 0.65, which indicated that the composition of species had a great difference between all the bird communities. The bird breeding density was closely related to forest growth stage. From the bare grassland ecosystem to cli-max ecosystem, the density of bird species showed a gradually increasing trend.
基金Supported by National High Technology Research and Development Program of China (863 Program) (No. 2006AA10A409)the Knowledge Innovation Program of the South China Sea Institute of Oceanology, Chinese Academy of Sciences (No. SQ200906)+2 种基金the Science and Technology Program of Guangdong Province (No. 2008A020100004)the National Key Technology Research and Development Program (No. 2007BAD29B01-8)the Key Plan for Marine Development of Guangdong Province (No. A200708C01)
文摘The molecular basis of color polymorphism in the shells of the pearl oyster Pinctada fucata is largely unknown. We developed a red-shelled family line and used suppression subtractive hybridization （SSH） to screen for differentially expressed genes in red- and non-red-shelled pearl oysters. We constructed forward and reverse cDNA subtractive libraries consisting of 2 506 and 797 clones, respectively. Among 343 randomly selected clones in the forward library, 304 sequences were identified in GenBank using BLASTx and BLASTn. Of the 304 sequences, 13 showed no similarity to known sequences and 291 were matched with known genes of the pearl oyster, including shematrin-1, shematrin-2, shematrin-6, shematrin-7, nacrein, nacrein-like protein, aspein for shell matrix protein, glycine-rich protein, mantle gene 5, 28S, EST00031, EST00036, 16S, and COl. In the reverse library, 7 clones were sequenced and analyzed by BLAST. Two sequences shared similarity with EST00036 from the P. fucata subtraction cDNA library, four with the P. fucata mitochondrial gene for 16S rRNA and 1 with P. fucata shematrin-2. We evaluated the expression of 12 genes from the forward library using RT PCR. Two sequences matched with 16S and CO1 so were considered to be false positives. The remaining 10 sequences were differentially expression in the red-shelled pearl oysters. Our results suggest that differential expression of these genes may be related to color variation in the red-shelled family line of the pearl oyster.
