Objective:To detect the expression of RECK gene in the highly and low metastatic cell sublines of human osteosarcoma cell HOS and explore its possible roles on the occurrence and metastasis of osteosarcoma.Methods:RT-...Objective:To detect the expression of RECK gene in the highly and low metastatic cell sublines of human osteosarcoma cell HOS and explore its possible roles on the occurrence and metastasis of osteosarcoma.Methods:RT-PCR, gelatin zymography, and matrigel invasion assay were respectively used to evaluate the endogenous expression of RECK mRNA, MMP-2 activation ratio and invasive capacity in the two osteosarcoma cell sublines.Results:The highly metastatic cell group expressed significantly lower mRNA level of RECK than the low metastatic cell group(P < 0.05), but showed higher MMP-2 activation ratio and invasive capacity(P < 0.05 and P < 0.01, respectively).Conclusion:The abnormal low expression of RECK may participate in osteosarcoma invasion and metastasis, and may be a new therapeutic target for osteosarcoma.展开更多
文摘Objective:To detect the expression of RECK gene in the highly and low metastatic cell sublines of human osteosarcoma cell HOS and explore its possible roles on the occurrence and metastasis of osteosarcoma.Methods:RT-PCR, gelatin zymography, and matrigel invasion assay were respectively used to evaluate the endogenous expression of RECK mRNA, MMP-2 activation ratio and invasive capacity in the two osteosarcoma cell sublines.Results:The highly metastatic cell group expressed significantly lower mRNA level of RECK than the low metastatic cell group(P < 0.05), but showed higher MMP-2 activation ratio and invasive capacity(P < 0.05 and P < 0.01, respectively).Conclusion:The abnormal low expression of RECK may participate in osteosarcoma invasion and metastasis, and may be a new therapeutic target for osteosarcoma.
