Trichoderma is a fungal genus of great and demonstrable biotechnological value, but its genome is poorly surveyed compared with other model microorganisms. Due to their ubiquity and rapid substrate colonization, Trich...Trichoderma is a fungal genus of great and demonstrable biotechnological value, but its genome is poorly surveyed compared with other model microorganisms. Due to their ubiquity and rapid substrate colonization, Trichoderma species have been widely used as biocontrol organisms for agriculture, and their enzyme systems are widely used in industry. Therefore, there is a clear interest to explore beyond the phenotype to exploit the underlying genetic systems using functional genomics tools. The great diversity of species within the Trichoderma genus, the absence of optimized systems for its exploration, and the great variety of genes expressed under a wide range of ambient conditions are the main challenges to consider when starting a comprehensive functional genomics study. An initial project started by three Spanish groups has been extended into the project TRICHOEST, funded by the EU (FP5, QLRT-2001-02032) to target the transcriptome analysis of selected Trichoderma strains with biocontrol potential, in conditions related to antagonism, nutrient stress and plant interactions. Once specific conditions were defined, cDNA libraries were produced and used for EST sequencing. Nine strains from seven Trichoderma species have been considered in this study and an important amount of gene sequence data has been generated, analyzed and used to compare the gene expression in different strains. In parallel to sequencing, genomic expression studies were carried out by means of macro-arrays to identify genes expressed in specific conditions. In silico analysis of DNA sequencing data together with macro-array expression results have lead to a selection based on the potential use of the gene sequences. The selected clone sequences were completed and cloned in appropriate vectors to initiate functional analysis by means of expression studies in homologous and heterologous systems.展开更多
The behavior of building materials in constructions of civil structures is influenced by the surrounding moisture and it is a crucial for intensively examined field of the construction physics. Most standard building ...The behavior of building materials in constructions of civil structures is influenced by the surrounding moisture and it is a crucial for intensively examined field of the construction physics. Most standard building materials are characterized by a porous structure, which results in the ability to receive water in a liquid as well as gaseous form in the inner pores. The water fills the storage space of pores under certain conditions; it is transported and transferred back to the surroundings. Many technical studies show that the moisture monitoring is prevailingly based on experiments. Previous calculating methods introduced, e.g., by Glaser, which became the basis for the standard calculations in many European countries in the 1960s, are not always sufficient with respect to the demands of the civil structures. The moisture influences thermo-insulating properties of the material. By a change of the thermo insulation properties of the construction also the thermal and diffusion scheme of the construction is changed and its thermal resistance is decreasing. Faults in the thermo-technical projects occur when thermal conductivity coefficient L values for material in a dry state are substituted.The aim of the research is to determine the capillary conductivity coefficient as a characteristic material moisture parameter of the building materials by the means of a non-destructive method using the experimentally assembled apparatus developed at the Department of Civil Engineering, Brno University of Technology. Keywords： Capillary conductivity coefficient, moisture transfer, EMWR （electromagnetic microwave radiation）, diffusion展开更多
文摘Trichoderma is a fungal genus of great and demonstrable biotechnological value, but its genome is poorly surveyed compared with other model microorganisms. Due to their ubiquity and rapid substrate colonization, Trichoderma species have been widely used as biocontrol organisms for agriculture, and their enzyme systems are widely used in industry. Therefore, there is a clear interest to explore beyond the phenotype to exploit the underlying genetic systems using functional genomics tools. The great diversity of species within the Trichoderma genus, the absence of optimized systems for its exploration, and the great variety of genes expressed under a wide range of ambient conditions are the main challenges to consider when starting a comprehensive functional genomics study. An initial project started by three Spanish groups has been extended into the project TRICHOEST, funded by the EU (FP5, QLRT-2001-02032) to target the transcriptome analysis of selected Trichoderma strains with biocontrol potential, in conditions related to antagonism, nutrient stress and plant interactions. Once specific conditions were defined, cDNA libraries were produced and used for EST sequencing. Nine strains from seven Trichoderma species have been considered in this study and an important amount of gene sequence data has been generated, analyzed and used to compare the gene expression in different strains. In parallel to sequencing, genomic expression studies were carried out by means of macro-arrays to identify genes expressed in specific conditions. In silico analysis of DNA sequencing data together with macro-array expression results have lead to a selection based on the potential use of the gene sequences. The selected clone sequences were completed and cloned in appropriate vectors to initiate functional analysis by means of expression studies in homologous and heterologous systems.
文摘The behavior of building materials in constructions of civil structures is influenced by the surrounding moisture and it is a crucial for intensively examined field of the construction physics. Most standard building materials are characterized by a porous structure, which results in the ability to receive water in a liquid as well as gaseous form in the inner pores. The water fills the storage space of pores under certain conditions; it is transported and transferred back to the surroundings. Many technical studies show that the moisture monitoring is prevailingly based on experiments. Previous calculating methods introduced, e.g., by Glaser, which became the basis for the standard calculations in many European countries in the 1960s, are not always sufficient with respect to the demands of the civil structures. The moisture influences thermo-insulating properties of the material. By a change of the thermo insulation properties of the construction also the thermal and diffusion scheme of the construction is changed and its thermal resistance is decreasing. Faults in the thermo-technical projects occur when thermal conductivity coefficient L values for material in a dry state are substituted.The aim of the research is to determine the capillary conductivity coefficient as a characteristic material moisture parameter of the building materials by the means of a non-destructive method using the experimentally assembled apparatus developed at the Department of Civil Engineering, Brno University of Technology. Keywords： Capillary conductivity coefficient, moisture transfer, EMWR （electromagnetic microwave radiation）, diffusion
