[ Objective] The aim of this study was to provide a method for solving the problems in preparing BAC vector with High-copy plasmid pUC119- Bluelox BAC. [ Method ] With selecting a proper single restriction site, seque...[ Objective] The aim of this study was to provide a method for solving the problems in preparing BAC vector with High-copy plasmid pUC119- Bluelox BAC. [ Method ] With selecting a proper single restriction site, sequences of a single copy BAC vector plasmid were inserted into proper site of High-copy plasmid pUC119 vector. [ Result] The gene sequence of BAC vector lost control function of single copy number in new plasmid pUC119-BAC and was copied through High-copy form. The gene sequence of BAC vector basic function was completely cutted off through single enzyme digestion and the control function of single copy could be recovered by auto-connection. [ Conclusion] The High-copy pUC119-BAC plasmid was used to copy and amplify high copy of basic function gene sequence in BAC vector, besides that it could be used to construct transfer vector of molecular cloned recombinant virus or BAC library.展开更多
Air and space is one of the most intense fields of science and technology competition for powerful countries.This paper focuses on the competition to achieve mastery of air and space,and analyzes the impact of fast de...Air and space is one of the most intense fields of science and technology competition for powerful countries.This paper focuses on the competition to achieve mastery of air and space,and analyzes the impact of fast developing intelligent technologies from six basic contradictions of the war,including hiding and finding,understanding and confusion,network resilience and network degradation,hitting and intercepting,speed of action and decisionmaking,and shaping the perceptions of key crowd.On this basis,aiming at securing competitive advantage in the future,the development directions of intelligent technologies are proposed for the air and space competition.展开更多
The rescue work of our country is facing with huge moral risk, the problem that the waste and the lack of resistance resources exist at the same time commands the realization of the accurate allocation of assistance r...The rescue work of our country is facing with huge moral risk, the problem that the waste and the lack of resistance resources exist at the same time commands the realization of the accurate allocation of assistance resources. Basing on the analysis of the characteristics and hazards of this population, the writer puts forward the suggestions to construct the urban vagrants＇ accurate rescue system with the relevant suggestions： perfecting the relief systern, fulfilling the basic functions of the relief management station, setting up an environment of sound laws and regulations. Take the temporary solution as the way and the permanent cure as the goal, not only achieve the resolve of the matter on surface, but also the matter of essence, enhance the overall viability of the urban wandering population.展开更多
文摘[ Objective] The aim of this study was to provide a method for solving the problems in preparing BAC vector with High-copy plasmid pUC119- Bluelox BAC. [ Method ] With selecting a proper single restriction site, sequences of a single copy BAC vector plasmid were inserted into proper site of High-copy plasmid pUC119 vector. [ Result] The gene sequence of BAC vector lost control function of single copy number in new plasmid pUC119-BAC and was copied through High-copy form. The gene sequence of BAC vector basic function was completely cutted off through single enzyme digestion and the control function of single copy could be recovered by auto-connection. [ Conclusion] The High-copy pUC119-BAC plasmid was used to copy and amplify high copy of basic function gene sequence in BAC vector, besides that it could be used to construct transfer vector of molecular cloned recombinant virus or BAC library.
文摘Air and space is one of the most intense fields of science and technology competition for powerful countries.This paper focuses on the competition to achieve mastery of air and space,and analyzes the impact of fast developing intelligent technologies from six basic contradictions of the war,including hiding and finding,understanding and confusion,network resilience and network degradation,hitting and intercepting,speed of action and decisionmaking,and shaping the perceptions of key crowd.On this basis,aiming at securing competitive advantage in the future,the development directions of intelligent technologies are proposed for the air and space competition.
文摘The rescue work of our country is facing with huge moral risk, the problem that the waste and the lack of resistance resources exist at the same time commands the realization of the accurate allocation of assistance resources. Basing on the analysis of the characteristics and hazards of this population, the writer puts forward the suggestions to construct the urban vagrants＇ accurate rescue system with the relevant suggestions： perfecting the relief systern, fulfilling the basic functions of the relief management station, setting up an environment of sound laws and regulations. Take the temporary solution as the way and the permanent cure as the goal, not only achieve the resolve of the matter on surface, but also the matter of essence, enhance the overall viability of the urban wandering population.
