AIM: To study the blood coagulation response after partial hepatectomy (PH) at transcriptional level. METHODS: After PH of rats, the associated genes with blood coagulation were obtained through reference to the datab...AIM: To study the blood coagulation response after partial hepatectomy (PH) at transcriptional level. METHODS: After PH of rats, the associated genes with blood coagulation were obtained through reference to the databases, and the gene expression changes in rat regenerating liver were analyzed by the Rat Genome 230 2.0 array. RESULTS: It was found that 107 genes were associated with liver regeneration. The initially and totally expressing gene numbers occurring in initiation phase of liver regeneration (0.5-4 h after PH), G0/G1 transition (4-6 h after PH), cell proliferation (6-66 h after PH), cell differentiation and structure-function reconstruction (66-168 h after PH) were 44, 11, 58, 7 and 44, 33, 100, 71 respectively, showing that the associated genes were mainly triggered in the forepart and prophase, and worked at different phases. According to their expression similarity, these genes were classified into 5 groups: only up-, predominantly up-, only down-, predominantly down-, up- and down-regulation, involving 44, 8, 36, 13 and 6 genes, respectively, and the total times of their up- and down-regulation expression were 342 and 253, respectively, demonstrating that the number of the up-regulated genes was more than that of the down- regulated genes. Their time relevance was classified into 15 groups, showing that the cellular physiological and biochemical activities were staggered during liver regeneration. According to gene expression patterns, they were classified into 29 types, suggesting that their protein activities were diverse and complex during liver regeneration.CONCLUSION: The blood coagulation response is enhanced mainly in the forepart, prophase and anaphase of liver regeneration, in which the response in the forepart, prophase of liver regeneration can prevent the bleeding caused by partial hepatectomy, whereas that in the anaphase contributes to the structure-function reorganization of regenerating liver. In the process, 107 genes associated with liver regeneration play an important role.展开更多
To establish a micropropagation system of three Laurencia complex species （Laurencia okamurai, Laurencia tristicha, and Chondrophvcus undulatus） by tissue culture techniques, we studied the regeneration characterist...To establish a micropropagation system of three Laurencia complex species （Laurencia okamurai, Laurencia tristicha, and Chondrophvcus undulatus） by tissue culture techniques, we studied the regeneration characteristics and optimal culture conditions of axenic algal fragments cultured on solid medium and in liquid medium. Regeneration structures were observed and counted regularly under a reverse microscope to investigate the regeneration process, polarity and optimal illumination, and temperature and salinity levels. The results show that in most cultures of the three species, we obtained bud regeneration on solidified medium with 0.5% agar and in liquid medium. Rhizoid-like regeneration was filamentous and developed from the lower cut surface of fragments in L. okamurai, but was discoid and developed from the apical back side of bud regeneration in L. tristicha and C. undulatus. Regeneration polarity was localized to the apical part of algal fronds in all three species, and on fragments cut from the basal part of algae buds could develop from both the upper and the lower cut surfaces. Buds could develop from both the medullary and the cortical portions in L. okamurai and C. undulatus, while in L. tristicha, buds only emerged from the cortex. The optimal culture conditions for L. okamurai were 4 500 lx, 20℃ and 35 （salinity）; for C. undulatus, 4 500 lx, 20℃ and 30; and for L. tristicha, 4 500 lx, 25℃ and 30.展开更多
Ionic liquids(ILs) are eco-friend and recyclable solvents for dissolution of wool keratin, and water is often used as antisolvent to regenerate keratin from IL solution. To recycle the ILs, removing water is the neces...Ionic liquids(ILs) are eco-friend and recyclable solvents for dissolution of wool keratin, and water is often used as antisolvent to regenerate keratin from IL solution. To recycle the ILs, removing water is the necessary step. However, complete removal of the water is energy-intensive and costly. The water in ionic liquids would change the physicochemical properties and cluster structures of the IL and further affect its dissolution behavior on keratin. Here, 1-ethyl-3-methylimidazolium dimethylphosphate([Emim]DMP) was used for experiments due to its good performance on dissolving keratin. The experimental and simulation results showed that the dissolving capability of [Emim]DMP was decreased and the interactions between cation and anion became weaker with water concentration increasing. Furthermore, the dissolution time of wool keratin in [Emim]DMP increased with water content rising. At the same time, the effect of water in ILs on the secondary structure distribution and thermal stability of regenerated keratin was not obvious. In this work, by taking the structures of [Emim]DMP, keratin dissolution time and properties of the regenerated keratin into consideration, a balanced range of water content in [Emim]DMP was determined, which could not only reduce recycling cost but also not affect the dissolution behavior of the IL.展开更多
基金Supported by the National Natural Science Foundation of China, No. 30270673
文摘AIM: To study the blood coagulation response after partial hepatectomy (PH) at transcriptional level. METHODS: After PH of rats, the associated genes with blood coagulation were obtained through reference to the databases, and the gene expression changes in rat regenerating liver were analyzed by the Rat Genome 230 2.0 array. RESULTS: It was found that 107 genes were associated with liver regeneration. The initially and totally expressing gene numbers occurring in initiation phase of liver regeneration (0.5-4 h after PH), G0/G1 transition (4-6 h after PH), cell proliferation (6-66 h after PH), cell differentiation and structure-function reconstruction (66-168 h after PH) were 44, 11, 58, 7 and 44, 33, 100, 71 respectively, showing that the associated genes were mainly triggered in the forepart and prophase, and worked at different phases. According to their expression similarity, these genes were classified into 5 groups: only up-, predominantly up-, only down-, predominantly down-, up- and down-regulation, involving 44, 8, 36, 13 and 6 genes, respectively, and the total times of their up- and down-regulation expression were 342 and 253, respectively, demonstrating that the number of the up-regulated genes was more than that of the down- regulated genes. Their time relevance was classified into 15 groups, showing that the cellular physiological and biochemical activities were staggered during liver regeneration. According to gene expression patterns, they were classified into 29 types, suggesting that their protein activities were diverse and complex during liver regeneration.CONCLUSION: The blood coagulation response is enhanced mainly in the forepart, prophase and anaphase of liver regeneration, in which the response in the forepart, prophase of liver regeneration can prevent the bleeding caused by partial hepatectomy, whereas that in the anaphase contributes to the structure-function reorganization of regenerating liver. In the process, 107 genes associated with liver regeneration play an important role.
基金Supported by the fund from Jiangsu Key Laboratory of Marine Biotechnology (No. 2006HS006)Huaihai Institute of Technology, Lianyungang, China211-Project Funding of Soochow University (No. 14134908)
文摘To establish a micropropagation system of three Laurencia complex species （Laurencia okamurai, Laurencia tristicha, and Chondrophvcus undulatus） by tissue culture techniques, we studied the regeneration characteristics and optimal culture conditions of axenic algal fragments cultured on solid medium and in liquid medium. Regeneration structures were observed and counted regularly under a reverse microscope to investigate the regeneration process, polarity and optimal illumination, and temperature and salinity levels. The results show that in most cultures of the three species, we obtained bud regeneration on solidified medium with 0.5% agar and in liquid medium. Rhizoid-like regeneration was filamentous and developed from the lower cut surface of fragments in L. okamurai, but was discoid and developed from the apical back side of bud regeneration in L. tristicha and C. undulatus. Regeneration polarity was localized to the apical part of algal fronds in all three species, and on fragments cut from the basal part of algae buds could develop from both the upper and the lower cut surfaces. Buds could develop from both the medullary and the cortical portions in L. okamurai and C. undulatus, while in L. tristicha, buds only emerged from the cortex. The optimal culture conditions for L. okamurai were 4 500 lx, 20℃ and 35 （salinity）; for C. undulatus, 4 500 lx, 20℃ and 30; and for L. tristicha, 4 500 lx, 25℃ and 30.
基金supported by the National Natural Science Foundation of China(21576262)the National Natural Science Fund for Distinguished Young Scholars(21425625)+2 种基金International Cooperation and Exchange of the National Natural Science Foundation of China(51561145020)Key Research Program of Frontier Sciences,CAS(QYZDY-SSW-JSC011)“Recruitment of Outstanding Technologist”of Chinese Academy of Sciences
文摘Ionic liquids(ILs) are eco-friend and recyclable solvents for dissolution of wool keratin, and water is often used as antisolvent to regenerate keratin from IL solution. To recycle the ILs, removing water is the necessary step. However, complete removal of the water is energy-intensive and costly. The water in ionic liquids would change the physicochemical properties and cluster structures of the IL and further affect its dissolution behavior on keratin. Here, 1-ethyl-3-methylimidazolium dimethylphosphate([Emim]DMP) was used for experiments due to its good performance on dissolving keratin. The experimental and simulation results showed that the dissolving capability of [Emim]DMP was decreased and the interactions between cation and anion became weaker with water concentration increasing. Furthermore, the dissolution time of wool keratin in [Emim]DMP increased with water content rising. At the same time, the effect of water in ILs on the secondary structure distribution and thermal stability of regenerated keratin was not obvious. In this work, by taking the structures of [Emim]DMP, keratin dissolution time and properties of the regenerated keratin into consideration, a balanced range of water content in [Emim]DMP was determined, which could not only reduce recycling cost but also not affect the dissolution behavior of the IL.
