Nanoparticles have recendy attracted extensive attention in view of their great potential in biomedicine and bioanalytical applications. Single particle detection via light scattering offers a simple and efficient app...Nanoparticles have recendy attracted extensive attention in view of their great potential in biomedicine and bioanalytical applications. Single particle detection via light scattering offers a simple and efficient approach for the size, size distribution, and concentration analysis of nanoparticles. In particular, intrinsic heterogeneity or rare events masked by ensemble averaging can be revealed. However, the sixth power dependence of Rayleigh scattering on particle size makes it very challenging to detect individual nanoparficles of small sizes. This article is intended to provide an overview of recent progress in the development of techniques based on light scattering for the detection of single nanoparticles.展开更多
Understanding the interactions of nanomaterials(NMs) with biomolecules, organelles, cells, and organic tissues at the nano-bio interface can offer important information for their uptake, distribution, translocation, m...Understanding the interactions of nanomaterials(NMs) with biomolecules, organelles, cells, and organic tissues at the nano-bio interface can offer important information for their uptake, distribution, translocation, metabolism and degradation in vitro and in vivo, which can help to precisely tune and design "smart" NMs for biomedical applications. However, probing the interactions at the nano-bio interface, which generally requires dedicated analytical methods and tools, is remarkably complicated due to the dynamically changed nature of the nano-bio interface. Because of the advantages of high spatial resolution, high sensitivity, excellent accuracy, low matrix effects and non-destructiveness, synchrotron radiation(SR)-based analytical techniques have become extremely valuable tools. Herein, we present a comprehensive overview of SR-based techniques for the visualized study of NMs at cellular and subcellular interfaces and their transformation in vitro; the exploration of biodistribution, translocation, metabolism and degradation of NMs in vivo; and clarification of the molecular mechanisms of NMs' reactions with biomolecules. Rapid development of advanced light source means that in situ, real-time analysis of NMs at the nano-bio interface will be achieved.展开更多
基金the National High Technology Research and Development Program of China(863 Program),No.2007AA02Z487the National Natural Science Foundation of China,No.30672043,30772105,20074031+1 种基金the General Program of the Science and Technology Department of Inner Mongolia Autonomous Region,No.2012MS1124the Scientific Research Program in High Education of Inner Mongolia Autonomous Region,No.NJSZY11128~~
基金supported by the National Natural Science Foundation of China (20675070, 20975087, 90913015 & 21027010)Program for New Century Excellent Talents in University (NCET-07-0729)+1 种基金Research Fund for the Doctoral Program of Higher Education of China (20090121120008 & 20090121110009)the National Fund for Fostering Talents of Basic Science (J1030415)
文摘Nanoparticles have recendy attracted extensive attention in view of their great potential in biomedicine and bioanalytical applications. Single particle detection via light scattering offers a simple and efficient approach for the size, size distribution, and concentration analysis of nanoparticles. In particular, intrinsic heterogeneity or rare events masked by ensemble averaging can be revealed. However, the sixth power dependence of Rayleigh scattering on particle size makes it very challenging to detect individual nanoparficles of small sizes. This article is intended to provide an overview of recent progress in the development of techniques based on light scattering for the detection of single nanoparticles.
基金supported by the National Basic Research Program of China(2011CB933403)the State Key Program of National Natural Science Foundation of China(U1432245)the National Natural Science Foundation of China(11475195,11275214,11375211)
文摘Understanding the interactions of nanomaterials(NMs) with biomolecules, organelles, cells, and organic tissues at the nano-bio interface can offer important information for their uptake, distribution, translocation, metabolism and degradation in vitro and in vivo, which can help to precisely tune and design "smart" NMs for biomedical applications. However, probing the interactions at the nano-bio interface, which generally requires dedicated analytical methods and tools, is remarkably complicated due to the dynamically changed nature of the nano-bio interface. Because of the advantages of high spatial resolution, high sensitivity, excellent accuracy, low matrix effects and non-destructiveness, synchrotron radiation(SR)-based analytical techniques have become extremely valuable tools. Herein, we present a comprehensive overview of SR-based techniques for the visualized study of NMs at cellular and subcellular interfaces and their transformation in vitro; the exploration of biodistribution, translocation, metabolism and degradation of NMs in vivo; and clarification of the molecular mechanisms of NMs' reactions with biomolecules. Rapid development of advanced light source means that in situ, real-time analysis of NMs at the nano-bio interface will be achieved.
