The title compound (C28H27NO5S3, Mr= 553. 69) was prepared bythe reaction of a-thiobenzoylthioformmorholine with diethyl acetylene dicarboxylate.The crystal is monoclinic, space group P21/n with a= 9. 160(3), b= 17. 7...The title compound (C28H27NO5S3, Mr= 553. 69) was prepared bythe reaction of a-thiobenzoylthioformmorholine with diethyl acetylene dicarboxylate.The crystal is monoclinic, space group P21/n with a= 9. 160(3), b= 17. 726(3), c=16. 602(3) A ; β= 100. 375(13)°; V=2651. 4(10) A3, Z=4, Dc= 1. 387 g/cm3, μ(MoKa) =0. 319 mm-1, F(000) =1160, R=0. 0428, wR(F2) =0. 0910 for 2438 observed reflections (I>2(I)). X-ray analysis reveals that interatomic distances for C(5)-C(6), C(13)-C(14) and C(21)-C(22) are 1. 331(4), 1. 351(4), 1. 344(4)A respectively, which show that they are normal C=C double bonds. All S-C bondlengths are similar to typical S-C single bonds (1. 75 - 1. 78 A ). The five-membered ring A (C(5) -C(6) -S(2)-C(13) -S(1) ) (Fig. 1) and six-membered ringB (C(14) -C(15) -C(20) -C(21)-C(22)-S(3) ) (Fig. 1) adopt the flat twist conformation. Furthermore, the morpholine ring adopts chair conformtion.展开更多
Objective To assess the prevalence of mitochondrial Trna leu(uuR)gene mt 3243 A→G mutation and ND-1 gene mt 3316 G→A mutation in Chinese type 2 diabetes.Methods 716 randomly selected,unrelated patients with type 2 d...Objective To assess the prevalence of mitochondrial Trna leu(uuR)gene mt 3243 A→G mutation and ND-1 gene mt 3316 G→A mutation in Chinese type 2 diabetes.Methods 716 randomly selected,unrelated patients with type 2 diabetes were screened for the mutation with a PCR-RFLP technique.Results Three individuals with mitochondrial Trna leu(UUR)gene mt 3243 A→G mutation were identified,representing approximately 0.4% of the type 2 patients screened.Further screening of first-degree relatives of these 3 patients identified another 4 affected carriers.In comparison with type 2 diabetic patients without the mutation,these 7 carriers of the mt3243 mutation had:1)an earlier diagnosis age of diabetes(38.0 ±10.1 year vs 53.4± 10.0 year,P＜0.001);2)lower Body Mass Index(BMI)(19.5±2.0 kg/m2vs 24.9± 10.9 kg/m2,P ＜ 0.0001);and 3)lower post-challenge insulin levels(Area under the curve of insulin levels during the Oral Glucose Tolerance Test(OGTT),2946 ± 1647.2 μlU· ml-1 vs 7469 ± 6647.7 μlU·ml-1,P ＜ 0.01).ND-1 gene mt 3316 G→A mutation was found in 16 patients with type 2 diabetes(2.2%)and 5 out 181 controls(2.7%)with normal glucose tolerance.Therefore,the frequency of the mutation was not different in patients and controls.Moreover,clinical characteristics such as age of onset of diabetes,BMI,and insulin levels were not different between diabetic patients with the mt 3316 mutation and those without it.Conclusions In this large cohort of Chinese Type 2 diabetes,the prevalence of mitochondrial Trna leu(UUR)gene mt 3243 A→G mutation was 0.4%,and the ND-1 gene mt 3316 G→A mutation is a polymorphism unrelated to diabetes.展开更多
文摘The title compound (C28H27NO5S3, Mr= 553. 69) was prepared bythe reaction of a-thiobenzoylthioformmorholine with diethyl acetylene dicarboxylate.The crystal is monoclinic, space group P21/n with a= 9. 160(3), b= 17. 726(3), c=16. 602(3) A ; β= 100. 375(13)°; V=2651. 4(10) A3, Z=4, Dc= 1. 387 g/cm3, μ(MoKa) =0. 319 mm-1, F(000) =1160, R=0. 0428, wR(F2) =0. 0910 for 2438 observed reflections (I>2(I)). X-ray analysis reveals that interatomic distances for C(5)-C(6), C(13)-C(14) and C(21)-C(22) are 1. 331(4), 1. 351(4), 1. 344(4)A respectively, which show that they are normal C=C double bonds. All S-C bondlengths are similar to typical S-C single bonds (1. 75 - 1. 78 A ). The five-membered ring A (C(5) -C(6) -S(2)-C(13) -S(1) ) (Fig. 1) and six-membered ringB (C(14) -C(15) -C(20) -C(21)-C(22)-S(3) ) (Fig. 1) adopt the flat twist conformation. Furthermore, the morpholine ring adopts chair conformtion.
文摘Objective To assess the prevalence of mitochondrial Trna leu(uuR)gene mt 3243 A→G mutation and ND-1 gene mt 3316 G→A mutation in Chinese type 2 diabetes.Methods 716 randomly selected,unrelated patients with type 2 diabetes were screened for the mutation with a PCR-RFLP technique.Results Three individuals with mitochondrial Trna leu(UUR)gene mt 3243 A→G mutation were identified,representing approximately 0.4% of the type 2 patients screened.Further screening of first-degree relatives of these 3 patients identified another 4 affected carriers.In comparison with type 2 diabetic patients without the mutation,these 7 carriers of the mt3243 mutation had:1)an earlier diagnosis age of diabetes(38.0 ±10.1 year vs 53.4± 10.0 year,P＜0.001);2)lower Body Mass Index(BMI)(19.5±2.0 kg/m2vs 24.9± 10.9 kg/m2,P ＜ 0.0001);and 3)lower post-challenge insulin levels(Area under the curve of insulin levels during the Oral Glucose Tolerance Test(OGTT),2946 ± 1647.2 μlU· ml-1 vs 7469 ± 6647.7 μlU·ml-1,P ＜ 0.01).ND-1 gene mt 3316 G→A mutation was found in 16 patients with type 2 diabetes(2.2%)and 5 out 181 controls(2.7%)with normal glucose tolerance.Therefore,the frequency of the mutation was not different in patients and controls.Moreover,clinical characteristics such as age of onset of diabetes,BMI,and insulin levels were not different between diabetic patients with the mt 3316 mutation and those without it.Conclusions In this large cohort of Chinese Type 2 diabetes,the prevalence of mitochondrial Trna leu(UUR)gene mt 3243 A→G mutation was 0.4%,and the ND-1 gene mt 3316 G→A mutation is a polymorphism unrelated to diabetes.
