运用投影仪实现直接混色法合成白光实验

论述了用调压器调节投影仪光强度的方法，用三台投影仪经过滤色片产生三种基本色光，进行直接混色法合成白光实验演示的做法和结果．

浅析电视新闻演播员的布光方法

新闻演播室是电视台的一个重要组成部分,新闻播音员在屏幕上面对面地对广大电视观众直接传播新闻,播音员亲切的肖像画面不仅可以增加播音员自身的美,而且还可以提高画面图像的清晰度,使电视新闻达到更好的传播效果,更能贴近观众、感染观众。因此,本文重点对新闻演播室布光进行论述。

浅谈新闻演播室冷热光源性能比较及其应用

根据新闻演播室灯光录像的工作实践经验 。

振动式盲人景物辨知仪的设计与实验研究

振动式盲人景物辨知仪利用电磁振动器的相对振动强度或频率表示颜色,利用电磁振动器的绝对振动强度或频率表示亮度,再结合使用者对扫描顺序的记忆特点,实现景物信息的振动表示。让盲人使用该实验电路,可通过感知振动而感知亮度和颜色,实现一定程度地辨知景物。

LED冰灯的技术要求

本文依据大量实验和示范应用数据,总结LED冰灯的主要技术指标和指导性数据,为LED冰灯在今后的推广应用提供了详细的指导性规范准则。

LED冰雪景观照明技术浅析

冰雪艺术景观是冰雪建筑与灯光艺术的结合体。文章讨论了冰雪景观照明的需求特点和LED冰雪景观照明系统设计方案需要考虑的问题,并根据大量实验和示范应用数据,总结了LED在冰雪景观照明应用上的主要技术指标和指导性数据,为LED冰雪景观照明在今后的推广应用提供了详细的指导性规范准则。

Application of GFP Gene in the Study of Insect-Resistant Transgenic Plants

用合成的cry1Ac基因与绿色荧光蛋白基因 (GFP)构成融合蛋白基因 ,然后和改造的GNA基因构建双价抗虫基因植物表达载体pBGbfg ,经根癌农杆菌介导转化了烟草。在紫外灯照射下 ,观察到转基因植株叶片中有较强的绿色荧光 ;经抗虫试验、PCR、Southernblot和Westernblot等检测 ,表明该重组植物表达载体能够在转基因植物中有效表达外源基因 ,转基因植株绿色荧光的表型与其抗虫性密切相关。从而成功地建立了以绿色荧光蛋白基因与抗虫基因组成的融合基因转化系统 ,简化了抗虫转基因植物筛选程序 ,有助于快速获得双价抗虫转基因植株。

Human Pro-insulin Transgenic Calf Derived from Somatic Cell Nuclear Transfer

The current study was undertaken to evaluate the possibility of producing a human pro-insulin transgenic cow by means of somatic cell nuclear transfer （SCNT）. A double selection system, Neomycin resistance （Neo^r） gene and enhanced green fluorescent protein （EGFP） gene linked through an inner ribosomal entry site （IRES） sequence directed by a Cytomegalovirus （CMV） promoter, was used for enrichment and selection of the transgenic cells and preimplantation embryos. Transgenes were introduced into bovine fetal fibroblast cells （BFF） cultured in vitro through electroporation （900 V/cm, 5 ms）. Transgenic bovine fibroblast cells （TBF） were enriched through addition of G418 in culture medium （800 μg/mL）. Before being used as a nuclear donor, the TBF cells were either cultured in normal conditions （10% FBS） or treated with serum starvation （0.5% FBS for 2-4 days） followed by 10 hours recovery for G1 phase synchronization. Transgenic cloned embryos were produced through GFP-expressing cell selection and SCNT. The results were the percentage of blastocyst development following SCNT was lower using TBF than BFF cells （23.2% VS 35.2%, P 〈 0.05）. No difference in the percentage of cloned blastocysts between the two groups of transgenic nuclear donor of normal and starvation cultures were observed （23.2% VS 18.9%, P 〉 0.05）. Two to four GFP-expressing blastocysts were transferred into the uterus of each synchronised recipient. One pregnancy from of seven recipients （21 embryos） was confirmed by rectum palpation 60 days after embryo transfer and one recipient has given birth to a calf at term. PCR and DNA sequencing analysis confirmed that the calf was produced using human proinsulin transgenic animal.

Localization of Two GFP_tagged Tobacco Plastid Division Protein NtFtsZs in Escherichia coli

Two plastid division genes, NtFtsZ1 and NtFtsZ2 isolated from Nicotiana tabacum L. were fused with gfp and expressed in Escherichia coli . The regular localizations of full length NtFtsZs∶GFP along the filamentous bacteria indicated that the NtFtsZs could recognize the potential division sites in E. coli and be polymerized with heterogeneous FtsZ from bacteria. The overexpression of NtFtsZs ∶ gfp inhibited the division of host strain cells and resulted in the long filamentous bacterial morphology. These results suggested that eukaryotic ftsZs have similar function to their prokaryotic homologs. Meanwhile, the different deletions of motifs of NtFtsZs are also employed to investigate the functions of these proteins in E. coli . The results showed that the C_terminal domains of NtFtsZs were related to the correct localization of NtFtsZs in E. coli and the N_terminal domains of NtFtsZs were responsible for the polymerization of homogeneous and heterogeneous FtsZ proteins. The significance of these results in understanding the functions of NtFtsZs in plastid division were discussed.

Production of Transgenic Pig Clone Embryo Expressed the Red Fluorescent Protein by Using the Somatic Cell Nucleus Transplantation Technology

[Objective] The research aimed to lay the foundation for producing the transgenic clone pig.[Method] The pig fetus fibroblast with the red fluorescent protein（RFP）gene that was transfected by the retrovirus was as the donor of nucleus transplantation.By using the somatic cell cloning technology,the development situation in vitro of clone embryo with RFP was studied.[Result] The fusion rate of RFP transgenic cell was 83.87% which had no significant difference with 80.56% of non-transgenic cell（P0.05）.The blastula rate in vitro of RFP transgenic somatic cell reconstructed embryo was 8.67% which had no significant difference with 6.56% of non-transgenic cell（P0.05）.After the reconstructed embryo of RFP transgenic somatic cell was transplanted into fifteen receptors,there was no conception individual.[Conclusion] The transgenic cell with the red fluorescent protein as the donor could successfully clone the transgenic embryo and obtain the transgenic blastula.

Construction and expression of GFP conjugated MIM-I-BAR

To achieve a visible inverse Bin-amphiphysin-Rvs （I-BAR）domain recombinant of missing in metastasis （MIM） protein,the green fluorescent protein （GFP）encoding gene was cloned at the terminal of MIM-I-BAR as a probe.The DNA was successfully constructed on a 6xHis-tagged prokaryotic expression plasmid.The non-GFP labeled MIM-I-BAR encoding plasmid was also constructed as a control. Being successfully transformed into BL21 （DE3 ）cells,the GFP-conjugated MIM-I-BAR （MIM-I-BAR-GFP ） exhibits strong visible fluorescence,and the expression product can be easily detected by visual inspection, a fluorescence microscope, Western blot or ultraviolet and visible spectrophotometer. Moreover, examination of expression efficiency under various culture conditions revealed that the MIM-I-BAR-GFP gene has a high protein yield at 10 ℃,but not at the culture temperature of 37 ℃.This property is much different from that of the non-fluorescent MIM-I-BAR gene. This optimal expression condition is also proved to be feasible for protein production in midi-scale. The fluorescent recombinant MIM-I-BAR-GFP protein can serve as a useful tool in scientific research, biomedical application and pharmaceutical development.

Comparative Physical Localization of Rice Pib Gene and Its Linked RFLP Markers in Oryza sativa, O. officinalis and Zea mays

Comparative genetic studies have shown that there are widespread synteny and colinearity of the genes among different species within grass family. Rice ( Oryza sativa L.) is a model plant, and analysis of its genome allows us to reveal the common features and the evolutionary rules of the gramineous genomes and accumulate the data for establishment of a common genetic system in the Poaceae. In this study, a rice gene Pib ( 10.3 kb), a map-based cloned gene, and RFLP markers linked with it are used as the tested probes to investigate their homology and physical location among the tested species. Southern blotting analysis showed that there were sequences homologous to Pib in maize genome. Further, Pib was localized onto the chromosomes of O. sativa ssp. indica cv. Guangluai 4, O. officinalis Wall ex Watt and the inbred line of Zea mays cv. Huangzao 4. The results of fluorescence in situ hybridization (FISH) and double-color FISH indicated that a synteny of Pib and RFLP markers linked with Pib existed among the genomes of the three tested species.

溶杆菌SNNU513基因gfp标记及在玉米根部定殖

溶杆菌属细菌在植物病害生物防治中有着广阔的应用潜力。本文以本实验室从中药材远志分离筛选的溶杆菌属新菌株Lysobacter sp.SNNU513为材料,筛选出高效制备该菌株感受态细胞Inoue法,电击转化条件为场强20 kV/cm、电脉冲时间5 ms时可将含绿色荧光蛋白基因gfp的质粒pGLO导入该菌株感受态细胞中,重组菌SNNU513-pGLO能高效、稳定表达绿色荧光蛋白基因,与出发菌株的生长特性、抑菌活性等生物学特性差异不显著。将成功构建的重组菌SNNU513-pGLO用于检测该菌株在玉米根部的定殖规律,结果表明,定殖量从表皮到韧皮部有明显减少趋势。

The developmental fate of green fluorescent mouse embryonic germ cells in chimeric embryos

Primordial germ cells (PGCs), as precursors of mam-malian germ lineage, have been gaining more attention as anew resource of pluripotent stem cells, which bring a greatpossibility to study developmental events of germ cell invitro and at animal level. EG4 cells derived from 10.5 dayspost coitum (dpc) PGCs of l29/svJ strain mouse wereestablished and maintained in an undifferentiated state.With an attempt to study the differentiation capability ofEG4 cells with a reporter protein: green fluorescence pro-tein, and the possible application of EG4 cells in the re-search of germ cell development, we have generated severalEG4-GFP cell lines expressing enhanced green fluorescenceprotein (EGFP) and still maintaining typicaI characteris-tics of pluripotent stem cells. Then, the differentiation ofEG4-GFP cells in vitro as well as their developmental fatein chimeric embryos which were produced by aggregatingEG4-GFP cells to 8-cell stage embryos were studied. Theresults showed that EG4 cells carrying green fluorescencehave a potential use in the research of germ cell develop-ment and other related studies.

Red Emission of Eu（lll） Complex Based on 1-（7-（ferf-butyl）-g-ethyl-gH- carbazol-2-yl）-4,4,4-trifluorobutane-l,3-dione Excited by Blue Light

A new Eu（III） complex, EuL3（phen）, was synthesized, where L is the abbreviation of de- protonated 1-（7-（tert-butyl）-9-ethyl-gH-carbazol-2-yl）-4,4,4-trifluorobutane-l,3-dione （HL）, phen is the abbreviation of 1,10-phenanthroline. The Eu（III） complex was characterized by element analysis, IR, 1H NMR, UV-visible absorption spectroscopy, thermogravimetric anal- ysis （TGA）, and photoluminescence measurements （PL）. TGA shows that thermal stability of the complex is up to 325 ~C. PL measurement indicates that the Eu（III） complex exhibits intense red-emission and extends their excitation bands to visible region. LEDs device was successfully fabricated by precoating complex EuL3 （phen） onto 460 nm blue-emitting InGaN chip. The emission of device shows that the complex can act as red phosphor in combination with 460 nm blue-emitting chips. This europium complex based on 1-（7-（tert-butyl）-9- ethyl-9H-carbazol-2-yl）-4,4,4-trifluorobutane-l,3-dione is a kind of interesting red-emitting material excited by blue light, which could avoid the damage of excitation by UV light.

Ameliorated stress related proteins are associated with improved cardiac function by sarcoplasmic reticulum calcium ATPase gene transfer in heart failure

Background Previous studies showed that overexpression of sarco-endoplasmic retieulum calcium ATPase （SERCA2a） in a variety of heart failure （HF） models was associated with greatly enhanced cardiac performance. However, it still undefined the effect of SERCA2a overexpression on the systemic inflammatory response and neuro-hormonal factors. Methods A rapid right ventricular pacing model of experimental HF was used in beagles. Then the animals underwent recombinant adeno-associated vires 1 （rAAV1） mediated gene trans- fection by direct intra-myocardium injection. HF animals were randomized to receive the SERCA2a gene, enhanced green fluorescent protein （control） gene, or equivalent phosphate buffered saline. Thirty days after gene delivery, the cardiac function was evaluated by echocardiographic testing. The protein level of SERCA2a was measured by western blotting. The proteomic analysis of left ventricular （LV） sample was determined using two-dimensional （2-D） gel el^ctrophoresis and MALDI-TOF-MS. The serum levels of the systemic inflammatory and neuro-hormonal factors were assayed using radioimmunoassay kits. Results The cardiac function improved after SERCA- 2a gene transfer due to the significantly increased SERCA2a protein level. Beagles treated with SERCA2a had significantly decreased serum levels of the inflammatory markers （interleukin-6 and tumor necrosis factor-a） and neuro-hormonal factors （brain natriuretic peptide, endothelin-1 and angiotensin II） compared with HF animals. The myocardial proteomic analysis showed that haptoglobin heavy chain, heat shock protein （alpha-crystallin-related, B6） were down-regulated, and galectin-1 was up-regulated in SERCA2a group compared with HF group, companied by up-regulated contractile proteins and NADH dehydrogenase. Conclusions These findings demonstrate that regional intramyocardial injections of rAAV 1-SERCA2a vectors may improve global LV function, correlating with reverse activation of the systemic inflammatory, excessive neuroendocrine factors and the stress-associated myocardial proteins, suggesting that the beneficial effects of SERCA2a gene transfer may involve the attenuation of stress-associated reaction.

Antitumor activity of mutant bacterial cytosine deaminase gene for colon cancer

AIM： To evaluate bacterial cytosine deaminase （bCD） mutant D314A and 5-fluorocytosine （5-FC） for treatment of colon cancer in a mouse model. METHODS： Recombinant lentivirus vectors that contained wild-type bCD gene （bCDwt）, and bCD mutant D314A gene （bCD-D314A） with green fluorescence protein gene were constructed and used to infect hu- man colon carcinoma LoVo cells, to generate stable transfected cells, LoVo/null, LoVo/bCDwt or LoVo/bCD- D314A. These were injected subcutaneously into Balb/c nude mice to establish xenograft models. Two weeks post-LoVo cell inoculation, PBS or 5-FC （500 mg/kg） was administered by intraperitoneal （i.p.） injection once daily for 14 d. On the day after LoVo cell injection, mice were monitored daily for tumor volume and survival. RESULTS： Sequence analyses confirmed the construction of recombinant lentiviral plasmids that contained bCDwt or bCD-D314A. The lentiviral vector had high elficacy for gene delivery, and RT-PCR showed that bCDwt or bCD-D314A gene was transferred to LoVo cells. Among these treatment groups, gene delivery or 5-FC administration alone had no effect on tumor growth. However, bCDwt/5-FC or bCD-D314A/5-FC treatment inhibited tumor growth and prolonged survival of mice significantly （P 〈 0.05）. Importantly, the tumor volume in the bCD-D314A/5-FC-treated group was lower than that in the bCDwt/5-FC group （P 〈 0.05）, and bCD- D314A plus 5-FC significantly prolonged survival of mice in comparison with bCDwt plus 5-FC （P 〈 0.05）. CONCLUSION： The bCD mutant D314A enhanced significantly antitumor activity in human colon cancer xenograft models, which provides a promising approach for human colon carcinoma therapy.

Cloning and Characterization of Phytochrome A Gene Fa PHYA from Tall Fescue

The sequence fragment of PHYA, obtained from transcriptome sequencing,was used as the template, and the full-length c DNA sequence of PHYA gene in tall fescue was amplified using 3＇RACE and 5＇RACE techniques. The c DNA sequence of PHYA gene has a complete open reading frame（ORF, 293-6 682 bp）, and it encodes a protein composed of 1 129 amino acids. The N-terminal of Fa PHYA is composed of GAF and Phytochrome domains, and its C-terminal contains two repeated PAS domains, one histidine kinase A domain and one histidine kinase-like ATPase domain. The homology analysis showed that the amino acid sequences of Fa PHYA of tall fescue and PHYAs of gramineous plants have higher homologies（85%）, indicating close genetic relationships. However, the homologies between FaPHYA of tall fescue and PHYAs of monocotyledons are lower, indicating far genetic relationships.

Analytical Expression of Ground State of Double-Well Bose-Einstein Condensates

We derive an approximate analytical expression for the ground state of double-well BEC＇s, which reproduces highly accurately the numerical solution for the whole parameter regimes of the two-body repulsive interaction strength, the total number of atoms, and the hopping parameter.

Efficient production of transgenic chickens using self-inactive HIV-based lentiviral vectors

We demonstrated the simple and effective production of transgenic chickens, in which the enhanced green fluorescence protein （EGFP） was expressed by using third-generation self-inactive HIV-based lentiviral vectors. In our experiments, lentiviruses were injected into 204 fertilized eggs, from which 30 （15%） chickens were hatched. The exogenous gene was detected in the genomes of 16 out of 30 （53%） chickens. The green fluorescence signal was observed directly in various body parts, and was particularly significant in the testes. The transgenes were also found in the offspring of these chickens. The results indicate that HIV-based lentiviral vectors can be used to generate transgenic birds economically and effectively [ Current Zoology 55 （5）： 383 - 387,2009].