目的:探讨湿性脂肪干细胞辅助自体颗粒脂肪注射移植术的有效性。方法:使用一次性注射器吸脂20 m L,使其自然悬浮,将注射器下层水分排出,对颗粒脂肪进行注射移植,对注射完成后出现的损伤、血运以及操作时间进行分析。结果:开展术后随访,8...目的:探讨湿性脂肪干细胞辅助自体颗粒脂肪注射移植术的有效性。方法:使用一次性注射器吸脂20 m L,使其自然悬浮,将注射器下层水分排出,对颗粒脂肪进行注射移植,对注射完成后出现的损伤、血运以及操作时间进行分析。结果:开展术后随访,80例患者术后均没有出现并发症,对外观很满意。患者手术后脂肪坏死发生率低于1%。结论:湿性脂肪干细胞与自体脂肪注射移植技术相结合,可以一次性完成操作,操作简单、安全、快速、经济、不易引起并发症。展开更多
The matrix metalloproteinase (MMP) stromelysin-3 (ST3) has long been implicated to play an important role in extracellular matrix (ECM) remodeling and cell fate determination during normal and pathological processes. ...The matrix metalloproteinase (MMP) stromelysin-3 (ST3) has long been implicated to play an important role in extracellular matrix (ECM) remodeling and cell fate determination during normal and pathological processes. However like other MMPs, the molecular basis of ST3 function in vivo remains unclear due to the lack of information on its physiological substrates. Furthermore, ST3 has only weak activities toward all tested ECM proteins. Using thyroid hormone-dependent Xenopus laevis metamorphosis as a model, we demonstrated previously that ST3 is important for apoptosis and tissue morphogenesis during intestinal remodeling. Here, we used yeast two-hybrid screen with mRNAs from metamorphosing tadpoles to identify potential substrate of ST3 during development. We thus isolated the 37 kd laminin receptor precursor (LR). We showed that LR binds to ST3 in vitro and can be cleaved by ST3 at two sites distinct from where other MMPs cleave. Through peptide sequencing, we determined that the two cleavage sites are in the extracellular domain between the transmembrane domain and laminin binding sequence. Furthermore, we demon strated that these cleavage sites are conserved in human LR. These results together with high levels of human LR and ST3 expression in carcinomas suggest that LR is a likely in vivo substrate of ST3 and that its cleavage by ST3 may alter cell-extracellular matrix interaction, thus, playing a role in mediating the effects of ST3 on cell fate and behavior ob- served during development and pathogenesis.展开更多
文摘目的:探讨湿性脂肪干细胞辅助自体颗粒脂肪注射移植术的有效性。方法:使用一次性注射器吸脂20 m L,使其自然悬浮,将注射器下层水分排出,对颗粒脂肪进行注射移植,对注射完成后出现的损伤、血运以及操作时间进行分析。结果:开展术后随访,80例患者术后均没有出现并发症,对外观很满意。患者手术后脂肪坏死发生率低于1%。结论:湿性脂肪干细胞与自体脂肪注射移植技术相结合,可以一次性完成操作,操作简单、安全、快速、经济、不易引起并发症。
文摘The matrix metalloproteinase (MMP) stromelysin-3 (ST3) has long been implicated to play an important role in extracellular matrix (ECM) remodeling and cell fate determination during normal and pathological processes. However like other MMPs, the molecular basis of ST3 function in vivo remains unclear due to the lack of information on its physiological substrates. Furthermore, ST3 has only weak activities toward all tested ECM proteins. Using thyroid hormone-dependent Xenopus laevis metamorphosis as a model, we demonstrated previously that ST3 is important for apoptosis and tissue morphogenesis during intestinal remodeling. Here, we used yeast two-hybrid screen with mRNAs from metamorphosing tadpoles to identify potential substrate of ST3 during development. We thus isolated the 37 kd laminin receptor precursor (LR). We showed that LR binds to ST3 in vitro and can be cleaved by ST3 at two sites distinct from where other MMPs cleave. Through peptide sequencing, we determined that the two cleavage sites are in the extracellular domain between the transmembrane domain and laminin binding sequence. Furthermore, we demon strated that these cleavage sites are conserved in human LR. These results together with high levels of human LR and ST3 expression in carcinomas suggest that LR is a likely in vivo substrate of ST3 and that its cleavage by ST3 may alter cell-extracellular matrix interaction, thus, playing a role in mediating the effects of ST3 on cell fate and behavior ob- served during development and pathogenesis.
