Erythropoietin (EPO) is a glycoprotein with three N-glycosylation sites and one O-glycosylation sites. According to the reference, all of the N-glyco-chains were acid complex type. The amount of sialic acid in rhEPO w...Erythropoietin (EPO) is a glycoprotein with three N-glycosylation sites and one O-glycosylation sites. According to the reference, all of the N-glyco-chains were acid complex type. The amount of sialic acid in rhEPO was determined and two N-glycosylation sites on it were confirmed by matrix-assisted laser desorption time of flight-time of flight mass spectrometry (MALDI-TOF-TOFMS) in this paper. The total amount of sialic acid in rhEPO accounts for 12.97%. Two N-glycosylation sites (Asn38,Asn83) were identified successfully.展开更多
目的 :为有效缩短阳性血培养中常见细菌药物敏感性(药敏)试验的报告周转时间,探讨在基质辅助激光解析电离飞行时间质谱仪(matrix assisted laser desorption ionization time of flight mass spectrometry,MALDI-TOF MS)直接鉴定结果的...目的 :为有效缩短阳性血培养中常见细菌药物敏感性(药敏)试验的报告周转时间,探讨在基质辅助激光解析电离飞行时间质谱仪(matrix assisted laser desorption ionization time of flight mass spectrometry,MALDI-TOF MS)直接鉴定结果的基础上,对阳性血培养中的常见细菌直接进行药敏试验的可行性。方法:应用MALDI-TOF MS直接鉴定阳性血培养中的细菌,对其中能够准确鉴定的常见细菌直接进行纸片扩散法(Kirby-Bauer法,K-B法)和自动微量稀释测最小抑菌浓度(minimum inhibitory concentration,MIC)法药敏试验(直接药敏试验)。同时,将血培养阳性细菌按常规操作流程转种至合适的培养基,培养过夜后获得的纯菌落再进行K-B法和MIC法常规药敏试验。将直接药敏试验结果与常规药敏试验结果进行比对。结果:以常规药敏试验结果为标准,革兰阳性球菌的K-B法和MIC法直接药敏试验结果与之符合率分别为98.5%和98.4%,小误差率均为0.9%,大误差率分别为0.6%和0.7%;革兰阴性杆菌的K-B法和MIC法直接药敏试验结果的符合率分别为97.8%和98.1%,小误差率分别为1.4%和1.1%,大误差率分别为0.8%和0.9%。以血培养阳性报警为起始时间,阳性血培养中细菌直接药敏试验的报告周转时间约为24 h,而常规药敏试验的报告周转时间约需48 h。结论:阳性血培养中常见细菌的直接药敏试验结果与常规药敏试验结果高度相符,且直接药敏试验的报告周转时间可提前至24 h。因此,结合MALDI-TOF MS快速鉴定的结果,对阳性血培养中的常见细菌直接进行药敏试验可在临床微生物室中推广应用,为临床及时、合理和有效的抗生素治疗提供可靠依据。展开更多
目的探讨基质辅助激光解吸附电离飞行时间质谱(matrix assisted laser desorption ionization ti me of flight mass spectrometry,MALDI-TOF-MS)对宫颈鳞癌(squamous carcinoma of thecervix,SCC)组织切片直接分析,获取宫颈鳞癌早期浸...目的探讨基质辅助激光解吸附电离飞行时间质谱(matrix assisted laser desorption ionization ti me of flight mass spectrometry,MALDI-TOF-MS)对宫颈鳞癌(squamous carcinoma of thecervix,SCC)组织切片直接分析,获取宫颈鳞癌早期浸润的分子标志物的有效性。方法利用超微量点样枪将化学基质直接点样到宫颈鳞癌组织切片上,然后利用基质辅助激光解吸附电离飞行时间质谱直接扫描宫颈鳞癌组织切片,分析宫颈鳞癌组织蛋白质分子。结果获得的25种蛋白质质谱峰强度比较,差异有显著意义(P<0.05)。其中7种差异蛋白质有分类意义,其质/核(M/Z)分别为:m/z3433,m/z4786,m/z10092,m/z10835,m/z15114,m/z15854和m/z16345。差异蛋白质m/z3433,m/z15114,m/z15854在宫颈鳞癌鳞状上皮组织高度表达,可作为宫颈鳞癌早期诊断的标志蛋白;差异蛋白质m/z16345在宫颈鳞癌的间质组织高度表达,可作为宫颈鳞癌早期间质浸润和扩散诊断的标志蛋白。结论利用基质辅助激光解吸附电离飞行时间质谱技术成功获取一组差异蛋白质。该差异蛋白组可望作为宫颈鳞癌早期诊断的标志蛋白。展开更多
文摘Erythropoietin (EPO) is a glycoprotein with three N-glycosylation sites and one O-glycosylation sites. According to the reference, all of the N-glyco-chains were acid complex type. The amount of sialic acid in rhEPO was determined and two N-glycosylation sites on it were confirmed by matrix-assisted laser desorption time of flight-time of flight mass spectrometry (MALDI-TOF-TOFMS) in this paper. The total amount of sialic acid in rhEPO accounts for 12.97%. Two N-glycosylation sites (Asn38,Asn83) were identified successfully.
文摘目的探讨基质辅助激光解吸附电离飞行时间质谱(matrix assisted laser desorption ionization ti me of flight mass spectrometry,MALDI-TOF-MS)对宫颈鳞癌(squamous carcinoma of thecervix,SCC)组织切片直接分析,获取宫颈鳞癌早期浸润的分子标志物的有效性。方法利用超微量点样枪将化学基质直接点样到宫颈鳞癌组织切片上,然后利用基质辅助激光解吸附电离飞行时间质谱直接扫描宫颈鳞癌组织切片,分析宫颈鳞癌组织蛋白质分子。结果获得的25种蛋白质质谱峰强度比较,差异有显著意义(P<0.05)。其中7种差异蛋白质有分类意义,其质/核(M/Z)分别为:m/z3433,m/z4786,m/z10092,m/z10835,m/z15114,m/z15854和m/z16345。差异蛋白质m/z3433,m/z15114,m/z15854在宫颈鳞癌鳞状上皮组织高度表达,可作为宫颈鳞癌早期诊断的标志蛋白;差异蛋白质m/z16345在宫颈鳞癌的间质组织高度表达,可作为宫颈鳞癌早期间质浸润和扩散诊断的标志蛋白。结论利用基质辅助激光解吸附电离飞行时间质谱技术成功获取一组差异蛋白质。该差异蛋白组可望作为宫颈鳞癌早期诊断的标志蛋白。
